A5078382069- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Birth, Development, and Health
- RNA modifications and cancer
- Growth Hormone and Insulin-like Growth Factors
- Endometriosis Research and Treatment
- Genetic Syndromes and Imprinting
- Cancer Risks and Factors
- Reproductive System and Pregnancy
- Cancer-related molecular mechanisms research
- Histone Deacetylase Inhibitors Research
- Gynecological conditions and treatments
- Pregnancy and preeclampsia studies
- Uterine Myomas and Treatments
- Cancer Cells and Metastasis
- Neonatal Respiratory Health Research
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Congenital Diaphragmatic Hernia Studies
- Pancreatic and Hepatic Oncology Research
- Effects and risks of endocrine disrupting chemicals
- Ovarian cancer diagnosis and treatment
- Synthesis and biological activity
- Pluripotent Stem Cells Research
- Endometrial and Cervical Cancer Treatments
Baylor College of Medicine
2016-2024
University of Pittsburgh
2012-2022
Natera (United States)
2021
UPMC Hillman Cancer Center
2012-2018
Cancer Research Center
2015-2018
Magee-Womens Research Institute
2015-2018
Texas A&M University
2016
Rutgers, The State University of New Jersey
2016
Our previous studies demonstrated that lysine-specific demethylase 1 (LSD1) and histone deacetylases (HDACs) closely interact in controlling growth of breast cancer cells. However, the underlying mechanisms are largely unknown. In this study, we showed knockdown LSD1 expression (LSD1-KD) by RNAi decreased mRNA levels HDAC isozymes triple-negative (TNBC) Small interfering RNA (siRNA)-mediated depletion HDAC5 induced most significant accumulation H3K4me2, a specific substrate LSD1. Combined...
Abstract Purpose: Insulin-like growth factor 1 (IGF1) signaling regulates breast cancer initiation and progression associated phenotypes. We previously identified E-cadherin (CDH1) as a repressor of IGF1 in this study examined how loss affects IGF1R response to anti-IGF1R/insulin receptor (InsR) therapies cancer. Experimental Design: Breast cell lines were used assess altered levels regulate two anti-IGF1R/InsR therapies. In situ proximity ligation assay (PLA) was define interaction between...
Abstract Our early-life environment has a profound influence on developing organs that impacts metabolic function and determines disease susceptibility across the life-course. Using rat model for exposure to an endocrine disrupting chemical (EDC), we show causes dysfunction in adulthood reprograms histone marks liver accelerate acquisition of adult epigenomic signature. This reprogramming persists long after initial exposure, but many reprogrammed genes remain transcriptionally silent with...
Flavin-dependent histone demethylases govern H3K4 methylation and act as important chromatin modulators that are extensively involved in regulation of DNA replication, gene transcription, repair, heterochromatin silencing. While the activities lysine-specific demethylase 1 (LSD1/KDM1A) facilitating breast cancer progression have been well characterized, roles its homolog LSD2 (KDM1B) oncogenesis relatively less understood. In this study, we showed protein level was significantly elevated...
Sex as a biological variable plays critical role both during lung development and in modulating postnatal hyperoxic injury repair. The molecular mechanisms behind these sex-specific differences need to be elucidated. Our objective was determine if the neonatal epigenomic landscape reconfiguration has profound effects on gene expression could underlie sex-biased protection from or susceptibility diseases. Neonatal male female mice (C57BL/6) were exposed hyperoxia (95% FiO, PND 1–5: saccular...
Endosalpingiosis, traditionally regarded as an incidental pathological finding, was recently reported to have association with gynecologic malignancies. To determine the prevalence of endosalpingiosis, we evaluated all benign appearing adnexal lesions using Sectioning and Extensively Examining-Fimbria (SEE-Fim) protocol, queried pathology database for presence malignancy, endometriosis, Walthard nests, paratubal cysts. Using SEE-Fim cysts were 22%, 45%, 33%, 42% respectively, substantially...
Breast cancer is the most commonly diagnosed and second leading cause of death among women in United States. Recently, interest has grown role epigenetics breast development progression. Epigenetic changes such as DNA methylation, histone modifications, abnormal expression non-coding RNAs emerged novel biomarkers diagnosis, therapy, prevention. This review focuses on recent mechanistic findings underlying epigenetic their predictors risk. The rapid progress our understanding opened new...
Abstract The most effective natural prevention against breast cancer is an early first full-term pregnancy. Understanding how the protective effect elicited will inform development of new strategies. To better understand role epigenetics in long-term protection, we investigated parity-induced DNA methylation mammary gland. FVB mice were bred or remained nulliparous and glands harvested immediately after involution (early) 6.5 months following (late), allowing identification both transient...
Background: Tributyltin (TBT) is a persistent and bioaccumulative environmental toxicant. Developmental exposure to TBT has been shown cause fatty liver disease (steatosis), as well increased adiposity in many species, leading its characterization an obesogen. Objective: We aimed determine the long-term effects of developmental on liver. Methods: C57BL/6J mice were exposed dose (0.5mg/kg body weight per day; 3.07μM) below current no observed adverse effect level (NOAEL) via drinking water,...
Scaffold Attachment Factors SAFB1 and SAFB2 are multifunctional proteins that share >70% sequence similarity. knockout (SAFB1−/−) mice display a high degree of lethality, severe growth retardation, infertility in male mice. To assess the vivo role SAFB2, to identify unique functions two paralogs, we generated SAFB2−/− In stark contrast SAFB1−/−, offspring were born at expected Mendelian ratios did not show any obvious defects or fertility. Generation paralog-specific antibodies...
Pancreatic ductal adenocarcinoma (PDAC) has one of the most aggressive cancer histologies, with high recurrence (85%) and a 5-year survival rate 9%. The circulating tumor DNA (ctDNA) is evolving field in setting management for patients advanced solid tumors. In this study, our aim was to investigate potential contributions ctDNA early-stage pancreatic prognostication as early relapse detection disease status monitoring evaluate relationship markers carcinoembryonic antigen (CEA) 19-9 (CA 19-9).
Background Alcohol exposure in utero increases susceptibility to carcinogen‐induced mammary tumorigenesis adult offspring and causes tumors with a more malignant phenotype. This study was conducted identify changes early tumor development that might lead this outcome. Methods Pregnant Sprague–Dawley rats were fed liquid diet containing 6.7% ethanol (alcohol), an isocaloric without alcohol (pair‐fed), or rat chow ad libitum (ad lib) from gestation day 7 until parturition. At birth, female...
Endosalpingiosis (ES) and endometriosis (EM) refer to the growth of tubal endometrial epithelium respectively, outside their site origin. We hypothesize that uterine secretome factors drive ectopic growth. To test this, we developed a mouse model ES EM using tdTomato (tdT) transgenic fluorescent mice as donors. block implantation factors, progesterone knockout (PKO) tdT were created. Fluorescent lesions present after oviduct with without WT endometrium. Implantation was increased (p<0.05)...
Abstract Epigenetic gene silencing plays a critical role in breast tumor aggression. Our previous studies have shown that abnormal activities of histone lysine demethylases (KDMs) are associated with aberrant expression cancer development. However, the precise molecular mechanisms underlying activity KDMs chromatin remodeling and regulation transcription still elusive. Recently, novel mammalian demethylase LSD2 (also known as KDM1B or AOF1) was identified. We possesses to demethylate H3K4...
Abstract Prevention will prove to be the single most effective way of eradicating breast cancer. Currently, natural cancer prevention is an early first full term pregnancy. While it not feasible use pregnancy protect women from cancer, understanding how protective effect elicited inform development new strategies. Women who were pregnant in their twenties are protected thirty forty years later creating a complicated mechanism tease out experimentally. In order understand long-term protection...