A5049692322- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- Lipid Membrane Structure and Behavior
- Diet and metabolism studies
- Pancreatic and Hepatic Oncology Research
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Metabolism, Diabetes, and Cancer
- FOXO transcription factor regulation
University of Toronto
2022-2023
Hospital for Sick Children
2022
University of British Columbia
2019-2021
Lipid nanoparticles (LNPs) are the most clinically advanced delivery vehicles for RNA and have enabled development of RNA-based drugs such as mRNA COVID-19 vaccines. Functional by an LNP greatly depends on inclusion ionizable lipid, small changes to these lipid structures can significantly improve delivery. However, structure–function relationships between lipids poorly understood, especially LNPs administered intramuscularly. Here, we show that iterative design a novel series generates key...
Lipid nanoparticles (LNPs) and ribonucleic acid (RNA) technology are highly versatile tools that can be deployed for diagnostic, prophylactic, therapeutic applications. In this report, supramolecular chemistry concepts incorporated into the rational design of a new ionizable lipid, C3-K2-E14, systemic administration. This lipid incorporates cone-shaped structure intended to facilitate cell bilayer disruption, three tertiary amines improve RNA binding. Additionally, hydroxyl amide motifs...
Endothelia determine blood-to-tissue solute delivery, yet glucose transit is poorly understood. To illuminate mechanisms, we tracked [3H]-2-deoxyglucose (2-DG) in human adipose-tissue microvascular endothelial cells. 2-DG uptake was largely facilitated by the transporters GLUT1 and GLUT3. Once cytosol, >80% of became phosphorylated ∼20% incorporated into glycogen, suggesting that transported readily accessible to cytosolic enzymes. Interestingly, a fraction intracellular released over time...
Abstract Pancreas is a vital organ composed of exocrine and endocrine cells that aid digestion food regulate blood glucose levels. Perturbations in the function pancreatic leads to development life-burdening and/or threatening diseases such as diabetes cancer. Thus, it critical understand molecular check-points maintain normal pancreas physiology. MicroRNAs (miRNAs) are small non-coding RNAs involved regulating gene expression diseased tissues. Several miRNAs have tissue-specific patterns...