A5059773502- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Microbial Metabolites in Food Biotechnology
- Amino Acid Enzymes and Metabolism
- Bacterial biofilms and quorum sensing
- RNA modifications and cancer
- Plant Pathogenic Bacteria Studies
- Chromium effects and bioremediation
- Biopolymer Synthesis and Applications
- Bacillus and Francisella bacterial research
- Water Treatment and Disinfection
- Sulfur Compounds in Biology
- Historical Medical Research and Treatments
- Probiotics and Fermented Foods
- Genomics and Chromatin Dynamics
- Metalloenzymes and iron-sulfur proteins
- Folate and B Vitamins Research
- Polyamine Metabolism and Applications
Sirius University of Science and Technology
2022-2024
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
2009-2024
Engelhardt Institute of Molecular Biology
2017-2020
Russian Academy of Sciences
2007-2009
Rutgers, The State University of New Jersey
2009
Significance Hydrogen sulfide (H 2 S) is a highly toxic gas that interferes with cellular respiration; however, at low physiological amounts, it plays an important role in cell signaling. Remarkably, bacteria, endogenously produced H S has been recently recognized as general protective molecule, which renders multiple bacterial species resistant to oxidative stress and various classes of antibiotics. The mechanism this phenomenon remains poorly understood. In paper, we use Escherichia coli...
mRNA vaccines have been shown to be effective in combating the COVID-19 pandemic. The amount of research on use mRNAs as preventive and therapeutic modalities has undergone explosive growth last few years. Nonetheless, issue stability molecules their translation efficiency remains incompletely resolved. These characteristics directly affect expression level a desired protein. Regulatory elements RNA—5′ 3′ untranslated regions (UTRs)—are responsible for efficiency. An optimal combination...
Clustered regularly interspaced short palindromic repeat (CRISPR) is a bacterial immunity system that requires perfect sequence match between the CRISPR cassette spacer and protospacer in invading DNA for exclusion of foreign genetic elements. cassettes are hypervariable, possibly reflecting different exposure strains same species to Here, we determined sequences two Xanthomonas oryzae found one remains sensitive phage Xop411 despite carrying has exactly matching fragment genome. To explain...
Restriction-modification (R-M) system Ecl18kI is representative of R-M systems whose coordinated transcription achieved through a separate DNA-binding domain the methyltransferase. M.Ecl18kI recognizes an operator sequence located in noncoding region that separates divergently transcribed R and M genes. Here we show that, contrary to previous predictions, two ecl18kI promoters are not divergent, but actually face one another. The binding its prevents RNA polymerase (RNAP) promoter by steric...
l-cysteine is the source of all bacterial sulfurous biomolecules. However, cytoplasmic level must be tightly regulated due to its propensity reduce iron and drive damaging Fenton chemistry. It has been proposed that in Escherichia coli component cytochrome bd-I terminal oxidase, CydDC complex, shuttles excessive from cytoplasm periplasm, thereby maintaining redox homeostasis. Here, we provide evidence for an alternative function by demonstrating cydD phenotype, unlike bona fide exporter...
The design of new effective cancer treatment methods is a promising and important research field in translational medicine. Oncolytic viruses can induce immunogenic cell death by activating the body’s immune system to recognize tumor cells. This work presents results for optimizing production recombinant vesicular stomatitis (rVSVs). To ensure assembly viral particles, we developed HEK293TN-T7 line, which stably expresses DNA-dependent RNA polymerase 7 genome transcription, obtained helper...
Abstract Background The majority of experimentally determined crystal structures Type II restriction endonucleases (REases) exhibit a common PD-(D/E)XK fold. Crystal have been also for single representatives two other folds: PLD (R.BfiI) and half-pipe (R.PabI), bioinformatics analyses supported by mutagenesis suggested that some REases belong to the HNH Our previous bioinformatic analysis REase R.Eco29kI shares sequence similarities with one more unrelated nuclease superfamily, GIY-YIG,...
Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, CFXyl4) from the GH10 family, while CFXyl3 was GH11 family. The possessed moderate temperature stability a neutral pH optimum. more stable at alkaline values. CFXyl1 CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, xylose, which is typical for GH10. (GH11) CFXyl4 (GH10) formed same xylooligosaccharides, but xylose small amounts....
A gene encoding a polysaccharide-degrading enzyme was cloned from the genome of bacterium Nocardiopsis halotolerans. Analysis amino acid sequence protein showed presence catalytic domain endo-1,4-β-xylanases GH11 family. The amplified by PCR and ligated into pPic9m vector. recombinant producer based on Pichia pastoria obtained. production enzyme, which we called NhX1, carried out in 10 L fermenter. Enzyme 10.4 g/L with an activity 927 U/mL. Purification NhX1 using Ni-NTA affinity...
The Eco29kI restriction-modification (R-M) system consists of two partially overlapping genes, eco29kIR, encoding a restriction endonuclease and eco29kIM, methyltransferase. genes are thought to form an operon with the eco29kIR gene preceding eco29kIM gene. Such organization is expected complicate establishment plasmids containing this R-M in naive hosts, since common logic dictates that methyltransferase should be synthesized first protect DNA from cleavage by endonuclease. Here, we...
C-proteins control restriction–modification (R–M) systems' genes transcription to ensure sufficient levels of restriction endonuclease allow protection from foreign DNA while avoiding its modification by excess methyltransferase. Here, we characterize regulation in C-protein dependent R–M system Kpn2I. The Kpn2I gene is transcribed a constitutive, weak promoter, which, atypically, independent. (C.Kpn2I) binds upstream the strong methyltransferase promoter and inhibits it, likely preventing...
Restriction-modification (R-M) system Ecl18kI is representative of R-M systems whose coordinated transcription achieved through a separate DNA-binding domain the methyltransferase. M.Ecl18kI recognizes an operator sequence located in noncoding region that separates divergently transcribed R and M genes. Here we show that, contrary to previous predictions, two ecl18kI promoters are not divergent, but actually face one another. The binding its prevents RNA polymerase (RNAP) promoter by steric...
In the genome of Cellulomonas flavigena, two genes that potentially encode endoglucanases - Cfla_2912 and Cfla_2913 were identified. We cloned created Pichia pastoris-based recombinant producers proteins expressed from AOX1 promoter. Each endoglucanase molecules contains a GH6 catalytic domain, CBM2 carbohydrate-binding module, TAT signal peptide. The fermentation was carried out in 10 L fermenter; purified using affinity chromatography. yield comprised 10.3 mg/ml (430 U/ml) for 9 (370...
The widespread use of antibiotics in medicine and agriculture has significantly accelerated the emergence rate bacterial infections showing multiple antibiotic resistance. Since resistance to conventional is developed rather quickly, designing alternative antimicrobial drugs with other mechanisms underlying their effects on bacteria a promising. enzymes possessing bactericidal activity may be one option for such antibacterial agents. study aimed produce combination recombinant protein-based...
Shirokoye ispol'zovaniye antibiotikov v meditsine i sel'skom khozyaystve znachitel'no uskorilo tempy vozniknoveniya bakteriy so mnozhestvennoy ustoychivost'yu k antibiotikam. Poskol'ku traditsionnym antibiotikam dovol'no bystro voznikayet rezistentnost', odnim iz perspektivnykh napravleniy yavlyayetsya razrabotka al'ternativnykh antimikrobnykh preparatov, obladayushchikh inymi mekhanizmami deystviya na bakterii. Fermenty c bakteritsidnym deystviyem mogut byt' variantov takikh...
Gene editing with programmable nucleases opens new perspectives in important practice areas, such as healthcare and agriculture. The most challenging problem for the safe effective therapeutic use of gene technologies is proper delivery expression editors cells tissues different organisms. Virus-based nonviral systems can be used successful editors. Here we have reviewed structural elements DNA- RNA-based vectors methods vitro vivo.
Tekhnologii, lezhashchie v osnove polucheniya sinteticheskih mRNK in vitro, znachitel'no rasshirili vozmozhnosti ih issledovatel'skogo i terapevticheskogo primeneniya. Flagmanskoj oblast'yu primeneniya stala nisha mRNK-vakcin, odnako etot klass terapevticheskih molekul mozhet byt' primenim k gorazdo bolee shirokomu krugu zadach. V processa iskusstvennyh RNK lezhit reakciya fermentativnogo sinteza odnim iz komponentov kotoroj yavlyaetsya ingibitor ribonukleaz. Etot belok neobhodim dlya...
Новый конвергентный промотор As3 системы рестрикции-модификации II типа EcoRI играет ключевую роль в регуляции экспрессии гена эндонуклеазы рестрикции
Objective. To determine whether the genes whose products are annotated as «MBL-fold metallohydrolase superfamily» related to proteins of metallo-β-lactamase group. Materials and Methods. Sequences 7 were analyzed using following resources: ClustalW, IQ-TREE, iTOL. Selection oligonucleotides for real-time PCR was performed Primer-BLAST resource. The level gene expression assessed PCR. MIC MBC measuring cefepime meropenem. double-disc method with EDTA used presence MBL in strain. Results....