A5070204492- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Microbial Metabolism and Applications
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Enzyme-mediated dye degradation
- Biofuel production and bioconversion
- Microbial Community Ecology and Physiology
- Meat and Animal Product Quality
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Food Industry and Aquatic Biology
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Hydrolysis and Bioactive Peptides
- Escherichia coli research studies
- Microbial Metabolic Engineering and Bioproduction
- Food Chemistry and Fat Analysis
- Vibrio bacteria research studies
- Metal-Catalyzed Oxygenation Mechanisms
- Microbial bioremediation and biosurfactants
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
2009-2024
All-Russian Scientific Research Institute of Refrigeration Industry
2021-2024
Vladimir State University
2024
Pushchino Scientific Center for Biological Research
2023
Russian Academy of Sciences
1998-2023
The Russian Presidential Academy of National Economy and Public Administration
2021
ITMO University
2018
University of Portsmouth
2009
Rutgers, The State University of New Jersey
2009
Institute of Bioorganic Chemistry
2007
Restriction-modification (R-M) system Ecl18kI is representative of R-M systems whose coordinated transcription achieved through a separate DNA-binding domain the methyltransferase. M.Ecl18kI recognizes an operator sequence located in noncoding region that separates divergently transcribed R and M genes. Here we show that, contrary to previous predictions, two ecl18kI promoters are not divergent, but actually face one another. The binding its prevents RNA polymerase (RNAP) promoter by steric...
Haemolysin II, HlyII, is one of several cytotoxic proteins produced by Bacillus cereus, an opportunistic human pathogen that causes food poisoning. The hlyII gene confers haemolytic activity to Escherichia coli cells. Here a new B. cereus gene, hlyIIR, which located immediately downstream and regulates expression, reported. deduced amino acid sequence HlyIIR similar prokaryotic DNA-binding transcriptional regulators the TetR/AcrA family. Measurements levels promoter using fusions...
Abstract Background The majority of experimentally determined crystal structures Type II restriction endonucleases (REases) exhibit a common PD-(D/E)XK fold. Crystal have been also for single representatives two other folds: PLD (R.BfiI) and half-pipe (R.PabI), bioinformatics analyses supported by mutagenesis suggested that some REases belong to the HNH Our previous bioinformatic analysis REase R.Eco29kI shares sequence similarities with one more unrelated nuclease superfamily, GIY-YIG,...
Abstract The convergently transcribed restriction (R) and methylase (M) genes of the Restriction–Modification system Esp1396I are tightly regulated by a controller (C) protein that forms part CR operon. We have mapped transcriptional start sites from each promoter examined regulatory role C.Esp1396I in vivo vitro. C-protein binding at M promoters was analyzed DNA footprinting range biophysical techniques. distal proximal responsible for activation repression, respectively. In contrast, dimer...
Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, CFXyl4) from the GH10 family, while CFXyl3 was GH11 family. The possessed moderate temperature stability a neutral pH optimum. more stable at alkaline values. CFXyl1 CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, xylose, which is typical for GH10. (GH11) CFXyl4 (GH10) formed same xylooligosaccharides, but xylose small amounts....
The article introduces a comparative analysis of storage capacity butter packaged in different ways. Butter with fat fraction 72.5 % was studied monolith sample stabilized structure and fluid state during processing. authors traced the changes sensory, microbiological, physicochemical parameters using standard methods at temperature conditions. When conditions followed State Standard GOST 32261, in-line packaging method proved more effective: it reduced microbiological risks increased...
The Eco29kI restriction-modification (R-M) system consists of two partially overlapping genes, eco29kIR, encoding a restriction endonuclease and eco29kIM, methyltransferase. genes are thought to form an operon with the eco29kIR gene preceding eco29kIM gene. Such organization is expected complicate establishment plasmids containing this R-M in naive hosts, since common logic dictates that methyltransferase should be synthesized first protect DNA from cleavage by endonuclease. Here, we...
A gene encoding a polysaccharide-degrading enzyme was cloned from the genome of bacterium Nocardiopsis halotolerans. Analysis amino acid sequence protein showed presence catalytic domain endo-1,4-β-xylanases GH11 family. The amplified by PCR and ligated into pPic9m vector. recombinant producer based on Pichia pastoria obtained. production enzyme, which we called NhX1, carried out in 10 L fermenter. Enzyme 10.4 g/L with an activity 927 U/mL. Purification NhX1 using Ni-NTA affinity...
Pseudomonas microorganisms are used for bioremediation of soils contaminated with petroleum hydrocarbons. The overall remediation efficiency is largely dependent on the presence macro- and micronutrients. Widely varying concentrations available nitrogen iron (Fe) in were shown to affect residual hydrocarbons course biodegradation. regulatory mechanisms expression hydrocarbon catabolic genes low nitrogen/low conditions remain unclear. catabolism naphthalene, a two-ring polycyclic aromatic...
The origin and evolution of novel biochemical functions remains one the key questions in molecular evolution. We study recently emerged methacrylate reductase function that is thought to have last century reported Geobacter sulfurreducens strain AM-1. report sequence operon coding for flavin-containing (Mrd) tetraheme cytochrome с (Mcc) genome G. Different types signal peptides functionally interlinked proteins Mrd Mcc suggest a possible complex mechanism biogenesis chromoproteids redox...
The strain Pseudomonas putida BS3701 was isolated from soil contaminated with coke by-product waste (Moscow Region, Russian Federation). It is capable of degrading crude oil and polycyclic aromatic hydrocarbons (PAHs). P. genome consists a 6,337,358-bp circular chromosome two plasmids (pBS1141 107,388 bp pBS1142 54,501 bp).
Laccase (EC 1.10.3.2) is one of the most common copper-containing oxidases; it found in many organisms and catalyzes oxidation primarily phenolic compounds by oxygen. Two-domain laccases have unusual thermostability, resistance to inhibitors an alkaline optimum activity. The causes these properties two-domain are poorly understood. A recombinant laccase (SgfSL) was cloned from genome Streptomyces griseoflavus Ac-993, expressed Escherichia coli purified homogeneity. crystals SgfSL belonged...
Restriction-modification (R-M) system Ecl18kI is representative of R-M systems whose coordinated transcription achieved through a separate DNA-binding domain the methyltransferase. M.Ecl18kI recognizes an operator sequence located in noncoding region that separates divergently transcribed R and M genes. Here we show that, contrary to previous predictions, two ecl18kI promoters are not divergent, but actually face one another. The binding its prevents RNA polymerase (RNAP) promoter by steric...
Transcription regulation in bacterial restrictionmodification (RM) systems is an important process, which provides coordinated expression levels of tandem enzymes, DNA methyltransferase (MTase) and restriction endonuclease (RE) protecting cells against penetration alien DNA. The present study focuses on (cytosine-5)-DNA Ecl18kI (M.Ecl18kI), almost identical to SsoII (M.SsoII) terms its structure properties. Each these enzymes inhibits the intrinsic gene activates corresponding RE via binding...
In the genome of Cellulomonas flavigena, two genes that potentially encode endoglucanases - Cfla_2912 and Cfla_2913 were identified. We cloned created Pichia pastoris-based recombinant producers proteins expressed from AOX1 promoter. Each endoglucanase molecules contains a GH6 catalytic domain, CBM2 carbohydrate-binding module, TAT signal peptide. The fermentation was carried out in 10 L fermenter; purified using affinity chromatography. yield comprised 10.3 mg/ml (430 U/ml) for 9 (370...