A5059271453- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Natural product bioactivities and synthesis
- Alkaloids: synthesis and pharmacology
- Fungal and yeast genetics research
- Ginseng Biological Effects and Applications
- CRISPR and Genetic Engineering
- Phytochemistry and Biological Activities
- Mitochondrial Function and Pathology
- Microbial Natural Products and Biosynthesis
- Plant-based Medicinal Research
- Analytical Chemistry and Chromatography
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
University of Wisconsin–Madison
2019-2024
University of Illinois Urbana-Champaign
2018-2021
The pioneer event in eukaryotic DNA replication is binding of chromosomal by the origin recognitioncomplex (ORC). ORC-DNA complex directs formation origins, specific regions where synthesis initiates. In all eukaryotes, incompletely understood features chromatin promote binding. Here, we uncover a role for Fkh1 (Forkhead homolog) protein and its forkhead associated (FHA) domain promoting ORC-origin activity at subset origins Saccharomyces cerevisiae. Several FHA-dependent examined required...
Loganin is an iridoid glycoside of interest as both intermediate in the biosynthesis indole alkaloids plants and a bioactive compound itself. Loganic acid methyltransferase catalyzes methylation monoterpenoid precursor to produce loganin demonstrates stereospecificity for (6S,7R) substrate. We have biochemically characterized this biocatalyst elucidated basis its strict substrate specificity. These studies could help facilitate design new classes pharmaceutical interest.
Abstract Meier-Gorlin syndrome (MGS) is a recessive disorder caused by mutations in genes associated with DNA replication. Despite the fact these proteins are essential for replication every cell, patients MGS have tissue-specific defects... rare characterized number of distinct developmental defects. Genes encoding members origin recognition complex (ORC) and additional (CDC6, CDT1, GMNN, CDC45, MCM5, DONSON) mutated individuals diagnosed MGS. The role ORC to license origins during G1 phase...
In Saccharomyces cerevisiae , the forkhead (Fkh) transcription factor Fkh1 (forkhead homolog) enhances activity of many DNA replication origins that act in early S-phase (early origins). Current models posit acts directly to promote these origins’ by binding origin-adjacent sites (FKH sites). However, post-DNA functions uses origin are poorly understood. contains a conserved FHA associated) domain, protein-binding module with specificity for phosphothreonine (pT)-containing partner proteins....
Abstract In Saccharomyces cerevisiae , the forkhead (Fkh) transcription factor Fkh1 (forkhead homolog) enhances activity of many DNA replication origins that act in early S-phase (early origins). Current models posit acts directly to promote these origins’ by binding origin-adjacent sites (FKH sites). However, post-DNA functions uses origin are poorly understood. contains a conserved FHA associated) domain, protein-binding module with specificity for phosphothreonine (pT)-containing partner...
Abstract The pioneer event in eukaryotic DNA replication is binding of chromosomal by the origin recognition complex (ORC), which directs formation origins, specific regions where will be unwound for initiation synthesis. In all eukaryotes, incompletely understood features chromatin promote ORC-DNA binding. Here, we uncover a role Fkh1 (forkhead homolog) protein, and, particular, its forkhead associated (FHA) domain promoting ORC-origin and activity at subset origins Saccharomyces...
Abstract Meier-Gorlin syndrome is a rare recessive disorder characterized by number of distinct developmental defects, including primordial dwarfism, small ears, and or missing patella. Genes encoding members the origin recognition complex (ORC) additional proteins essential for DNA replication (CDC6, CDT1, GMNN, CDC45, MCM5) are mutated in individuals diagnosed with MGS. The primary role ORC to license origins during G1 phase cell cycle, but it also plays roles cilia development,...