A5001970352- Photoreceptor and optogenetics research
- Analytical Chemistry and Chromatography
- Eicosanoids and Hypertension Pharmacology
- Pharmacogenetics and Drug Metabolism
- Retinal Development and Disorders
- Metabolism and Genetic Disorders
- Receptor Mechanisms and Signaling
- Diet and metabolism studies
- Circadian rhythm and melatonin
- Neurobiology and Insect Physiology Research
- Carbohydrate Chemistry and Synthesis
- Analytical Methods in Pharmaceuticals
- Glaucoma and retinal disorders
- bioluminescence and chemiluminescence research
- Biopolymer Synthesis and Applications
- Synthesis and biological activity
- Inflammatory mediators and NSAID effects
- Glycosylation and Glycoproteins Research
- Synthesis and Characterization of Heterocyclic Compounds
- Neuroscience and Neuropharmacology Research
- Drug-Induced Hepatotoxicity and Protection
- Chemical Reactions and Isotopes
- Chemical Synthesis and Analysis
- Biochemical Acid Research Studies
- Molecular Communication and Nanonetworks
Kobe University
2021-2025
Optica
2024-2025
Tosoh (Japan)
2025
Institute for Molecular Science
2012-2023
Japan Science and Technology Agency
2005-2023
Osaka City University
2006-2019
The Graduate University for Advanced Studies, SOKENDAI
2014-2018
Oregon Health & Science University
2009-2013
Kyoto University
2004-2012
National Institutes of Natural Sciences
2012
Pharmacological properties of morphine-3-glucuronide and morphine-6-glucuronide were studied in mice compared with those morphine. 1) The analgesic effect was 3 to 4 times as potent approximately 2 long duration that morphine when injected subcutaneously. Morphine-3-glucuronide, however, showed no even a dose 27.6mg/kg. 2) about 45 morphine, intracerebrally mice, but had effect. 3) After intraperitoneal injection rats, only conjugated detected the brain by chromatographic examination not...
To investigate whether the aqueous levels of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) are correlated to vitreous these substances severity macular oedema in branch retinal vein occlusion (BRVO).Aqueous samples were obtained during cataract surgery from 24 patients (24 eyes) with BRVO. The VEGF IL-6 humour, fluid, plasma determined by enzyme-linked immunosorbent assay. degree ischaemia was evaluated terms area capillary nonperfusion using Scion Image. OCT.The level...
Most opsins selectively bind 11-cis retinal as a chromophore to form photosensitive pigment, which underlies various physiological functions, such vision and circadian photoentrainment. Recently, opsin 3 (Opn3), originally called encephalopsin or panopsin, its homologs were identified in tissues including brain, eye, liver both vertebrates invertebrates, human. Because Opn3s are mainly expressed that not considered contain sufficient amounts of pigments, the photopigment formation ability...
Light sensing starts with phototransduction in photoreceptor cells. The cascade has diverged different species, such as those mediated by transducin vertebrate rods and cones, G q -type protein insect molluscan rhabdomeric-type visual cells photosensitive retinal ganglion cells, o scallop ciliary-type Here, we investigated the of a prebilaterian box jellyfish, most basal animal having eyes containing lens similar to eyes, examine similarity at molecular level obtain an implication origin...
The principal eyes of jumping spiders have a unique retina with four tiered photoreceptor layers, on each which light different wavelengths is focused by lens appreciable chromatic aberration. We found that all photoreceptors in both the deepest and second-deepest layers contain green-sensitive visual pigment, although green only layer. This mismatch indicates layer always receives defocused images, depth information scene optical theory. Behavioral experiments revealed perception spider was...
1. ρ-Nitrophenyl β-D-glucosiduronic acid is proposed as a substrate for the assay of hydrolytic activity β-glucuronidase. The liberated p-nitrophenol measured photocolorimetrically in alkaline solution. 2. chemical synthesis p-nitropenyl β-D-glucopyranosiduronic was accomplished by hydrolysis methyl (p-nitrophenyl 2, 3, 4-tri-O-acetyl-β-D-glucopyranosid) uronate which prepared condensation 1-bromo-2, 4-tri-O-acetyl-α-D-glucopyranuronate and using acetonitrile freshly silver oxide. 3....
Ciliary opsins (c-opsin) have been identified not only in vertebrates but also invertebrates. An invertebrate ciliary opsin was recently the fan worm Acromegalomma interruptum (formerly named Megalomma interrupta); however, its spectral and signaling characteristics are unknown. In present study, we characterized properties light-induced cellular of (Acromegalomma (AcrInvC-opsin)). AcrInvC-opsin showed an absorption maximum at 464 nm, upon blue light absorption, spectrum red-shifted by...
Two type 2A protein phosphatases, phosphatases I (Mr = 180,000) and III 177,000), were purified to near homogeneity from human erythrocyte cytosol. Phosphatase was composed of alpha (34 kDa), beta (63 delta (74 kDa) subunits in a ratio 1:1:1. comprised alpha, beta, gamma (53 the same ratio. Heparin-Sepharose column chromatography converted most phosphatase 20% into 1 which indistinguishable IV (Usui, H., Kinohara, N., Yoshikawa, K., Imazu, M., Imaoka, T., Takeda, M. (1983) J. Biol. Chem....
A new solvent system benzene-n-hexane-diethylamine (25 : 10 1), was found to show a good separation of cannabidiol, tetrahydrocannabinol and cannabinol in hemp resin by thin-layer chromatography, which Rf-values three constituents were 0.45, 0.35 0.25, respectively. Furthermore, the same successfully applied silica gel column chromatography for isolation resin. Using cocaine hydrochloride as an internal standard gas relative retention times calculated be 1.76, 2.34 2.88, Six kinds hemps...
Abstract A non‐visual pigment melanopsin, which is localized in photosensitive retinal ganglion cells and involved the circadian photoentrainment pupillary responses mammals, phylogenetically close to visual pigments of invertebrates, such as insects cephalopods. Recent studies suggested that melanopsin a bistable drives Gq‐mediated signal transduction cascade, like invertebrate pigments. Because detailed electrophysiological properties are somewhat different between cells, we here expressed...
In the previous study of this series, morphine was shown to be metabolized not only morphine-3-glucuronide, but also morphine-6-glucuronide in rabbits. The present have demonstrated that is common rabbits, guinea pigs, rats, mice and human. As a minor metabolite morphine, normorphine detected urine samples these species by thin-layer chromatography. However, excretion into pigs given conclusive, since similar spot observed on chromatogram extract from untreated animals. Quantitative...
In the previous paper, it was shown that antipyrine oxidized not only to 4-hydroxyantipyrine, but also 3-hydroxymethyl-2-methyl-1-phenyl-3-pyrazolin-5-one which characterized on basis of analytical and spectral data. The present study established latter pathway, oxidation methyl group at 3-position pyrazolinone ring by chemical synthesis authentic sample, demonstrated chromatographically subsequent pathway 2-methyl-1-phenyl-5-oxo-3-pyrazolin-3-carboxylic acid. Possible importance this serial...
Journal Article Metabolism of Drugs: XLI. The Metabolic Fate Strychnine in Rabbit Liver Get access HISAO TSUKAMOTO, TSUKAMOTO From Institute Pharmaceutical Sciences, Faculty Medicine, Kyushu UniversityFukuoka Search for other works by this author on: Oxford Academic PubMed Google Scholar KAZUTA OGURI, OGURI TADASHI WATABE, WATABE HIDETOSHI YOSHIMURA Biochemistry, Volume 55, Issue 4, April 1964, Pages 394–400, https://doi.org/10.1093/oxfordjournals.jbchem.a127900 Published: 01 1964 history...
Animal opsins, light-sensitive G protein-coupled receptors, have been used for optogenetic tools to control protein-dependent signaling pathways. Upon protein activation, the Gα and Gβγ subunits drive different intracellular pathways, leading complex cellular responses. For some purposes, Gα- Gβγ-dependent needs be separately modulated, but these responses are simultaneously evoked due 1:1 stoichiometry of Nevertheless, we show temporal activation using a self-inactivating invertebrate...
Abstract Commutability is where the measurement response for a reference material (RM) same as an individual patient sample with concentration of analyte measured using two or more systems. Assessment commutability essential when RM used in calibration hierarchy to ensure that clinical measurements are comparable across different procedures and at times. The three new Standard Reference Materials ® (SRMs) determining serum total 25-hydroxyvitamin D [25(OH)D], defined sum 2 [25(OH)D ] 3 ],...
Melanopsin functions in intrinsically photosensitive retinal ganglion cells of mammals to regulate circadian clock and pupil constriction. The opsinamide AA92593 has been reported specifically inhibit mouse human melanopsin as a competitive antagonist against retinal; however, the molecular mechanisms underlying its specificity have not resolved. In this study, we attempted identify amino acid residues responsible for susceptibility mammalian melanopsins AA92593. Our cell-based assays...
The first synthesis of three glucuronides narcotics was reported. Codeine glucuronide (Va) prepared by the condensation codeine (Ia) with acetobromo sugar derivative (II) in presence silver carbonate and following removal protecting groups solvolysis hydrolysis sodium methoxide aq. barium hydroxide, respectively. Morphine-6-glucuronide (Vb) synthesized similarly to Va utilizing 3-acetyl-morphine (Ib) as starting material. Morphine-3-glucuronide (VIII) morphine (VI) II hydroxide-acetone. In...