A5018653700- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Cellular Mechanics and Interactions
- Ubiquitin and proteasome pathways
- Cultural Heritage Management and Preservation
- Nuclear Structure and Function
- Archaeological Research and Protection
- Scottish History and National Identity
Centre de Recherche en Cancérologie de Marseille
2017-2021
Institut Paoli-Calmettes
2021
Institut Pprime
2021
Inserm
2017-2019
Centre National de la Recherche Scientifique
2017-2019
Aix-Marseille Université
2017
Significance Microtubule dynamics is tightly regulated during fundamental biological processes such as mitosis, thereby representing a major target for anticancer therapies. To better understand the molecular mechanisms underlying organization of microtubule network, we systematically investigated proteins interacting with EB1, regulator microtubules dynamics. We identified specific isoform myomegalin, which termed “SMYLE,” that assembles macromolecular complex associated centrosome,...
iASPP is a protein mostly known as an inhibitor of p53 pro-apoptotic activity and predicted regulatory subunit the PP1 phosphatase, which often overexpressed in tumors. We report that associates with microtubule plus-end binding EB1, central regulator dynamics, via SxIP motif. silencing or mutation motif led to defective capture at cortex mitotic cells, leading abnormal positioning spindle. These effects were recapitulated by knockdown membrane-to-cortex linker Myosin-Ic (Myo1c), we...
Abstract The microtubule plus-end binding protein EB1 is the core of a complex network which regulates dynamics during important biological processes such as cell motility and mitosis. We found that iASPP, an inhibitor p53 predicted regulatory subunit PP1 phosphatase, associates with at plus-ends via SxIP motif. iASPP silencing or mutation motif led to defective capture leading edge migrating cells, cortex mitotic cells abnormal positioning spindle. These effects were recapitulated by...