A5013951018- Nutrition, Genetics, and Disease
- Nutritional Studies and Diet
- Digestive system and related health
- Cancer, Lipids, and Metabolism
- Angiogenesis and VEGF in Cancer
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Consumer Attitudes and Food Labeling
- Fatty Acid Research and Health
- Cancer, Hypoxia, and Metabolism
- Liver physiology and pathology
- Bone and Dental Protein Studies
- Nutrition and Health in Aging
- Genomics, phytochemicals, and oxidative stress
- Health Sciences Research and Education
- Probiotics and Fermented Foods
- Metabolomics and Mass Spectrometry Studies
- Trace Elements in Health
- Folate and B Vitamins Research
- Cancer Research and Treatments
- Helicobacter pylori-related gastroenterology studies
- Estrogen and related hormone effects
- Cell Adhesion Molecules Research
- Clinical Nutrition and Gastroenterology
- Protease and Inhibitor Mechanisms
National Cancer Institute
2014-2024
National Institutes of Health
2007-2022
Center for Cancer Research
2008-2022
National Cancer Institute
2019
Yale University
1995-2001
Columbia University
1999-2001
VA Connecticut Healthcare System
1996-1998
Yale New Haven Health System
1997
The Ohio State University
1994-1996
Collagen I, the most abundant protein in humans, is ubiquitous solid tumors where it provides a rich source of exploitable metabolic fuel for cancer cells. While tumor cells were unable to exploit collagen directly, here we show they can usurp byproducts collagen-consuming tumor-associated stroma. Using genetically engineered mouse models, discovered that growth depends upon binding and uptake mediated by TEM8/ANTXR1 cell surface Tumor-associated stromal processed into glutamine, which was...
Extracellular matrix regulation of intestinal epithelial differentiation may affect development, during migration to villus tips, healing, inflammatory bowel disease, and malignant transformation. Cell culture studies biology also depend on the substrate used. We evaluated effects proliferation in human Caco-2 cells, a model for differentiation. Proliferation, brush border enzyme specific activity, spreading were compared cells cultured tissue plastic with interstitial collagen I basement...
High intakes of dietary fiber or resistant starches have been associated with a lower incidence colon cancers. Because short-chain fatty acids (SCFA) such as butyrate are produced in the colonic lumen by bacterial fermentation fibers and starches, we hypothesized that SCFA may inhibit development invasive human To test this hypothesis, primary colonocytes were isolated from fresh surgical specimens treated 0.01 mol/L acetate, propionate butyrate; cell invasion, adhesion, F-actin...
Abstract The concept of green chemoprevention was introduced in 2012 by Drs. Jed Fahey and Thomas Kensler as whole-plant foods and/or extract-based interventions demonstrating cancer prevention activity. Refining concepts research proof-of-principle approaches are highlighted within this review. Early included extensively investigated whole foods, including broccoli sprouts black raspberries showing dose–responsive effects across a range activities both animals humans with minimal or no...
Fermentation of dietary fiber within the colonic lumen yields short chain fatty acids (SCFA) such as butyrate, which may modulate mucosal biology and inhibit development a malignant phenotype. However, different fibers yield varying proportions various SCFA. We studied effects three most common SCFA, acetate, propionate, on proliferation, adhesion, motility human intestinal Caco-2 cell line, well these SCFA alkaline phosphatase dipeptidyl dipeptidase specific activity (common laboratory...
Short-chain fatty acids (SCFAs) butyrate, propionate, and acetate produced during fiber fermentation promote colonic differentiation can reverse or suppress neoplastic progression. We sought to identify candidate genes responsible for SCFA activity on colonocytes compare the relative activities of independent SCFAs. cDNA was generated from polyA+ mRNA isolated control Caco-2 cells treated with equimolar acetate. GeneCalling, a restriction-based differential RNA expression platform linked DNA...
Thrombospondin 1 is a glycoprotein that regulates cellular phenotype through interactions with its receptors and extracellular matrix-binding partners. locally angiogenesis inflammatory responses contribute to colorectal carcinogenesis in Apc(Min/+) mice. The ability of thrombospondin regulate cells tissues variety stresses suggested loss may also have broader systemic effects on metabolism modulate carcinogenesis. Apc(Min/+):Thbs1(-/-) mice exhibited decreased survival higher tumor...
Thrombospondin-1 (TSP1) is a matricellular protein with many important roles in mediating carcinogenesis, fibrosis, leukocyte recruitment, and metabolism. We have previously shown role of diet the absence TSP1 liver metabolism context colorectal cancer model. However, metabolic implications regulation by are currently understudied. Therefore Discrete correlation summation (DCS) was used to re-interrogate data determine alterations deficiency liver, providing new insights into injury...
Background/Aims: Cell-matrix interactions influence intestinal epithelial biology, but the whether specific integrin heterodimers exert different effects is unclear. Methods: We used functional antibodies to investigate of α2, α3, α5, and α6 subunits on proliferation differentiation human Caco-2 cells laminin. Cells seeded onto laminin-coated inserts in defibronectinized medium were treated with or normal IgG for 72 hrs proliferation, alkaline phosphatase dipeptidyl dipeptidase activity...