A5048219414- Telomeres, Telomerase, and Senescence
- DNA Repair Mechanisms
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- PARP inhibition in cancer therapy
- Ovarian cancer diagnosis and treatment
- Immunodeficiency and Autoimmune Disorders
- Clostridium difficile and Clostridium perfringens research
- Gastrointestinal Tumor Research and Treatment
- Mass Spectrometry Techniques and Applications
- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- HIV Research and Treatment
- Hemoglobin structure and function
- Immunotherapy and Immune Responses
- Tuberculosis Research and Epidemiology
- Long-Term Effects of COVID-19
- interferon and immune responses
- Immune responses and vaccinations
- Diabetes and associated disorders
- Cancer, Hypoxia, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Macrophage Migration Inhibitory Factor
- PI3K/AKT/mTOR signaling in cancer
Cedars-Sinai Medical Center
2025
University of Oxford
2020-2024
Jenner Institute
2020-2024
Southampton General Hospital
2023
Middlemore Hospital
2021-2022
The University of Melbourne
2020
Austin Health
2020
Peninsula Health
2020
Eastern Health
2020
Monash Health
2020
SummaryBackgroundA safe and efficacious vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), if deployed with high coverage, could contribute to the control of COVID-19 pandemic. We evaluated safety efficacy ChAdOx1 nCoV-19 in a pooled interim analysis four trials.MethodsThis includes data from ongoing blinded, randomised, controlled trials done across UK, Brazil, South Africa. Participants aged 18 years older were randomly assigned (1:1) or (meningococcal group A,...
During investigation of a gastroenteritis outbreak in chronic care institution, Norwalk virus was found stool specimens from two individuals and bacterial isolates presumptively identified as Bacillus cereus were isolated four (including one with virus) spice. Phage typing confirmed all clinical phage type 2. All subsequently B. thuringiensis when tested result related study (L. Leroux, personal communication). Eight 10 spice 4. showed cytotoxic effects characteristic enterotoxin-producing...
A safety and immunogenicity trial was conducted in vaccinia-immune vaccinia-naive human immunodeficiency virus (HIV)-uninfected adults who were randomized to receive 10(6) or 10(7) TCID50 of canarypox (ALVAC) vector expressing HIV-1MN gp160 10(5.5) ALVAC-rabies glycoprotein control at 0 1 2 months ALVAC-gp160 50 microg HIV-1SF2 recombinant (r) gp120 microfluidized emulsion 9 12 months; others received rgp120 0, 1, 6, months. All vaccines well-tolerated. Neither status before vaccination nor...
Abstract An NAD-specific glutamate dehydrogenase has been isolated from Blastocladiella emersonii, purified over 100-fold, and some of its kinetic parameters studied. Saturation curves with NAD+ as variable substrate were largely sigmoidal at high concentrations. plots for hyperbolic except low levels when activated the reaction. Double reciprocal velocity nonlinear. The intense activation by described a biphasic character in diagnostic plots. On other hand, substrates, NADH α-ketoglutarate,...
A SARS-CoV-2 controlled human infection model (CHIM) has been successfully established in seronegative individuals using a dose of 1×10
Abstract The substrates, NAD+, NADH, and to a lesser extent, glutamate, of freely reversible glutamic dehydrogenase from Blastocladiella emersonii, diverse allosteric modulators (AMP, ADP, Ca++, Mn++) have been shown bind the enzyme prevent it dissociating into monomers at alkaline pH, high ionic strength, under hydrodynamic shear. At fixed pH these ligands display varying magnitude effectiveness as follows: NADH) g Mn++ glutamate. dissociates irreversibly an active tetramer (mol wt 200,000...
A nicotinamide adenine dinucleotide-specific isocitric dehydrogenase has been isolated and purified 450-fold from Blastocladiella emersonii. The enzyme a monomeric molecular weight of 110,000 ± 10,000. It is capable aggregating to polymers ranging 200,000 500,000 higher at acid pH levels. At alkaline values, it exists as monomer. aggregated inactive the monomer active. Under influence various ligands (citrate, isocitrate, Mn++, Mg++), can be converted difference 1 unit (6.5 7.5) changes...
Bacillus cereus is responsible for an increasing number of food poisoning cases. By using 12 bacteriophages isolated from sewage, a typing scheme B. isolates outbreaks or sporadic cases was developed. The phages belonged to three morphotypes. Ten with contractile tails and icosahedral heads were members the Myoviridae family, two noncontractile Siphoviridae family. Phage 11 represented new species. It had isometric head very long tail wavy fibers one largest viruses known. vast majority 166...
A series of Clostridium perfringens-related gastrointestinal outbreaks occurred over a period several months among elderly, chronic care patients in psychiatric hospital. Several serotypes C. perfringens and many nontypeable isolates were found. The distribution certain the incidence detection enterotoxin fecal extracts related to wards on which resident (six involved). reported have or recurring incontinence diarrhea both. With background elevated spore counts diarrhea, only could provide...
D.E. MAHONY, R. AHMED AND S.G. JACKSON, 1992. Twenty‐one stool specimens obtained from persons implicated in two food poisoning outbreaks at the same institution Smith Falls, Ontario, were examined for Clostridium perfringens. Ninety‐two colonies of Cl. perfringens (3–5 per specimen) typed with antisera, bacteriocins and by plasmid analysis. They also tested vitro production bacteriocin enterotoxin. Sixteen 21 directly This was detected 13, five which individuals listed as ‘asymptomatic’...
Culture supernatants of 30 enterotoxin-producing Bacillus cereus isolates produced a characteristic progressive destruction McCoy cell monolayers. Enterotoxin-negative B. and other group 1 spp. caused no monolayer disruption. The tissue culture system appears to provide rapid screening assay for detection cereus.
The 1% or Less Campaign is an effective research-tested program for reducing saturated fat intake by encouraging individuals to switch low-fat milk. All published studies have been conducted in small communities with mostly White populations. 6-week intervention included a media campaign, public relations, and taste tests. effectiveness was measured using sales data cross-sectional telephone surveys. Survey results showed significant increase milk consumption from 30.2% 40.8% of drinkers ( p...
A 30-min fluorogenic test was developed for differentiation of members the Candida parapsilosis group from other species commonly encountered in clinical material. The substrate, 4-methylumbelliferyl-beta-D-glucoside, utilized to assay beta-glucosidase activity. total 50 C. isolates and 135 four were tested. Assay sensitivity specificity 100 99.3%, respectively. procedure adapted use with a spectrofluorometer.
The diagnostic application of an enzyme immunoassay for Clostridium perfringens type A enterotoxin was evaluated. Test results from 100 individuals associated with C. gastroenteritis outbreaks and 111 control were included. assay sensitivity 93.7%, the specificity 98.7%.
Aims The influence of human factors on safety in healthcare settings is well established, with targeted interventions reducing risk and enhancing team performance. In experimental early phase clinical research participant paramount safeguarded by guidelines, protocolized care staff training; however, the real‐world interaction implementation these risk‐mitigating measures has never been subjected to formal system‐based assessment. Methods Independent structured observations, systematic...