A5025786011- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- DNA Repair Mechanisms
- Developmental Biology and Gene Regulation
University of Wisconsin–Madison
2020-2023
Following fertilization, the genomes of germ cells are reprogrammed to form totipotent embryo. Pioneer transcription factors essential for remodeling chromatin and driving initial wave zygotic gene expression. In Drosophila melanogaster , pioneer factor Zelda is development through this dramatic period reprogramming, known as maternal-to-zygotic transition (MZT). However, it was unknown whether additional were required transition. We identified an maternally encoded MZT, GAGA Factor (GAF)....
Abstract To maintain cellular identities during development, gene expression profiles must be faithfully propagated through cell generations. The reestablishment of patterns upon mitotic exit is mediated, in part, by transcription factors (TF) bookmarking. However, the mechanisms and functions TF bookmarking early embryogenesis remain poorly understood. In this study, taking advantage naturally synchronized mitoses Drosophila embryos, we provide evidence that GAGA pioneer factor (GAF) acts...
Abstract Meier-Gorlin syndrome (MGS) is a recessive disorder caused by mutations in genes associated with DNA replication. Despite the fact these proteins are essential for replication every cell, patients MGS have tissue-specific defects... rare characterized number of distinct developmental defects. Genes encoding members origin recognition complex (ORC) and additional (CDC6, CDT1, GMNN, CDC45, MCM5, DONSON) mutated individuals diagnosed MGS. The role ORC to license origins during G1 phase...
Abstract Following fertilization, the genomes of germ cells are reprogrammed to form totipotent embryo. Pioneer transcription factors essential for remodeling chromatin and driving initial wave zygotic gene expression. In Drosophila melanogaster , pioneer factor Zelda is development through this dramatic period reprogramming, known as maternal- to-zygotic transition (MZT). However, it was unknown whether additional were required transition. We identified an maternally encoded MZT, GAGA...
Abstract/Summary The eukaryotic genome is organized to enable the precise regulation of gene expression required for development. This organization established during early development when embryo transitions from a fertilized germ cell totipotent zygote. To understand factors and processes that drive genomic organization, we focused on pioneer factor GAGA (GAF) embryonic in Drosophila. GAF transcriptionally activates zygotic localized subnuclear foci. We show this non-uniform distribution...
Abstract To maintain cellular identities during development, gene expression profiles must be faithfully propagated through cell generations. The reestablishment of patterns upon mitotic exit is thought to mediated, in part, by bookmarking transcription factors (TF). However, the mechanisms and functions TF early embryogenesis remain poorly understood. In this study, taking advantage naturally synchronized mitoses Drosophila embryos, we provide evidence that pioneer-like factor GAF acts as...