A5053548185- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Developmental Biology and Gene Regulation
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Genetics, Aging, and Longevity in Model Organisms
- Insect Resistance and Genetics
- Reproductive Biology and Fertility
- Hippo pathway signaling and YAP/TAZ
- Renal and related cancers
- Microtubule and mitosis dynamics
University of Wisconsin–Madison
2017-2024
Following fertilization, the genomes of germ cells are reprogrammed to form totipotent embryo. Pioneer transcription factors essential for remodeling chromatin and driving initial wave zygotic gene expression. In Drosophila melanogaster , pioneer factor Zelda is development through this dramatic period reprogramming, known as maternal-to-zygotic transition (MZT). However, it was unknown whether additional were required transition. We identified an maternally encoded MZT, GAGA Factor (GAF)....
In nearly all metazoans, the earliest stages of development are controlled by maternally deposited mRNAs and proteins. The zygotic genome becomes transcriptionally active hours after fertilization. Transcriptional activation during this maternal-to-zygotic transition (MZT) is tightly coordinated with degradation provided mRNAs. Drosophila melanogaster, transcription factor Zelda plays an essential role in widespread genome. While expression required both zygotically, mechanisms which it...
During Drosophila embryogenesis, the essential pioneer factor Zelda defines hundreds of cis-regulatory regions and in doing so reprograms zygotic transcriptome. While is later development, it unclear how ability to define shaped by cell-type-specific chromatin architecture. Asymmetric division neural stem cells (neuroblasts) fly brain provide an excellent paradigm for investigating functions this factor. We show that synergistically with Notch maintain neuroblasts undifferentiated state....
Abstract Following fertilization, the unified germ cells rapidly transition to a totipotent embryo. Maternally deposited mRNAs encode proteins necessary for this reprogramming as zygotic genome remains transcriptionally quiescent during initial stages of development. The transcription factors required activate are among these maternally and robustly translated following fertilization. In Drosophila, mRNA encoding Zelda, major activator genome, is not until 1 h after Here we demonstrate that...
Coordinated regulation of gene activity by transcriptional and translational mechanisms poise stem cells for a timely cell-state transition during differentiation. Although important all stemness-to-differentiation transitions, mechanistic understanding the fine-tuning transcription is lacking due to compensatory effect control. We used intermediate neural progenitor (INP) identity commitment define that fine-tune stemness in fly (neuroblasts). demonstrate factor FruitlessC (FruC) binds...
ABSTRACT In nearly all metazoans, the earliest stages of development are controlled by maternally deposited mRNAs and proteins. The zygotic genome becomes transcriptionally active hours after fertilization. Transcriptional activation during this maternal-to-zygotic transition (MZT) is tightly coordinated with degradation provided mRNAs. Drosophila melanogaster, transcription factor Zelda plays an essential role in widespread genome. While expression required both zygotically, mechanisms...
Abstract Following fertilization, the genomes of germ cells are reprogrammed to form totipotent embryo. Pioneer transcription factors essential for remodeling chromatin and driving initial wave zygotic gene expression. In Drosophila melanogaster , pioneer factor Zelda is development through this dramatic period reprogramming, known as maternal- to-zygotic transition (MZT). However, it was unknown whether additional were required transition. We identified an maternally encoded MZT, GAGA...
Abstract During Drosophila embryogenesis, the essential pioneer factor Zelda defines hundreds of cis -regulatory regions and in doing so reprograms zygotic transcriptome. While is later development, it unclear how ability to define shaped by cell-type-specific chromatin architecture. Asymmetric division neural stem cells (neuroblasts) fly brain provide an excellent paradigm for investigating functions this factor. We show that synergistically with Notch maintain neuroblasts undifferentiated...
Abstract Following fertilization, the unified germ cells rapidly transition to a totipotent embryo. Maternally deposited mRNAs encode proteins necessary for this reprogramming as zygotic genome remains transcriptionally quiescent during initial stages of development. The transcription factors required activate are among these maternally and robustly translated following fertilization. In Drosophila , mRNA encoding Zelda, major activator genome, is not until one hour after Here we demonstrate...
Abstract Coordinated regulation of stemness gene activity by transcriptional and translational mechanisms poise stem cells for a timely cell-state transition during differentiation. Although important all stemness-to-differentiation transitions, mechanistic understanding the fine-tuning transcription is lacking due to compensatory effect control. We used intermediate neural progenitor (INP) identity commitment define that fine-tune in fly (neuroblasts). demonstrate factor Fruitless C (Fru )...