A5081931568- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Radiopharmaceutical Chemistry and Applications
- Natural Antidiabetic Agents Studies
- Molecular Sensors and Ion Detection
- Metabolism, Diabetes, and Cancer
- Wastewater Treatment and Nitrogen Removal
- Genomics and Chromatin Dynamics
- Carbohydrate Chemistry and Synthesis
- Inflammatory Bowel Disease
- Chemical Synthesis and Analysis
- Peptidase Inhibition and Analysis
- Electrochemical Analysis and Applications
- Chemical Reaction Mechanisms
- Drug Solubulity and Delivery Systems
- Carbon Dioxide Capture Technologies
- Pregnancy and Medication Impact
- Catalysts for Methane Reforming
- Thermodynamic properties of mixtures
- Fluorine in Organic Chemistry
- Pneumocystis jirovecii pneumonia detection and treatment
- Echinoderm biology and ecology
- Microbial Fuel Cells and Bioremediation
- Pharmaceutical and Antibiotic Environmental Impacts
- Chemical Looping and Thermochemical Processes
Beckman Research Institute
2021-2025
City of Hope
2021-2025
University of Southern California
2018-2024
Sharif University of Technology
2016-2020
Research Network (United States)
2017-2020
Targeting transcription replication conflicts, a major source of endogenous DNA double-stranded breaks and genomic instability could have important anticancer therapeutic implications. Proliferating cell nuclear antigen (PCNA) is critical to repair processes. Through rational drug design approach, we identified small molecule PCNA inhibitor, AOH1996, which selectively kills cancer cells. AOH1996 enhances the interaction between largest subunit RNA polymerase II, RPB1, dissociates from...
Abstract Proliferating cell nuclear antigen (PCNA) plays a critical role in at least nine distinct cellular pathways, including DNA replication and repair, transcription, cycle control, apoptosis induction, transcription-replication conflict resolution, immune surveillance. Tumor cells express an acidic isoform of this protein (caPCNA), whereas non-cancer only basic (nmPCNA). The absence the caPCNA makes it ideal target for selectively killing cancer while preserving viability cells. To end,...
Research Article| April 07 2017 Efficient removal of some anionic dyes from aqueous solution using a polymer-coated magnetic nano-adsorbent Armin Kiani; Kiani 1Research Center for Analytical Chemistry, KAVA Institute, Tehran, Iran Search other works by this author on: This Site PubMed Google Scholar Pouya Haratipour; Haratipour 2Department Sharif University Technology, Mazaher Ahmadi; Ahmadi 3Young Researchers and Elite Club, Hamedan Branch, Islamic Azad University, Hamedan, E-mail:...
We examine the DNA polymerase β (pol β) transition state (TS) from a leaving group pre-steady-state kinetics perspective by measuring rate of incorporation dNTPs and corresponding novel β,γ-CXY-dNTP analogues, including individual β,γ-CHF -CHCl diastereomers with defined stereochemistry at bridging carbon, during formation right (R) wrong (W) base pairs. Brønsted plots log kpol versus pKa4 bisphosphonic acids are used to interrogate effects identity, dNTP analogue basicity, precise...
Complex bacterial glycoconjugates drive interactions between pathogens, symbionts, and their human hosts. Glycoconjugate biosynthesis is initiated at the membrane interface by phosphoglycosyl transferases (PGTs), which catalyze transfer of a phosphosugar from soluble uridine diphosphosugar (UDP-sugar) substrate to membrane-bound polyprenol-phosphate (Pren-P). The two distinct superfamilies PGT enzymes (polytopic monotopic) show striking differences in structure mechanism. We designed...
Deoxynucleotide misincorporation efficiencies can span a wide 10
DNA polymerase β (pol β) selects the correct deoxyribonucleoside triphosphate for incorporation into polymer. Mistakes made by pol lead to mutations, some of which occur within specific sequence contexts generate mutation hotspots. The adenomatous polyposis coli (APC) gene is mutated in colorectal carcinomas but underlying mechanism not fully understood. In previous work, we demonstrated that a somatic colon cancer variant β, K289M, misincorporates deoxynucleotides at significantly increased...
Nucleoside 5′-triphosphate (dNTP) analogues in which the β,γ-oxygen is mimicked by a CXY group (β,γ-CXY-dNTPs) have provided information about DNA polymerase catalysis and fidelity. Definition of stereochemistry important to elucidate precise binding modes. We previously reported (R)- (S)-β,γ-CHX-dGTP diastereomers (X = F, Cl), prepared via P,C-dimorpholinamide CHCl (6a, 6b) CHF (7a, 7b) bisphosphonates (BPs) equipped with an (R)-mandelic acid as chiral auxiliary, final deprotection using...
Individual diastereomers of CXY bisphosphonate analogues dNTPs or NTPs are useful chemical stereoprobes to investigate interactions within the chiral active site environment enzymes such as polymerases and kinases. We previously reported synthetic access β,γ-CHX-dGTPs (X = F Cl) via a synthon with an (R)-methyl mandelate auxiliary have extended this approach dTTP dATP analogues. As removal by (Pd/C) hydrogenolysis is incompatible cytosine heterocycle also X Br, we now designed synthons using...
A recently described DNA polymerase ribozyme, obtained by in vitro evolution, provides the opportunity to investigate mechanistic features of RNA catalysis using methods that previously had only been applied proteins. Insight can be gained into transition state polymerization reaction studying behavior various β,γ-bridging substituted methylene (CXY; X, Y = H, halo, methyl) or imido (NH) dNTP analogues differ with regard pKa4 bisphosphonate imidodiphosphate leaving group. The apparent rate...
Abstract Proliferating cell nuclear antigen (PCNA) is a proliferation biomarker in variety of human tumors and has critical cellular functions DNA replication repair. We previously identified unique cancer-associated isoform the protein, termed caPCNA, which allows for structure-based drug discovery studies to develop inhibitors selective therapeutic targeting cancer cells. Distinct strategies have been used caPCNA agents, includes peptide small molecule-based compounds, although success...