A5048897539- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Heat shock proteins research
- Peptidase Inhibition and Analysis
- Wnt/β-catenin signaling in development and cancer
- Immune Cell Function and Interaction
- Mechanisms of cancer metastasis
- Birth, Development, and Health
- RNA regulation and disease
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Cancer Mechanisms and Therapy
- Cancer-related gene regulation
- Viral gastroenteritis research and epidemiology
- Ubiquitin and proteasome pathways
- T-cell and Retrovirus Studies
- Receptor Mechanisms and Signaling
- T-cell and B-cell Immunology
- Endoplasmic Reticulum Stress and Disease
- Ion Transport and Channel Regulation
National Heart Lung and Blood Institute
2018-2020
National Institutes of Health
2008-2020
University of Maryland, Baltimore
2012-2017
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
2017
University of Baltimore
2013
National Cancer Institute
2006-2008
Center for Cancer Research
2006
The AU-rich elements (AREs) encoded within many mRNA 3' untranslated regions (3'UTRs) are targets for factors that control transcript longevity and translational efficiency. Hsp70, best known as a protein chaperone with well-defined peptide-refolding properties, is to interact ARE-like RNA substrates in vitro. Here, we show cofactor-free preparations of Hsp70 form direct, high-affinity complexes ARE based on specific recognition U-rich sequences by both the ATP- peptide-binding domains....
The RNA-binding protein AUF1 binds AU-rich elements in 3′-untranslated regions to regulate mRNA degradation and/or translation. Many of these mRNAs are predicted microRNA targets as well. An emerging theme post-transcriptional control gene expression is that proteins and microRNAs co-regulate mRNAs. Recent experiments bioinformatic analyses suggest this type co-regulation may be widespread across the transcriptome. Here, we identified from a complex pool cellular examined subset explore...
Hsp70 is a protein chaperone that prevents aggregation and aids folding by binding to hydrophobic peptide domains through reversible mechanism directed an ATPase cycle. However, also binds U-rich RNA including some AU-rich elements (AREs) regulate the decay kinetics of select mRNAs has recently been shown bind stabilize ARE-containing transcripts in cells. Previous studies indicated both ATP- peptide-binding contributed stability Hsp70-RNA complexes ATP might inhibit recruitment. This...
Alternative polyadenylation (APA) produces transcript 3' untranslated regions (3'UTRs) with distinct sequences, lengths, stabilities and functions. We show here that APA products include a class of cryptic nonsense-mediated mRNA decay (NMD) substrates extended 3'UTRs gene- or transcript-level analyses NMD often fail to detect. Transcriptome-wide, the core factor UPF1 preferentially recognizes long 3'UTR APA, leading their systematic downregulation. Counteracting this mechanism,...
Bam32 (B lymphocyte adapter molecule of 32 kDa) is an protein expressed in some hematopoietic cells including B and T lymphocytes. It was previously shown that Bam32-deficient mice have defects various aspects cell activation receptor (BCR)-induced Erk activation, BCR-induced proliferation T-independent antibody responses. In this study, we examined the role using mice. By comparing CD4+ from lymph nodes wild-type mice, found required for optimal TCR-induced cytokine production,...
The serine/threonine kinase Pim-1 directs selected signaling events that promote cell growth and survival is overexpressed in diverse human cancers. expression tightly controlled through multiple mechanisms, including regulation of mRNA turnover. In several cultured models, mitogenic stimulation rapidly induced stabilized PIM1 mRNA, however, vigorous destabilization 4-6 hours later helped restore basal levels. Acceleration turnover coincided with accumulation tristetraprolin (TTP), an...
We previously reported that nitric oxide (NO) reduces the rate of bacteremia and maternal mortality in pregnant rats with uterine infection by Escherichia coli expressing Dr Fimbria (Dr(+) ). The epithelial invasion Dr(+) E. is dependent on expression level its cellular receptor decay accelerating factor (DAF). NO downregulating DAF. In this study, we elucidated role transcription Sp1 RNA binding protein HuR downregulation human DAF NO. generated a series deletion mutant constructs gene...
SUMMARY Alternative polyadenylation (APA) produces transcript 3’ untranslated regions (3’UTRs) with distinct sequences, lengths, stability, and functions. We show here that APA products include a class of cryptic nonsense-mediated mRNA decay (NMD) substrates extended 3’UTRs gene- or transcript-level analyses NMD often fail to detect. Transcriptome-wide, the core factor UPF1 preferentially recognizes long 3’UTR APA, leading their systematic downregulation. Further, we find many events...