The present study is done to perform the optimal design of structural components buildings against unwanted wind load exerted on their outer face. To this end, case research wall made a one-floor building modeled as rectangular plate with only one free edge and three clamped ones. It assumed that sandwich whose core auxetic material dace-sheets are reinforced nanoparticles graphene platelets (GPL). Differential equations governing system's motion obtained within background plate's...

Abstract Retinal degeneration (RD) is an irreversible blinding disease that seriously affects patients’ daily activities and mental health. Targeting hyperactivated microglia regulating polarization are promising strategies for treating the disease. Mesenchymal stem cell (MSC) transplantation proven to be effective treatment due its immunomodulatory regenerative properties. However, low efficiency of migration integration MSCs remains a major obstacle clinical use. The goal this study...

Steam curing is a widely used method in the production of industrial precast concrete but it often leads to thermal damage that negatively impacts material’s long-term durability and mechanical strength. The use supplementary cementitious materials (SCMs) has shown considerable promise improving pore structure alleviating these adverse effects. This study employs high-resolution X-ray computed tomography (X-CT) thoroughly assess how steam temperatures various subsequent regimes influence...

Lung cancer is one of the leading triggers for death worldwide. In this study, relationship aberrantly methylated and differentially expressed genes in lung adenocarcinoma (LUAD) with prognosis was investigated, 5 feature were identified eventually. Specifically, we firstly downloaded LUAD‐related mRNA expression profile (including 57 normal tissue samples 464 LUAD samples) Methy450 data 32 373 from TCGA database. The package “limma” used to screen genes, which intersected identifying...

Abstract Sustained androgen receptor (AR) signaling and apoptosis evasion are among the main hurdles of castration‐resistant prostate cancer (CRPC) treatment. We designed synthesized isothiocyanate (ITC)‐containing hybrid AR antagonist (ITC‐ARi) rationally combined ITC‐ARi with GSH synthesis inhibitor buthionine sulfoximine (BSO) to efficiently downregulate AR/AR splice variant induce ferroptosis in CRPC cells. The representative 13 is an ligand that contains N ‐acetyl cysteine‐masked ITC...

Increasing evidence shows that there is an interaction between mitogen-activated protein kinase and Wnt signaling their plays important roles in a variety of cellular processes. However, how the two interacts not clear. In this study, we found β-catenin expression was strikingly increased intestinal normal mucosa tumors c-Jun N-terminal (JNK) 1-deficient mice by immunohistochemical staining both transcriptional activity were significantly upregulated JNK1-deficient mouse embryonic...

Autologous melanocytes can be expanded in vitro, allowing the treatment of large lesions vitiligo one session. Theoretically, this procedure could provide a higher donor/recipient size ratio (DR ratio) compared with that noncultured cell transplantation (with DR < 1 : 10). However, exact obtained from has not been reported.To study whether cultured pure at high is as efficient low ratio.One hundred and two patients were treated by divided into groups: group, including ≤ 10 (mean 8, 35 cases)...

Aims . Acute-on-chronic liver failure (ACLF) and acute (ALF) are similar in many respects during their exacerbation; however, ACLF generally has a poorer prognosis. We aimed to investigate the role dynamic changes of regulatory T cell (Treg) helper 17 (Th17) proportions progress. Methods All rats were classified into two groups randomly: group ALF (control group). The rat model was preestablished by intraperitoneal injection carbon tetrachloride for 2 months. Then injury induced combined...

Inorganic arsenic (iAs) is an environmental toxicant associated with increased risk of prostate cancer in chronically exposed populations worldwide. However, the biological mechanisms underlying iAs-induced carcinogenesis remain unclear. We studied how iAs affects normal human stem-progenitor cells (PrSPCs) and drives transformation interrogated molecular involved. PrSPCs were enriched by spheroid culture from primary or immortalized epithelial cells, their differentiation capability was...

Abstract Background WNT signaling is implicated in embryonic development, and adult tissue homeostasis, while its deregulation evident disease. This study investigates the unique roles of canonical WNT10B both normal prostate development cancer (PCa) progression. Methods Organ culture rat ventral prostates (VPs) were used to Wnt10b ontogeny growth effect protein. PB‐SV40 LTag VPs utilized for Wnt expression polymerase chain reaction (PCR) array immunohistochemistry. Human localized PCa...

The aim of the present study was to investigate role microRNA (miR)‑21 in regulating collagen I and Smad7 expression activated rat hepatic stellate cells (HSCs). Rat HSCs were isolated by single‑step density gradient centrifugation with Nycodenz. Cellular content miR‑21, SMAD7, α‑smooth muscle actin (α‑SMA), type alpha 1 (COLLA1) COLL 2 (A2) mRNA examined reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), cellular α‑SMA protein detected western blotting. Binding miR‑21...

The progression of castration-resistant prostate cancer (CRPC) still relies on the function androgen receptor (AR), achieved by evolving mechanisms to reactivate AR signaling under hormonal therapy. Histone deacetylase inhibitors (HDACis) disrupt cytoplasmic chaperone heat shock protein 90 (Hsp90) via HDAC6 inhibition, leading degradation and growth suppression (PCa) cells. However, current HDACis are not effective in clinical trials treating CRPC.We designed hybrid molecules containing...

Abstract This research investigates the dynamic response and failure behavior of X80 steel pipelines under external contact explosion loads, a critical concern for oil gas pipeline safety due to increased terrorist activities. The characteristics are critically analyzed ten different conditions explosive charges, providing detailed evaluation damage mechanisms, including denting perforation. study finds that wall thickness significantly influences blast resistance pipelines, with thicker...

Hypoxia serves a critical role in the pathogenesis of liver fibrosis. Hypoxia‑inducible factor 1α (HIF1‑α) is induced when cells are exposed to low O2 concentrations. Recently, it has been suggested that Rho‑associated coiled‑coil‑forming kinase 1 (ROCK1) may be an important HIF1‑α regulator. In present study, was analyzed whether crosstalk between and ROCK1 regulates cell proliferation collagen synthesis hepatic stellate (HSCs) under hypoxic conditions. For this purpose, rat HSC line...

Activation of hepatic stellate cells (HSCs) is a pivotal event during fibrogenesis. Activated HSCs are the main source collagen and other extracellular matrix (ECM) components, emerging antifibrotic therapies aimed at preventing ECM synthesis deposition. MicroRNAs (miRNAs) have been demonstrated to exert regulatory effects on HSC activation synthesis. In present study, HSC‑T6 rat cell line was transiently transfected with miRNA (miR)‑203 mimic, which an artificial that enhances function...

Single prostate stem cells can generate and progenitor to form prostaspheres in 3D culture. Using a prostasphere-based label retention assay, we recently identified keratin 13 (KRT13)-enriched at single-cell resolution, distinguishing them from daughter progenitors. Herein, characterized the epithelial cell lineage hierarchy using RNA-seq analysis. Keratin profiling revealed three clusters of label-retaining cells; cluster I represents quiescent (PSCA, CD36, SPINK1, KRT13/23/80/78/4...