A5053271260- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Developmental Biology and Gene Regulation
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Genetics, Aging, and Longevity in Model Organisms
University of North Carolina at Chapel Hill
2021-2024
UNC Lineberger Comprehensive Cancer Center
2021
University of Wisconsin–Madison
2015-2020
Abstract Proteins bind and control mRNAs, directing their localization, translation stability. Members of the PUF family RNA-binding proteins multiple mRNAs in a single cell, play key roles development, stem cell maintenance memory formation. Here we identified mRNA targets S. cerevisiae protein, Puf5p, by ultraviolet-crosslinking-affinity purification high-throughput sequencing (HITS-CLIP). The binding sites recognized Puf5p are diverse, with variable spacer lengths between two specific...
Abstract It has been suggested that transcription factor binding is temporally dynamic, and changes in determine transcriptional output. Nonetheless, this model based on relatively few examples which assayed at multiple developmental stages. The essential Grainy head (Grh) conserved from fungi to humans, controls epithelial development barrier formation numerous tissues. Drosophila melanogaster, possess a single grainy (grh) gene, provide an excellent system study factor. To whether distinct...
In nearly all metazoans, the earliest stages of development are controlled by maternally deposited mRNAs and proteins. The zygotic genome becomes transcriptionally active hours after fertilization. Transcriptional activation during this maternal-to-zygotic transition (MZT) is tightly coordinated with degradation provided mRNAs. Drosophila melanogaster, transcription factor Zelda plays an essential role in widespread genome. While expression required both zygotically, mechanisms which it...
The dramatic changes in gene expression required for development necessitate the establishment of cis-regulatory modules defined by regions accessible chromatin. Pioneer transcription factors have unique property binding closed chromatin and facilitating these regions. Nonetheless, much how pioneer coordinate accessibility during remains unknown. To determine whether pioneer-factor function is intrinsic to protein or pioneering activity developmentally modulated, we studied highly conserved,...
Abstract Mutating replication-dependent (RD) histone genes is an important tool for understanding chromatin-based epigenetic regulation. Deploying this in metazoan models particularly challenging because RD histones these organisms are typically encoded by many genes, often located at multiple loci. Such gene arrangements make the ability to generate homogenous mutant genotypes site-specific editing quite difficult. Drosophila melanogaster provides a solution problem organized into single...
Asynchronous replication of chromosome domains during S phase is essential for eukaryotic genome function, but the mechanisms establishing which replicate early versus late in different cell types remain incompletely understood. Intercalary heterochromatin very both diploid chromosomes dividing cells and endoreplicating polytene where they are also underreplicated. Drosophila SNF2-related factor SUUR imparts locus-specific underreplication chromosomes. negatively regulates DNA fork...
ABSTRACT In nearly all metazoans, the earliest stages of development are controlled by maternally deposited mRNAs and proteins. The zygotic genome becomes transcriptionally active hours after fertilization. Transcriptional activation during this maternal-to-zygotic transition (MZT) is tightly coordinated with degradation provided mRNAs. Drosophila melanogaster, transcription factor Zelda plays an essential role in widespread genome. While expression required both zygotically, mechanisms...
Mutating replication-dependent (RD) histone genes is an important tool for understanding chromatin-based epigenetic regulation. Deploying this in metazoans particularly challenging because RD histones these organisms are typically encoded by many genes, often located at multiple loci. Such gene arrangements make the ability to generate homogenous mutant genotypes site-specific editing quite difficult. Drosophila melanogaster provides a solution problem organized into single large tandem...
Abstract The dramatic changes in gene expression required for development necessitate the establishment of cis- regulatory modules defined by regions accessible chromatin. Pioneer transcription factors have unique property binding closed chromatin and facilitating these regions. Nonetheless, much how pioneer coordinate accessibility during remains unknown. To determine whether pioneer-factor function is intrinsic to protein or pioneering activity developmentally modulated, we studied highly...
Abstract Asynchronous replication of chromosome domains during S phase is essential for eukaryotic genome function, but the mechanisms establishing which replicate early versus late in different cell types remain incompletely understood. Drosophila SNF2-related factor SUUR imparts under- late-replicating intercalary heterochromatin polytene chromosomes. negatively regulates DNA fork progression; however, its mechanism action remains obscure. Here we developed a novel method termed MS-Enabled...