A5004277940- Vitamin D Research Studies
- Cancer Cells and Metastasis
- Cancer, Stress, Anesthesia, and Immune Response
- Metabolism, Diabetes, and Cancer
- Immune cells in cancer
- Hormonal Regulation and Hypertension
- Signaling Pathways in Disease
- Phytochemicals and Antioxidant Activities
- Synthesis of Organic Compounds
- Parathyroid Disorders and Treatments
- Galectins and Cancer Biology
- Fibroblast Growth Factor Research
- Cancer Research and Treatments
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Estrogen and related hormone effects
- Erythrocyte Function and Pathophysiology
- Thyroid Disorders and Treatments
- Free Radicals and Antioxidants
- Biological Stains and Phytochemicals
- Bone health and treatments
- Telomeres, Telomerase, and Senescence
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2019-2024
Polish Academy of Sciences
2019-2022
Abstract Background Cancer-associated fibroblasts (CAFs) play an important role in the tumor microenvironment. Despite well-known vitro antitumoral effect of vitamin D 3 (VD ), its impact on breast CAFs is almost unknown. In this study, we analyzed ex vivo effects calcitriol isolated from cancer tissues. Methods were cultured with 1 and 10 nM their phenotype; gene expression, protein secretion assessed. Calcitriol-treated CAFs-conditioned media (CM) used to analyze migration expression MCF-7...
Common food flavonoids: chrysin, apigenin, luteolin, diosmetin, pinocembrin, naringenin, eriodictyol, hesperetin, and their analogues with an additional hydroxyl group at the C-8 position obtained via biotransformation were tested for antioxidant activity using ABTS, DPPH, ferric ion reducing power (FRAP) methods. They also antiproliferative against selected human cancer cell lines—MV-4-11 (biphenotypic B myelomonocytic leukemia), MCF7 (breast carcinoma), LoVo (colon cancer), LoVo/DX...
Abstract Our research found that vitamin D 3 (VD ) treatment increased lung metastasis in mice with 4T1 murine breast cancer (BC). This study aims to investigate the impact of VD on activation tumor-associated macrophages (TAMs) BC. Mice bearing 4T1, E0771, 67NR BC cells, and healthy mice, were fed diets varying contents (100—deficient, 1000—normal, 5000 IU/kg—elevated). Some 1000 100 IU/kg groups received calcitriol. We studied bone characterized TAMs marrow-derived (BMDMs). cells had...
Cancer cell cross-talk with the host endothelium plays a crucial role in metastasis, but underlying mechanisms are still not fully understood. We studied involvement of protein disulphide isomerase A1 (PDIA1) human breast cancer (MCF-7 and MDA-MB-231) adhesion transendothelial migration. For comparison, PDIA1 proliferation, migration, cycle apoptosis was also assessed. Pharmacological inhibitor, bepristat 2a silencing were used to inhibit PDIA1. Inhibition by markedly decreased cells...
The molecular mechanisms of telomerase reverse transcriptase (TERT) upregulation in breast cancer (BC) are complex. We compared genetic variability within TERT and telomere length with the clinical data patients BC. Additionally, we assessed expression TERT, MYC, TP53 SP1 genes BC organoids (3D cell cultures obtained from tissues). observed same correlation blood between TP53. Only was a found MYC MYC. associations genotypes (rs2735940 rs10069690) length. TT genotype rs2735940 have shorter...
Basigin (BSG, CD147) is a multifunctional protein involved in cancer cell survival, mostly by controlling lactate transport through its interaction with monocarboxylate transporters (MCTs) such as MCT1. Previous studies have found that single nucleotide polymorphisms (SNPs) the gene coding for BSG and MCT1, well levels of soluble form (sBSG), are potential biomarkers various diseases. The goal this study was to confirm MCT1 RNA overexpression AML lines, analyse selected BSG/MCT1 genetic...
5-Fluorouracil (5-FU) is an anticancer drug that most frequently used to treat colorectal cancer (CRC) patients, but unfortunately it shows limited efficacy. We recently demonstrated vitamin D analogs (VDAs), particularly tacalcitol (coded as PRI-2191), potentiate its activity in vivo mouse and human CRC model. The purpose of this study was explain the mechanism underlying enhancement 5-FU efficacy by PRI-2191 towards HT-29 cells. showed induces CDKN1A (gene encoding p21Waf1/Cip1) expression...
To analyze if the prometastatic activity of calcitriol (active vitamin D3 metabolite), which was previously observed in a 4T1 breast cancer model, is also found other cancers, and to assess impact various schemes D supply, we used E0771 mouse metastatic 67NR nonmetastatic cells this study. BALB/c C57BL/6 healthy tumor-bearing mice were exposed control (1000 IU), low- (100 high- (5000 IU) diets. Additionally, from day 7 tumor transplantation, 1000 100 IU groups gavaged with (+cal). After 8...
Vitamin D3 and its analogs are known to modulate the activity of fibroblasts under various disease conditions. However, their impact on cancer-associated (CAFs) is yet be fully investigated. The aim this study was characterize CAFs normal (NFs) from lung mice bearing 4T1, 67NR, E0771 cancers healthy fed vitamin-D3-normal (1000 IU), -deficient (100 -supplemented (5000 IU) diets. groups receiving control deficient diets were gavaged with calcitriol (+cal). In 4T1-bearing 100 IU+cal group,...
Experimental data indicate that low-calcemic vitamin D derivatives (VDDs) exhibit anticancer properties, both in vitro and vivo. In our search for a analog as potential agent, we investigated the influence of chirality side chain 1,25-dihydroxyergocalciferol (1,25D2) on their activities. this study, synthesized modified analogs at A-ring, which differed from one another absolute configuration C-24, namely (24S)- (24R)-1,25-dihydroxy-19-nor-20a-homo-ergocalciferols (PRI-5105 PRI-5106,...