Alternative splicing (AS) is a key post-transcriptional regulatory mechanism, yet little information known about its roles in fruit crops. Here, AS was globally analyzed the wild strawberry Fragaria vesca genome with RNA-seq data derived from different stages of development. The landscape characterized and compared between single-molecule, real-time (SMRT) Illumina platform. While SMRT has lower sequencing depth, it identifies more genes undergoing (57.67% detected multiexon genes) when...

Auxin response factors (ARFs) are an important family of proteins in auxin-mediated response, with key roles various physiological and biochemical processes. To date, a genome-wide overview the ARF gene citrus was not available. A systematic analysis this begun by carrying out search for homologs ARFs. total 19 nonredundant genes (CiARF) were found validated from sweet orange. comprehensive CiARFs undertaken, including structures, phylogenetic analysis, chromosome locations, conserved motifs...

Microsatellites or simple sequence repeats (SSRs) are one of the most popular sources genetic markers and play a significant role in plant genetics breeding. In this study, we identified citrus SSRs genome Clementine mandarin analyzed their frequency distribution different genomic regions. A total 80,708 were detected with an overall density 268 SSRs/Mb. While di-nucleotide frequent microsatellites DNA sequence, tetra-nucleotides, which had more repeat units than any other SSR types, highest...

Abstract Beta-amylase (BAM) plays an important role in plant resistance to cold stress. However, the specific of BAM gene freezing tolerance is poorly understood. In this study, we demonstrated that a cold-responsive module was involved kiwifruit. module, expression AaBAM3.1 , which encodes functional protein, induced by -overexpressing kiwifruit lines showed increased tolerance, and heterologous overexpression Arabidopsis thaliana resulted similar phenotype. The results promoter GUS...

MYB family genes are widely distributed in plants and comprise one of the largest transcription factors involved various developmental processes defense responses plants. To date, few little expression profiling have been reported for citrus. Here, we describe classify 177 members sweet orange gene (CsMYB) terms their genomic structures similarity to putative Arabidopsis orthologs. According these analyses, CsMYBs were categorized into four groups (4R-MYB, 3R-MYB, 2R-MYB 1R-MYB). Gene...

Advanced techniques capable of early, rapid, and nondestructive detection the impacts drought on fruit tree measurement underlying photosynthetic traits a large scale are necessary to meet challenges precision farming full prediction yield increases. We tested application hyperspectral reflectance as high-throughput phenotyping approach for early identification water stress rapid assessment leaf in citrus trees by conducting greenhouse experiment. To this end, CO2 assimilation rate (Pn),...

Cold stress poses a serious treat to cultivated kiwifruit since this plant generally has weak ability tolerate freezing tolerance temperatures. Surprisingly, however, the underlying mechanism of kiwifruit’s remains largely unexplored and unknown, especially regarding key pathways involved in conferring trait. Here, we studied metabolome transcriptome profiles freezing-tolerant genotype KL ( Actinidia arguta ) freezing-sensitive RB A. ), identify main important metabolites related their...

WUSCHEL-related homeobox (WOX) transcription factors (TFs) are well known for their role in plant development but rarely studied citrus. In this study, we identified 11 putative genes from the sweet orange genome and divided citrus WOX into three clades (modern/WUSCHEL(WUS), intermediate, ancient). Subsequently, performed syntenic relationship, intron-exon organization, motif composition, cis-element analysis. Co-expression analysis based on RNA-seq tissue-specific expression patterns...

Abstract Background Kiwifruit ( Actinidia Lindl.) is considered an important fruit species worldwide. Due to its temperate origin, this highly vulnerable freezing injury while under low-temperature stress. To obtain further knowledge of the mechanism underlying tolerance, we carried out a hybrid transcriptome analysis two A. arguta Actinidi ) genotypes, KL and RB, whose tolerance high low, respectively. Both genotypes were subjected − 25 °C for 0 h, 1 4 h. Results SMRT (single-molecule...

Actinidia valvata possesses waterlogging tolerance; however, the mechanisms underlying this trait are poorly characterized. Here, we performed a transcriptome analysis by combining single-molecule real-time (SMRT) sequencing and Illumina RNA investigated physiological responses of roots KR5 (A. valvata, tolerant genotype) after 0, 12, 24 72 h stress. responded to stress mainly via carbohydrate free amino acids metabolism reactive oxygen species (ROS) scavenging pathways....

Waterlogging stress is one of the greatest environmental threats to kiwifruit growth and development. ERF-VII proteins have been demonstrated play pivotal roles in regulating plant tolerance waterlogging. Nevertheless, genome-wide role waterlogging remains unclear. Here, we report function regulatory network an transcription factor located nucleus, AvERF73, tolerance. Overexpression AvERF73 Arabidopsis thaliana A. chinensis cv. Hongyang enhanced transgenic plants. Furthermore, performed...

Flowering is a complex developmental mechanism and essential for successful reproduction in plants. Complex regulatory networks transform vegetative shoot apical meristems into inflorescence meristems. Further, floral transition to bud outgrowth flowering. Floral pathways are independently involved flowering, most of what we know about genetic regulation comes from model Despite the advancements plant development biology, understanding molecular mechanisms signals horticultural plants...

After several years in the juvenile phase, trees undergo flowering transition to become mature (florally competent) trees. This depends on balanced expression of a complex network genes that is regulated by both endogenous and environmental factors. However, relatively little known about molecular processes regulating woody plants compared with herbaceous plants.

Abstract Long non-coding RNAs (lncRNAs) have been demonstrated to play critical regulatory roles in post-transcriptional and transcriptional regulation Arabidopsis . However, lncRNAs their functional remain poorly characterized woody plants, including citrus. To identify investigate role citrus flowering, paired-end strand-specific RNA sequencing was performed for precocious trifoliate orange its wild-type counterpart. A total of 6,584 potential were identified, 51.6% which from intergenic...

Kiwifruit bacterial canker is a devastating disease threatening kiwifruit production. To clarify the defense mechanism in response to Pseudomonas syringae pv. actinidiae (Psa), we observed phenotypic changes resistant Huate (HT) and susceptible Hongyang (HY) varieties at 0, 12, 24, 48, 96, 144 hour after inoculation (hai) with Psa. Brown lesions appeared areas 12 hai HY shoots, lesion length gradually increased from 24 h. In contrast, no were found HT shoots any time points. Furthermore,...