A5064982518- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- Alzheimer's disease research and treatments
- Retinal Development and Disorders
- Chemical Synthesis and Analysis
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Liver Disease Diagnosis and Treatment
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Pancreatic function and diabetes
- Calcium signaling and nucleotide metabolism
- Photoreceptor and optogenetics research
- Cannabis and Cannabinoid Research
- Cholinesterase and Neurodegenerative Diseases
- Synthesis and Biological Evaluation
- Pharmacological Receptor Mechanisms and Effects
- Phenothiazines and Benzothiazines Synthesis and Activities
- Plant Stress Responses and Tolerance
- Diet, Metabolism, and Disease
Aptuit (Italy)
2016-2022
University of Padua
2012-2019
Mutations in amyloid precursor protein (APP), and presenilin-1 presenilin-2 (PS1 PS2) have causally been implicated Familial Alzheimer's Disease (FAD), but the mechanistic link between mutations early onset of neurodegeneration is still debated. Although no consensus has yet reached, most data suggest that both FAD-linked PS mutants endogenous PSs are involved cellular Ca2+ homeostasis. We here investigated subcellular handling primary neuronal cultures acute brain slices from wild type...
Abstract In planta, very limited information is available about how the endoplasmic reticulum (ER) contributes to cellular Ca2+ dynamics and homeostasis. Here, we report generation of an ER-targeted Cameleon reporter protein suitable for analysis accumulation in lumen ER plant cells. Using stably transformed Arabidopsis (Arabidopsis thaliana) plants expressing this protein, observed a transiently enhanced response stimuli inducing cytosolic rises root tip all experimental conditions, were...
An imbalance in Ca2+ homeostasis represents an early event the pathogenesis of Alzheimer's disease (AD). Presenilin-1 and -2 (PS1 PS2) mutations, major cause familial AD (FAD), have been extensively associated with alterations different signaling pathways, particular those handled by storage compartments. However, FAD-PSs effect on organelles content is still debated mechanism action mutant proteins unclear. To fulfil need a direct investigation intracellular stores dynamics, we here present...
The Golgi apparatus (GA) is a dynamic intracellular Ca2+ store endowed with complex homeostatic mechanisms in part distinct from those of the endoplasmic reticulum (ER). We describe generation novel fluorescent probe selectively targeted to medial-Golgi. demonstrate that medial-Golgi: (i) accumulation takes advantage two pumps, sarco/endoplasmic ATPase and secretory pathway ATPase1; (ii) activation IP3 or ryanodine receptors causes release, while no functional two-pore channel was found;...
Calcium ion (Ca2+) is a ubiquitous intracellular messenger and changes in its concentration impact on nearly every aspect of cell life. Endoplasmic reticulum (ER) represents the major Ca2+ store free ([Ca2+]) within lumen ([Ca2+]ER) can reach levels higher than 1 mM. Several genetically-encoded ER-targeted sensors have been developed over last years. However, most them are non-ratiometric and, thus, their signal difficult to calibrate live cells affected by shifts focal plane artifactual...
Mammalian INDY (mINDY, NaCT, gene symbol SLC13A5) is a potential target for the treatment of metabolically associated fatty liver disease (MAFLD). This study evaluated effects selective, cross-species active, non-competitive, non-substrate-like inhibitor NaCT. First, small molecule ETG-5773 was citrate and succinate uptake acid synthesis in cell lines expressing both human NaCT mouse Nact. Once its suitability established, diet-induced obesity (DIO) model. DIO mice treated with 15 mg/kg...
A novel series of 1,2,4-triazolyl 5-azaspiro[2.4]heptanes with high affinity and selectivity at the dopamine (DA) D3 receptor (D3R) is described. Some these compounds also have over hERG channel were characterized respect to their pharmacokinetic properties both in vitro vivo during lead identification early optimization phases. few derivatives overall favorable developability characteristics selected for further late studies.
Calcium (Ca2+) signalling dysfunction is extensively reported in Familial Alzheimer's Disease (FAD). Presenilins (PSs) play a key role on this aspect, although their mechanism of action still debated. FAD-PSs are mainly to cause exaggerated Ca2+ release but exact effect intracellular stores unclear. To directly evaluate effect, one needs investigate the Endoplasmic Reticulum (ER), main store. Other organelles also involved, though. In particular, Golgi apparatus (GA), hub for protein...