A5006555835- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Nutrition, Genetics, and Disease
- Vitamin D Research Studies
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Bacterial Genetics and Biotechnology
- Heavy Metals in Plants
- Effects and risks of endocrine disrupting chemicals
- Complementary and Alternative Medicine Studies
- RNA and protein synthesis mechanisms
- Metabolomics and Mass Spectrometry Studies
- Folate and B Vitamins Research
- Protein Hydrolysis and Bioactive Peptides
- Metabolism and Genetic Disorders
- Identification and Quantification in Food
- Pharmacological Effects and Assays
- Tea Polyphenols and Effects
- Vitamin C and Antioxidants Research
- Consumer Attitudes and Food Labeling
- Neuroscience and Neuropharmacology Research
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- Nanopore and Nanochannel Transport Studies
- Urological Disorders and Treatments
National Institutes of Health
2015-2025
Office of Dietary Supplements
2016-2025
National Institute of Diabetes and Digestive and Kidney Diseases
2013-2020
Yale University
2020
Harvard University
2020
Rockefeller University
2020
National Sanitation Foundation International
2016
Pfizer (United States)
2016
Covance (United States)
2016
Herbalife (Australia)
2016
Despite extensive characterization of the mu-opioid receptor (MOR), biochemical properties isolated remain unclear. In light recent reports, we proposed that monomeric form MOR can activate G proteins and be subject to allosteric regulation. A fused yellow fluorescent protein (YMOR) was constructed expressed in insect cells. YMOR binds ligands with high affinity, displays agonist-stimulated [(35)S]guanosine 5'-(gamma-thio)triphosphate binding Galpha(i), is allosterically regulated by coupled...
While great interest in health effects of natural product (NP) including dietary supplements and foods persists, promising preclinical NP research is not consistently translating into actionable clinical trial (CT) outcomes. Generally considered the gold standard for assessing safety efficacy, CTs, especially phase III are costly require rigorous planning to optimize value information obtained. More effective bridging from CT was goal a September, 2018 transdisciplinary workshop....
Voltage-dependent anion channel (VDAC) is the major pathway for transport of ions and metabolites across mitochondrial outer membrane. Among three known mammalian VDAC isoforms, VDAC3 least characterized, but unique functional roles have been proposed in cellular animal models. Yet, a high-sequence similarity between VDAC1 indicative similar pore-forming structure. Here, we conclusively show that forms stable, highly conductive voltage-gated channels that, much like VDAC1, are weakly...
Abstract Commutability is where the measurement response for a reference material (RM) same as an individual patient sample with concentration of analyte measured using two or more systems. Assessment commutability essential when RM used in calibration hierarchy to ensure that clinical measurements are comparable across different procedures and at times. The three new Standard Reference Materials ® (SRMs) determining serum total 25-hydroxyvitamin D [25(OH)D], defined sum 2 [25(OH)D ] 3 ],...
In this study we used correlative light, scanning, and transmission (freeze-etch) electron microscopy to characterize lens structure in normal mice compare it with that deficient the major intrinsic protein (MIP) of fiber cells. Grossly, wild-type lenses were transparent had typical Y sutures at all ages examined. These fibers uniform shape (hexagonal cross section) arranged ordered concentric growth shells radial cell columns. addition, these opposite end curvature lateral interdigitations...
Nearly all mitochondrial proteins are coded by the nuclear genome and must be transported into mitochondria translocase of outer membrane complex. Tom40 is central subunit complex forms a pore in membrane. To date, mechanism it utilizes for protein transport remains unclear. predicted to comprise membrane-spanning β-barrel domain with conserved α-helical domains at both N C termini. investigate function, including role N- C-terminal domains, recombinant from yeast Candida glabrata, truncated...
Mechanistic, clinical, and epidemiological research relevant to dietary supplements (DS) is supported by the U.S. National Institutes of Health. The Office Dietary Supplements Center for Complementary Integrative Health promote development appropriate use rigorous comprehensive DS analyses which are critical reproducibility, particularly when investigational include chemically complex natural products with unclear mechanisms action.
Abstract Background The National Institute of Standards and Technology (NIST) has produced over 40 botanical dietary supplement Standard Reference Materials (SRMs) reference materials (RMs) with values assigned for chemical markers and/or active compounds. Although environmental accumulation or inadvertent introduction toxic elements (arsenic, cadmium, lead, mercury) is a potential source exposure in products, the majority SRMs/RMs do not have four major elements. Objective To determine As,...