The extracellular matrix protein osteopontin (OPN) is a component of histotroph that increases in uterine flushings from pregnant ewes during the peri-implantation period and localized on apical surfaces luminal epithelium (LE) conceptus trophectoderm (Tr). potential involvement OPN implantation adhesion cascade sheep was investigated by examining temporal, spatial, functional relationships between OPN, Muc-1, integrin subunits estrous cycle early pregnancy. Immunoreactive Muc-1 highly...

Keratinocyte growth factor (KGF) is expressed by uterine endometrial epithelial cells during the estrous cycle and pregnancy in pigs, whereas KGF receptor conceptus trophectoderm epithelia. In particular, expression endometrium highest on day 12 of pregnancy. This corresponds to period maternal recognition which signaled large amounts estrogen secreted acting endometrium. Our hypothesis that estrogens origin stimulate expression, and, turn, stimulates proliferation differentiation...

Noninvasive, epitheliochorial placentation in the pig follows a prolonged preimplantation period characterized by migration, spacing and elongation of conceptuses, secretion estrogen for maternal recognition pregnancy. Osteopontin (OPN) is an extracellular matrix protein that binds integrins to promote cell-cell attachment communication. OPN appears play key role conceptus implantation maintenance pregnancy sheep; however, porcine uterus has not been established. Therefore, this study...

Lysophosphatidic acid (LPA), a simple phospholipid-derived mediator with diverse biological actions, acts through the specific G protein-coupled receptors endothelial differentiation gene (EDG) 2, EDG4, EDG7, and GPR23. Recent studies indicate critical role for LPA receptor signaling in embryo implantation. To understand how uterus during pregnancy pigs, we evaluated: 1) spatial temporal expression of uterine endometrium estrous cycle early-stage concepti, 2) levels luminal fluids from d 12...

Experimentation with most human cell types is restricted to the use of lines, and this limits our ability extrapolate interpretations in vivo condition. However, studying trophoblast cells, we have a unique opportunity obtain large quantities readily available tissue. In chapter, outline methodology for purification cells from term placentas. The procedures are based on enzymatic dissociation villous placental tissue, followed by gradient centrifugation immunomagnetic bead purification....

Keratinocyte growth factor/fibroblast factor-7 (KGF/FGF-7) is an established paracrine mediator of hormone-regulated epithelial and differentiation. In all organs studied, KGF uniquely expressed in cells mesenchymal origin. To determine whether its receptor, keratinocyte factor receptor (KGFR) or fibroblast receptor-2IIIb, were the porcine uterus as a potential system mediating progesterone action, we cloned KGFR partial cDNAs from endometrium. expression was detected endometrium by Northern...

Fibroblast growth factor 7 (FGF7) stimulates cell proliferation, differentiation, migration and angiogenesis. The consensus is that FGF7, expressed by mesenchymal cells, binds FGF receptor 2IIIb (FGFR2) on epithelia, thereby mediating epithelial-mesenchymal interactions. pig uterus unique in FGF7 the luminal epithelium (LE) FGFR2 LE, glandular (GE), trophectoderm to effect proliferation differentiated functions during conceptus development implantation. expression uterine LE of pigs...

In the present study quantitative real-time PCR was used to determine expression status of eight imprinted genes (GRB10, H19, IGF2R, XIST, IGF2, NNAT, PEG1 and PEG10) during preimplantation development, in normal fertilized uniparental porcine embryos. The results demonstrated that, all observed embryo samples, a non gene pattern up 16-cell stage development common for most genes. This true classes embryo, regardless parental-origins direction imprint. However, several differentially...

During the implantation period, porcine conceptus secretes interleukin-1beta (IL1B) that may be involved in establishment of pregnancy pigs. However, regulatory mechanism for IL1B receptor expression and function uterine endometrium are not well elucidated. In this study, we determined pigs during pregnancy. subtypes, IL1 type I (IL1R1) accessory protein (IL1RAP) were expressed with being most abundant on Day 12 primarily luminal glandular epithelial cells. Expression IL1R1 mRNA increased...

Chemokines play critical roles in the establishment and maintenance of pregnancy animals. Cysteine-X-cysteine motif chemokine ligand 9 (CXCL9), CXCL10, CXCL11 are involved recruiting immune cells by binding to their shared receptor, CXC receptor 3 (CXCR3), a variety tissues. This study examined expression regulation chemokines CXCL9, CXCL11, CXCR3, role at maternal–conceptus interface pigs. The endometrium expressed CXCR3 stage specifically during pregnancy, with greatest abundance on Day 15...

Female fertility is a highly regulated process involving the synchronized activities of multiple tissues. The underlying genomic regulation tissue synchronization poorly understood. To understand this better we investigated transcriptomes porcine ovary, endometrium, and oviduct at days 0, 3, 6, 9, 12, 15, or 18 oestrous cycle. We analysed transcriptome profiles individual tissues focus on bridging genes shared by two more three tissue-networks were connected forming triangular shape....

The present study was conducted to examine the ability of porcine oocytes achieve male pronuclear (MPN) formation when they are matured and penetrated in vitro under various culture conditions. When cumulus-enclosed were cultured for 24–48 h modified Whitten's medium (pH 7.4) supplemented with 10% follicular fluid, 10 IU eCG/ml, hCG/ml (designated mWM-FG), nuclear maturation reaching metaphase II completed by 36 after start culture. However, there no differences proportions (94–95%)...

Calcium ions have been implicated in the establishment and maintenance of pregnancy, but regulatory mechanisms calcium uterine endometrium conceptus are not well understood pigs. Recently, we showed that TRPV6, a ion channel protein associated with cellular entry ions, is highly expressed during implantation period In present study, investigated spatial temporal expression regulation TRPV6 S100G, an intracellular calcium-regulatory molecule, estrous cycle pregnancy was maintained at...

Successful pregnancy requires an appropriate intrauterine immune response to the conceptus, which is a semiallograft within uterus. We reported that swine leukocyte antigen-DQA (SLA-DQA), major histocompatibility complex (MHC) class II gene, expressed in uterine endometrium at time of conceptus implantation pigs. Because MHC molecules play critical roles system, SLA-DQ was hypothesized be involved regulation during pregnancy. Therefore, we examined expression endometrial tissues obtained...

Prostaglandins (PGs) are important lipid mediators regulating various reproductive processes in many species. In pigs, the expression pattern of PGE2 and PGF2α metabolic enzymes regulatory mechanism controlling levels uterus during pregnancy not completely understood. This study determined endometrial genes (PLA2G4A, PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, CBR1, HPGD) involved metabolism estrous cycle measured uterine tissues flushings at time conceptus implantation pigs. Except studied...

Prostaglandins (PGs) are involved in many reproductive activities including luteolysis, maternal recognition of pregnancy, endometrial gene expression, conceptus development, and parturition domestic animals. However, mechanisms by which PGE2 PGF2alpha modulated the uterine endometrium expression ABCC4 SLCO2A1, responsible for efficient transport PGs across cell membrane, during estrous cycle pregnancy not fully understood pigs. Therefore, we determined genes SLCO2A1 mRNAs were expressed...

To successfully establish and maintain pregnancy in pigs, a variety of factors must work together at the maternal–conceptus interface to form an immune environment appropriate for both mother conceptus. Our transcriptomics study has shown that cluster differentiation ligand 40 (CD40L) its receptor CD40, which are known play important roles regulating cell- antibody-mediated immunity, expressed endometrium during early pregnancy. However, CD40L CD40 signaling system not well understood....

The purinergic system is composed of purine nucleotides, enzymes, transporters, and receptors involved in a variety physiological processes the body, including development, metabolism, immunity, tumorigenesis, reproduction. importance for embryo implantation endometrium pathophysiological conditions has been shown some species. However, expression, regulation, function molecules at maternal-conceptus interface pigs not fully understood. Therefore, we determined expression during estrous...

AMP-activated protein kinase (AMPK) is an important regulator of cellular energy homeostasis, and AMPK contributes to cell growth, apoptosis, autophagy. Although most studies have been performed using two-dimensional (2D) culture, recent demonstrated that the three-dimensional (3D) spheroid technique helpful in various research fields, such as tumor biology, due its resemblance 3D tissue structure. However, role formation has not characterized clearly. This study used knockout line examine...