A5074816151- Legume Nitrogen Fixing Symbiosis
- Soil Management and Crop Yield
- Plant-Microbe Interactions and Immunity
- Nematode management and characterization studies
- Hemoglobin structure and function
- Plant Disease Management Techniques
- Heavy metals in environment
- Metabolism and Genetic Disorders
- Metal-Catalyzed Oxygenation Mechanisms
- Mine drainage and remediation techniques
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- Insect Utilization and Effects
- Phosphorus and nutrient management
- Growth and nutrition in plants
- Plant Parasitism and Resistance
- NMR spectroscopy and applications
- Soil and Land Suitability Analysis
- Pesticide and Herbicide Environmental Studies
- Protein Structure and Dynamics
- Heme Oxygenase-1 and Carbon Monoxide
- Fungal and yeast genetics research
- Recycling and utilization of industrial and municipal waste in materials production
- Plant Pathogens and Fungal Diseases
- Groundwater flow and contamination studies
Instituto Nacional de Investigação Agrária e Veterinária
2003-2024
Ministério da Agricultura
2007
Universidade Nova de Lisboa
1989-2003
University of Georgia
1992-1995
Organismo Autónomo Parques Nacionales
1989
Hermetia illucens L., known as Black Soldier Fly (BSF) appear an opportunity to reuse vegetable by-products, it is easy reproduce and can be reared in agricultural side streams, allowing the production of both, animal feed (the larvae, after recycling vegetal debris) soil organic fertilizer (insect frass). Although several fertilizers, from long ago, have been used agriculture, there yet a paucity experimental data on evaluation fertilization potential BSF larvae frass (BSFF). The present...
An NADH--rubredoxin oxidoreductase previously isolated from Desulfovibrio gigas [LeGall, J. (1968) Ann. Inst. Pasteur 114, 109-115] has now been fully purified and further characterized. It contains two subunits of 27 kDa 32 kDa. With mid-point redox potentials -295 mV -325 mV, this FMN- FAD-containing protein can induce the specific reduction D. rubredoxin. In contrast, rubredoxins other species or desulforedoxin show very low reaction rates with same enzyme. The phylogenetic significance...
To search for culturable Burkholderia species associated with annual ryegrass in soils from natural pastures Portugal, plant growth‐promoting effects. Annual seedlings were used to trap two different laboratory conditions. A combined approach using genomic fingerprinting and sequencing of 16S rRNA recA genes resulted the identification strains belonging graminis, fungorum cepacia complex. Most able solubilize mineral phosphate synthesize indole acetic acid; some them could produce...
The biochemical response to oxygen of the strictly anaerobic sulfate-reducing bacterium Desulfovibrio gigas was studied with goal elucidating survival strategies in oxic environments. Cultures D. on medium containing lactate and sulfate were exposed (concentration 5-120 micro M). Growth fully inhibited by oxygen, but cultures resumed growth as soon they shifted back anoxic conditions. Following 24 h exposure rate high 70 % rates observed before oxygenation. Catalase levels activity enhanced...
Numerous plastic products are used in agriculture, including containers, packaging, tunnels, drip irrigation tubing, and mulches. Large amounts of plastics as mulches on the soil surface for vegetable fruit production (tomato, cucumber, watermelon, strawberry, vine) to reduce weed competition, increase water fertilizer use efficiency, enhance crop yield. Portugal uses around 4500 t/year polyethylene cover approximately 23,000 ha agricultural land, only a small amount is recovered recycling...
The sulfate-reducing bacterium Desulfovibrio gigas accumulates large amounts of polyglucose as an endogenous carbon and energy reserve. In the absence exogenous substrates, intracellular polysaccharide was utilized, conserved in process (H. Santos, P. Fareleira, A. V. Xavier, L. Chen, M.-Y. Liu, J. LeGall, Biochem. Biophys. Res. Commun. 195:551-557, 1993). When external electron acceptor not provided, degradation by cell suspensions D. yielded acetate, glycerol, hydrogen, ethanol. A detailed...
The aim of this study was to evaluate the potential agronomic value black soldier fly larvae frass (BSFF) as an organic fertiliser in short-cycle crops, using lettuce test plant. Treatments consisted applying different fertilisers (BSFF and mineral) at doses combinations. experiment carried out for 42 days plants were analysed terms biomass production, while soil chemically characterised before after fertilisation, order assess residual nutrient concentrations. In addition, microbial...
The structure of a novel diphosphodiester compound recently detected in Desulfovibrio desulfuricans cells [Santos, Fareleira, Pedregal, LeGall & Xavier (1991) Eur. J. Biochem. 201, 283-287] was fully elucidated using combination n.m.r. techniques aqueous and methanolic solutions. metabolite identified as 3-methyl-1,2,3,4-tetrahydroxybutane-1,3-cyclic bisphosphate, the minimum energy conformation is presented. two chiral centres have relative configuration RS.
Two isolates, designated B15.09-116T and B15.09-124, were recovered from bottled mineral water in Portugal. Based on 16S rRNA gene sequence analysis, these strains related most closely to species of the genus Derxia (belonging family Alcaligenaceae) with pairwise similarities 93.0-93.6 %. The isolates not pigmented formed Gram-stain-negative, short, motile rod-shaped cells. organisms strictly aerobic, oxidase-positive catalase-negative. These also fixed N2. major polar lipids...
(1) Aims: Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of native African trees Colophospermum mopane Combretum apiculatum three landscapes Limpopo National Park (Mozambique), subjected to two fire regimes. (2) Methods: Bacterial communities were identified through Illumina Miseq sequencing 16S rRNA gene amplicons, followed by culture dependent methods isolate plant growth-promoting bacteria (PGPB). Plant traits cultivable fraction further analyzed. To...
(1) Background: the Miombo woodlands comprise most important vegetation from southern Africa and are dominated by tree legumes with an ecology highly driven fires. Here, we report on characterization of bacterial communities rhizosphere Brachystegia boehmii in different soil types areas subjected to regimes. (2) Methods: were identified through Illumina MiSeq sequencing (16S rRNA). Vigna unguiculata was used as a trap capture nitrogen-fixing bacteria culture-dependent methods selective media...
Carbon and phosphorus metabolism of cell suspensions Methanosarcina barkeri strain MS (DSM 800), grown on methanol, were probed in vivo by NMR. The experimental conditions, which involved thick suspensions, did not significantly affect the efficiency rate methanol uptake cells. Following exposure to an acidification both intracellular extracellular spaces was observed a gradient 0.5 pH units across cytoplasmic membrane determined from 31 P‐NMR data. High levels ATP up 4 mM detected. ADP...
The phosphorus metabolism of sulfate‐reducing bacteria was, for the first time, probed by in vivo 31 P NMR. A novel phosphoric anhydride diester compound was detected Desulfovibrio desulfuricans ATCC 27774 at intracellular concentration up to 5 mM. has been extracted and partially purified anion‐exchange chromatography analysed P, 13 C 1 H These studies show that phosphorus‐containing is formed five carbon atoms probably cyclic, with a M r approximately 300. Various strains were examined...
In recent decades there has been growing concern around heavy metals and metalloid contamination in soil. Arsenic (As) is a ubiquitous trace metalloid. The high levels of this soils are consequence human activities also from natural inputs. general, the biodiversity microorganisms plants decreases drastically contaminated soils. knowledge that some leguminous plants, mainly certain species <italic>Lotus</italic>, well such attracted our attention for studying symbioses adapted to harsh...
In the present case study, aim is to show an example of effect on residual fertility parameters (particularly with regard re-sprouting plants after harvesting aerial part), taken from a pot experiment, where modalities without fertilization, mineral organic fertilization and mixed were contrasted. Within this context it possible conclude following: Mineral alone, leads drastic inhibition plant re-sprouting, that even in situations (mineral + organic) considerable; terms microbiological...