A5081420809- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- DNA and Nucleic Acid Chemistry
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- DNA Repair Mechanisms
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Genomics and Rare Diseases
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Plant Molecular Biology Research
- vaccines and immunoinformatics approaches
- Gene expression and cancer classification
- Machine Learning in Bioinformatics
- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
Institut de Génétique Moléculaire de Montpellier
2015-2024
Université de Montpellier
2017-2024
Centre National de la Recherche Scientifique
2015-2024
Centre d’Immunologie de Marseille-Luminy
2009-2016
Inserm
2008-2016
Aix-Marseille Université
2008-2015
Délégation Provence et Corse
2011
Institut de Neurobiologie de la Méditerranée
2011
Utrecht University
2002-2006
University Medical Center Utrecht
2002-2005
Genetic programming for self-renewal Instead of repopulating themselves from tissue-resident stem cell pools like most types differentiated cells, tissue macrophages maintain by self-renewing. The underlying genetic programs that allow this, however, are unknown. Soucie et al. now report in at homeostasis, a pair transcription factors (MafB and c-Maf) bind to repress the enhancers genes regulating self-renewal. When need replenish their stocks, example response injury, they transiently...
One clear hallmark of mammalian promoters is the presence CpG islands (CGIs) at more than two-thirds genes, whereas TATA boxes are only present a minority promoters. Using genome-wide approaches, we show that GC content and CGIs major promoter elements in cells, able to govern open chromatin conformation support paused transcription. First, define three classes with distinct transcriptional directionality pausing properties correlate their content. We further analyze direct influence on...
To unveil the still-elusive nature of metazoan replication origins, we identified them genome-wide and at unprecedented high-resolution in mouse ES cells. This allowed initiation sites (IS) zones (IZ) to be differentiated. We then characterized their genetic signatures organization integrated these data with 43 chromatin marks factors. Our results reveal that origins can grouped into three main classes distinct organization, environment, sequence motifs. Class 1 contains relatively isolated,...
Cell-type specific regulation of gene expression requires the activation promoters by distal genomic elements defined as enhancers. The identification and characterization enhancers are challenging in mammals due to their genome complexity. Here we develop CapStarr-Seq, a novel high-throughput strategy quantitatively assess enhancer activity mammals. This approach couples capture regions interest previously developed Starr-seq technique. Extensive assessment CapStarr-seq demonstrates...
Divergent transcription is a wide-spread phenomenon in mammals. For instance, short bidirectional transcripts are hallmark of active promoters, while longer can be detected antisense from genes conditions where the RNA degradation machinery inhibited. Moreover, many described long non-coding RNAs (lncRNAs) transcribed coding gene promoters. However, general significance divergent lncRNA/mRNA pair still poorly understood. Here, we used strand-specific RNA-seq with high sequencing depth to...
Abstract Promoter-proximal pausing of RNA polymerase II is a key process regulating gene expression. In latent HIV-1 cells, it prevents viral transcription and essential for latency maintenance, while in acutely infected cells the factor Tat releases paused to induce Pausing fundamental HIV-1, but how contributes bursting stochastic reactivation unclear. Here, we performed single molecule imaging transcription. We developed quantitative analysis method that manages multiple time scales from...
Abstract To maintain cellular identities during development, gene expression profiles must be faithfully propagated through cell generations. The reestablishment of patterns upon mitotic exit is mediated, in part, by transcription factors (TF) bookmarking. However, the mechanisms and functions TF bookmarking early embryogenesis remain poorly understood. In this study, taking advantage naturally synchronized mitoses Drosophila embryos, we provide evidence that GAGA pioneer factor (GAF) acts...
The structure of the yeast RNA polymerase (pol) III was investigated by exhaustive two-hybrid screening using a library random genomic fragments fused to Gal4 activation domain. This procedure allowed us identify contacts between individual polypeptides, localize contact domains, and deduce protein-protein interaction map multisubunit enzyme. In all but one case, pol subunits were able interact in vivo with or sometimes two partner enzyme TFIIIC. Four that are common I, II, (ABC27, ABC14.5,...
Accurate gene expression requires the precise control of mRNA levels, which are determined by relative rates nuclear (pre-)mRNA synthesis and processing, cytoplasmic turnover. A key step in degradation is removal poly(A) tail, involves several deadenylases including components Ccr4-Not complex. Here, we focused on role human paralogues CNOT7 (hCaf1/Caf1a) CNOT8 (hPop2/Caf1b/Calif), possess deadenylase activity mediated DEDD nuclease domains. We show that efficient proliferation both...
In mammals, the carboxy-terminal domain (CTD) of RNA polymerase (Pol) II consists 52 conserved heptapeptide repeats containing consensus sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Post-translational modifications CTD coordinate transcription cycle and various steps mRNA maturation. Here we describe Tyr1 phosphorylation (Tyr1P) as a hallmark promoter (5′ associated) Pol in mammalian cells, contrast to what was described yeast. Tyr1P is predominantly found antisense orientation at promoters...
Termination of transcription is important for establishing gene punctuation marks. It also critical suppressing many the pervasive events occurring throughout eukaryotic genomes and coupling their RNA products to efficient decay. In human cells, ARS2 protein has been implicated in such function as its depletion causes transcriptional read-through selected terminators because it physically interacts with ribonucleolytic nuclear exosome. Here, we study role on metabolism genome wide. We show...
T-cell acute lymphoblastic leukaemias (T-ALL) are aggressive malignant proliferations characterized by high relapse rates and great genetic heterogeneity. TAL1 is amongst the most frequently deregulated oncogenes. Yet, over half of TAL1(+) cases lack lesions, suggesting unrecognized (epi)genetic deregulation mechanisms. Here we show that normally silenced in lineage, polycomb H3K27me3-repressive mark focally diminished T-ALLs. Sequencing reveals >20% monoallelic patients without previously...