- Plant Surface Properties and Treatments
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Tree Root and Stability Studies
- Plant responses to elevated CO2
- Postharvest Quality and Shelf Life Management
- Agricultural Productivity and Crop Improvement
- Soil Management and Crop Yield
- Aluminum toxicity and tolerance in plants and animals
- Microbial bioremediation and biosurfactants
- Polymer-Based Agricultural Enhancements
- Planetary Science and Exploration
- RNA Interference and Gene Delivery
- Pesticide and Herbicide Environmental Studies
- Silicon Effects in Agriculture
- Agriculture, Soil, Plant Science
- Environmental Chemistry and Analysis
- CRISPR and Genetic Engineering
- Clay minerals and soil interactions
- Mycotoxins in Agriculture and Food
- Rice Cultivation and Yield Improvement
- Growth and nutrition in plants
- Plant Virus Research Studies
- Horticultural and Viticultural Research
Wageningen University & Research
2024
University of Göttingen
2021-2024
University of Bonn
2016-2020
RNA interference (RNAi) is a technique used for transgene mediated gene silencing based on the mechanism of post-transcriptional gene-silencing (PTGS). PTGS ubiquitous basic biological phenomenon involved in regulation transcript abundance and plants´ immune response to viruses. also mediates genomic stability by retroelements. RNAi has become an important research tool studying function strong selective suppression target genes. Here we present si-Fi, software design optimization constructs...
Barley (Hordeum vulgare) is more drought tolerant than other cereals, thus making it an excellent model for the study of chemical, transcriptomic and physiological effects water deficit. Roots are first organ to sense soil Therefore, we studied response barley seminal roots different potentials induced by polyethylene glycol (PEG) 8000. We investigated changes in anatomical parameters histochemistry microscopy, quantitative qualitative suberin composition analytical chemistry, transcript...
Waxes are components of the cuticle covering aerial organs plants. Accumulation waxes has previously been associated with protection against water loss, therefore contributing to drought tolerance. However, not much information is known about function individual wax during deficit. We studied role ester synthesis drought. The load on Arabidopsis leaves and stems was increased deficiency. Expression three genes, WSD1, WSD6 WSD7 synthase/diacylglycerol acyltransferase (WS/DGAT or WSD) family...
Water is the most important prerequisite for life and plays a major role during uptake transport of nutrients. Roots are plant organs that take up part water, from surrounding soil. related to root system architecture, growth, age species dependent complex developmental changes in anatomical structures. The latter mainly attributed deposition suberized barriers certain layers cell walls, such as endo- exodermis. With respect water permeability, suberization roots relevant. or hydraulic...
Abstract Wild barley, Hordeum vulgare spp. spontaneum , has a wider genetic diversity than its cultivated progeny, spp . Osmotic stress leads to series of different responses in wild barley seminal roots, ranging from no changes suberization enhanced endodermal certain zones and the formation suberized exodermis, which was not observed modern cultivars studied so far. Further, as response osmotic stress, hydraulic conductivity roots affected but it 2.5‐fold reduced barley. In both...
Summary The precise functions of suberized apoplastic barriers in root water and nutrient transport physiology have not fully been elucidated. While lots research has performed with mutants Arabidopsis , little to no data are available for agricultural crop or tree species. By employing a combined set physiological, histochemical, analytical, physiological methods as well RNA‐sequencing, this study investigated the implications remarkable CRISPR/Cas9‐induced suberization defects young roots...
Summary To acclimate to waterlogged conditions, wetland plants form a barrier radial oxygen loss (ROL) that can enhance transport the root apex. We hypothesized one or more hormones are involved in induction of and searched for such rice. previously identified 98 genes were tissue‐specifically upregulated during ROL formation The R ice XP ro database showed most these highly enhanced by exogenous abscisic acid (ABA). then examined effect ABA on using an biosynthesis inhibitor (fluridone,...
Roots have complex anatomical structures, and certain localized cell layers develop suberized apoplastic barriers. The size tightness of these barriers depend on the growth conditions age root. Such structures result in a composite water solute transport roots.Development along barley seminal roots was detected using various staining methods, suberin amounts apical basal zones were analysed gas chromatography-mass spectometry (GC-MS). hydraulic conductivity ( Lp r ) cortical cells c measured...
Night-time transpiration accounts for a considerable amount of water loss in crop plants. Despite this, there remain many questions concerning night-time - its biological function, regulation and response to stresses such as salinity. The aim the present study was address these on 14- 18-d-old, hydroponically grown barley plants.Plants were either stressed last 4-7 d prior to, during subsequent continuous (24 h), diurnal gravimetric analyses; or subjected salt stress just before then...
Abstract The plant cuticle, which covers all aerial parts of plants in their primary developmental stage, is the major barrier against water loss from leaves. Accumulation cutin and waxes has often been linked to drought tolerance. Here we investigated whether play a role adaption barley mimicked by osmotic stress acting on roots. We compared cuticle properties cultivated ( Hordeum vulgare spp. ) with wild spontaneum ), tested wax composition or amount cuticular transpiration could be future...
Abstract Background Optimum potassium (K) nutrition is essential for high sugar yield and quality of beet crops. Aims We quantified the effect deficiency on dry matter formation during growing season. Methods Sugar beets were grown low, medium, soil K concentrations in a long‐term fertilizer field experiment silt loam alluvial soil. Plants harvested at four time points season, including final harvest. At each harvest, concentration leaves (when applicable) recorded, allowing us to study...
Abstract Aluminum (Al) toxicity is a major factor limiting plant growth in acidic soils. The beneficial element silicon (Si) can mitigate some effects of Al. However, the impact Al on suberized apoplastic barriers roots are largely unknown while Si suberin remains controversial. This study employed physiological, histochemical, and analytical methods, along with Laser Capture Microdissection (LCM) RNA-sequencing, to explore development barley ( Hordeum vulgare L.), species sensitive stress....
Abstract Hardly anything is known regarding the detoxification of surfactants in crop plants, although they are frequently treated with agrochemical formulations. Therefore, we studied transcriptomic changes barley leaves induced response to spraying leaf surfaces two alcohol ethoxylates (AEs). As model surfactants, selected monodisperse tetraethylene glycol monododecyl (C 12 E 4 ) ether and polydisperse BrijL4. Barley plants were harvested 8 h after a 0.1% surfactant solution gene...