A5103229378- Connexins and lens biology
- Lipid Membrane Structure and Behavior
- Heat shock proteins research
- Urinary Bladder and Prostate Research
- Urological Disorders and Treatments
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Intraocular Surgery and Lenses
- Photoreceptor and optogenetics research
- Protein Structure and Dynamics
- Biochemical effects in animals
- Autophagy in Disease and Therapy
- Ocular Surface and Contact Lens
- Corneal surgery and disorders
- Retinal Development and Disorders
- Aldose Reductase and Taurine
- Cardiomyopathy and Myosin Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Erythrocyte Function and Pathophysiology
- Advanced MRI Techniques and Applications
- Pelvic floor disorders treatments
- Advanced Electron Microscopy Techniques and Applications
- Urinary Tract Infections Management
- Liquid Crystal Research Advancements
- RNA and protein synthesis mechanisms
University of North Carolina at Chapel Hill
2013-2023
Duke University
1978-2013
University of North Carolina Health Care
2005-2009
Institute of Cell Biology and Neurobiology
1982-2001
Argonne National Laboratory
1992-1994
Sorbonne Université
1992-1994
Australian National University
1992-1994
Advisory Board Company (United States)
1992-1994
University College London
1992-1994
University of Tennessee System
1994
Junctions between fiber cells of bovine lenses have been isolated in milligram quantities, without using detergents or proteases. The structure the junctions has studied by thin-section, negative-stain, and freeze-fracture electron microscopy x-ray diffraction. are large most often an undulating surface topology as determined thin sectioning freeze-fracture. These undulations resemble tongue-and-groove interdigitations lens previously seen others (D. H. Dickson G. W. Crock, 1972, Invest....
A polycrystalline phospholipid monolayer self-assembled at the surface of an air microbubble in aqueous solution represents a novel material structure: essence, solid shell wax with micrometer-scale dimensions and thickness only single molecule. Micropipet manipulation these microparticles revealed dependence mechanical properties lipid shells, specifically, yield shear viscosity, on composition, grain microstructure, thermal processing material, particular cooling rate shells from melt....
Purified lac permease, the 46.5-kDa product of Y gene that catalyzes lactose/H+ symport, or purified cytochrome o, a terminal oxidase Escherichia coli respiratory chain composed four subunits with composite molecular mass 140 kDa, was reconstituted into proteoliposomes individually in combination. The preparations were then examined by freeze-fracture electron microscopy employing conventional platinum/carbon replicas means new technique using thin tantalum replicas. In nonenergized...
Two methods of freezing samples for freeze-fracture electron microscopy have been compared using X-ray diffraction and results from lipid-water model systems. Perturbations the molecular organization hydrocarbon chains extent ice crystal formation evaluated lamellar phases egg lecithin containing 16% water lecithin-phosphatidylinositol 55% water, both freeze quenched in liquid Freon-22 near its melting temperature (113 K). Very thin sandwiched between copper sheets separated by an microscope...
Freeze-fracture electron microscopy has been used to examine defect structures such as confocal domains, screw dislocations, disclinations, and walls in lamellar phospholipid phases containing known amounts of water (from about 5 65% by weight). In a concurrent study (Costello Gulik-Krzywicki 1976) all samples were examined X-ray diffraction before after freeze-quenching ensure that the freezing process had not induced phase transition. Most prominent structures, especially have observed...
Defects in clearing apoptotic debris disrupt tissue and immunological homeostasis, leading to autoimmune inflammatory diseases. Herein, we report that macrophages from lupus-prone MRL/lpr mice have impaired lysosomal maturation, resulting heightened ROS production attenuated acidification. Impaired maturation diminishes the ability of lysosomes degrade contained within IgG-immune complexes (IgG-ICs) promotes recycling accumulation nuclear self-antigens at membrane 72 h after internalization....
We report the successful application of freeze fracture technique for obtaining transmission-electron-microscope pictures a thermotropic cholesteric material and its blue phase. It is shown that under rapid quenching these phases supercool conserve their structure at liquid-${\mathrm{N}}_{2}$ temperature. The micrographs obtained confirm body-centered-cubic symmetry phase I.
FYCO1 (FYVE and coiled-coil domain containing 1) is an adaptor protein, expressed ubiquitously required for microtubule-dependent, plus-end-directed transport of macroautophagic/autophagic vesicles. We have previously shown that loss-of-function mutations in cause cataracts with no other ocular and/or extra-ocular phenotype. Here, we show fyco1 homozygous knockout (fyco1−/−) mice recapitulate the cataract phenotype consistent a critical role autophagy lens morphogenesis. Transcriptome...
Fragments of intact rat, pig and bovine lenses isolated membranes from were examined by freeze-fracture-etch electron microscopy employing samples ultra-rapidly frozen in the absence fixatives or cryoprotectants. Complementary replicas outer cortex clearly display small patches square arrays (6.6 nm repeat) as structures distinct gap junctions same membranes. In appear single where extracellular space is not thinned, whereas junctions, other tissues, only occur greatly attenuated. Square...
To employ Mie scattering theory to predict the light-scattering from micrometer-sized particles surrounded by lipid shells, called multilamellar bodies (MLBs), reported in human age-related nuclear cataracts.Mie is applicable randomly distributed spherical and globular separated distances much greater than wavelength of incident light. With an assumed refractive index 1.40 for cytoplasm, particle indices 1.33 1.58 were used calculate efficiencies radii 0.05 3 microm light with wavelengths...
Proteoliposomes reconstituted with purified lac carrier protein from Escherichia coli were ultra-rapidly frozen and examined by freeze-fracture-etch electron microscopy. The proteoliposomes are greater than 95% unilamellar, the majority 30-150 nm in diameter. Fracture faces of (at a protein:lipid molecular ratio about 1:2500) display 7.0-nm diameter globular intramembrane particles uniformly distributed on convex concave surfaces. Calculations particle composition suggest that each probably...