A5055056577- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Plant Surface Properties and Treatments
- Coastal wetland ecosystem dynamics
- Plant nutrient uptake and metabolism
- Plant Water Relations and Carbon Dynamics
- Rice Cultivation and Yield Improvement
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Ion-surface interactions and analysis
- Tree Root and Stability Studies
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Pasture and Agricultural Systems
- Forest ecology and management
- Molecular Biology Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Bioenergy crop production and management
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Postharvest Quality and Shelf Life Management
- Bacterial Identification and Susceptibility Testing
- Rangeland and Wildlife Management
- Isotope Analysis in Ecology
Fukui Prefectural University
2015-2025
The University of Tokyo
2008-2011
• To adapt to waterlogging in soil, some gramineous plants, such as maize (Zea mays), form lysigenous aerenchyma the root cortex. Ethylene, which is accumulated during waterlogging, promotes formation. However, molecular mechanism of formation not understood. The aim this study was identify formation-associated genes expressed roots a basis for understanding Maize plants were grown under waterlogged conditions, with or without pretreatment an ethylene perception inhibitor...
Enhancement of oxygen transport from shoot to root tip by the formation aerenchyma and also a barrier radial loss (ROL) in roots is common waterlogging-tolerant plants. Zea nicaraguensis (teosinte), wild relative maize (Zea mays ssp. mays), grows waterlogged soils. We investigated ROL induction Z. nicaraguensis, comparison with (inbred line Mi29), pot soil system hydroponics. Furthermore, depositions suberin exodermis/hypodermis lignin epidermis adventitious grown aerated or stagnant...
Many wetland species form aerenchyma and a barrier to radial O2 loss (ROL) in roots. These features enhance internal diffusion the root apex. Barrier formation rice is induced by growth stagnant solution, but knowledge of dynamics induction early anatomical changes was lacking. ROL short long roots (Oryza sativa L. 'Nipponbare') assessed using cylindrical root-sleeving electrodes methylene blue indicator dye for leakage. Aerenchyma also monitored cross-sections. Microstructure...
Summary Suberin is a complex polymer composed of aliphatic and phenolic compounds. It constituent apoplastic plant interfaces. In many species, including rice ( Oryza sativa ), the hypodermis in outer part roots forms suberized cell wall (the Casparian strip and/or suberin lamellae), which inhibits flow water ions protects against pathogens. To date, there no genetic evidence that an transport barrier hypodermis. We discovered reduced culm number1 rcn1 ) mutant could not develop longer than...
Internal aeration is crucial for root growth in waterlogged soil. A barrier to radial oxygen loss (ROL) can enhance long-distance transport via the aerenchyma tip; a higher concentration at apex enables into anoxic The ROL formed within outer part of roots (OPR). Suberin and/or lignin deposited cell walls are thought contribute barrier, but it unclear which compound main constituent. This study describes gene expression profiles during formation rice determine relative responses suberin...
In flowering plants, the male gametophyte, pollen, develops in anther. Complex patterns of gene expression both gametophytic and sporophytic tissues anther regulate this process. The profiles microspore/pollen tapetum are particular interest. study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed used to characterize separately transcriptomes rice. Expression 11 known specific-genes were consistent previous reports. Based on their spatial temporal...
The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, L2 layer. Until now, it has not been possible to delineate their transcriptomes due technical difficulties in separating two types. In present study, we characterized separated of rice microspore/pollen using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns 28,141 anther-expressed genes were classified into 20 clusters,...
Internal aeration is crucial for root growth under waterlogged conditions. Many wetland plants have a structural barrier that impedes oxygen leakage from the basal part of roots called radial loss (ROL) barrier. ROL barriers reduce transported via aerenchyma to tips, enabling long-distance transport cell respiration at tip. Because tip does not an barrier, some transferred released into soil, where it oxidizes and detoxifies toxic substances (e.g., sulfate Fe2+) around are located outer...
Co-expression networks systematically constructed from large-scale transcriptome data reflect the interactions and functions of genes with similar expression patterns are a powerful tool for comprehensive understanding biological events mining novel genes. In Arabidopsis (a model dicot plant), high-resolution co-expression have been very large microarray datasets these publicly available as online information resources. However, rice monocot plant) limited so far, making it difficult...
Internal aeration is crucial for root growth under waterlogged conditions. Some wetland plants have a structural barrier that impedes oxygen leakage from the basal part of roots called radial loss (ROL) barrier. The ROL reduces transported via aerenchyma to tips, enabling into anoxic soil. some develop an conditions, while they remain leaky well-drained or aerated main components inducible are thought be suberin and lignin deposited at outer cellular space (apoplast) in roots. On other hand,...
Abstract Background and Aims Internal root aeration is essential for growth in waterlogged conditions. Aerenchyma provides a path oxygen to diffuse the roots. In most wetland species, including rice, barrier radial loss (ROL) allows more of tip, enabling into anoxic soil. Most dryland crops, barley, do not form ROL barrier. We previously found that abscisic acid (ABA) signalling involved induction formation rice during waterlogging. Although typically does tight roots aerated conditions, an...
In rice (Oryza sativa) roots, lysigenous aerenchyma, which is created by programmed cell death and lysis of cortical cells, constitutively formed under aerobic conditions, its formation further induced oxygen-deficient conditions. Ethylene involved in the induction aerenchyma formation. reduced culm number1 (rcn1) a mutant gene encoding ATP-binding cassette transporter RCN1/OsABCG5 defective. Here, we report that was roots rcn1 grown stagnant deoxygenated nutrient solution (i.e. mimic...
Plant hormones can act in synergistic and antagonistic ways response to biotic abiotic stresses plant growth development. Thus, a technique is needed simultaneously determine the distributions concentrations of several hormones. Previously, we reported that localizations two [cytokinin (CK) abscisic acid (ABA)] be visualized tissue using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). In MALDI-MS, however, self-ionization an organic matrix occasionally interferes...
Summary To acclimate to waterlogged conditions, wetland plants form a barrier radial oxygen loss (ROL) that can enhance transport the root apex. We hypothesized one or more hormones are involved in induction of and searched for such rice. previously identified 98 genes were tissue‐specifically upregulated during ROL formation The R ice XP ro database showed most these highly enhanced by exogenous abscisic acid (ABA). then examined effect ABA on using an biosynthesis inhibitor (fluridone,...
A barrier to radial oxygen loss (ROL), which reduces the of transported via aerenchyma root tips, enables roots wetland plants grow into anoxic/hypoxic waterlogged soil. However, little is known about its genetic regulation. Quantitative trait loci (QTLs) mapping can help understand factors that regulate formation. Rice (Oryza sativa) inducibly forms an ROL under stagnant conditions, while a few constitutively form one aerated conditions. Here, we evaluated formation constitutive in total...
Abstract To acclimate to hypoxic waterlogged conditions, the roots of wetland plants form a radial oxygen loss (ROL) barrier that can promote diffusion root tips. We hypothesized low-nitrate concentrations occur after molecular is consumed in soils are an environmental trigger for ROL formation rice (Oryza sativa). previously identified 128 tissue-specific up/downregulated genes during formation. The RiceXPro database showed many these were differentially regulated response nitrogen...
Plant hormones act as important signaling molecules that regulate responses to abiotic stress well plant growth and development. Because their concentrations of control the physiological in target tissue, it is know distributions tissues. However, difficult determine hormone concentration on tissue a result limitations conventional methods. Here, we report first multi-imaging two hormones, one cytokinin [i.e., trans-zeatin (tZ)] abscisic acid (ABA) using new technology, matrix-assisted laser...
Root growth into hypoxic or anoxic waterlogged soil relies on internal aeration in plants. The plant hormone ethylene helps adapt to waterlogging by inducing the formation of aerenchyma, which provides a low-resistance pathway for transport oxygen from shoot root apex. Waterlogging-susceptible crops including barley start form aerenchyma after suffering stress. But can be fatal if is not fast enough. Here, we investigated whether pre-treating with ethephon, an ethylene-releasing...
Many crops are sensitive to waterlogging. A small, fast-growing grass, Brachypodium distachyon (Bd21), whose genome has been sequenced, is a new model for studying cereal such as wheat and barley, developing novel biomass grasses. However, its waterlogging tolerance oxygen transport properties not known. Here, we show that in stagnant deoxygenated nutrient solution, which mimics waterlogged soil, B. grows poorly does increase the number of newly formed roots. In both aerated conditions,...
Passage cells are frequently found in the exodermis and endodermis of roots. Because passage lack an apoplastic diffusion barrier, they thought to provide pathways for transport nutrients entrance endomycorrhizal fungi. Exodermal possess Casparian strips but not suberin lamellae. So far, exodermal have been associated with a particular internal structure. In some wetland plants, outer part root (i.e., epidermis, exodermis, sclerenchyma) emerging lateral primordia has oxygen leaky zone called...
Abstract Background Detailed datasets containing root system and its architecture in soil are required to improve understanding of resource capture by roots. However, most the study methods have paid little attention make preserve whole specimens. This introduces sampling equipment that makes entire specimen with minimum impairment without displacement spatial arrangement boxes. The objectives assess: whether can rapidly sample system; surface area is measurable from a scanned digital image...
Submergence during germination impedes aerobic metabolisms and limits the growth of most higher plants. However, some wetland plants including rice can germinate under submerged conditions. It has long been hypothesized that first elongating shoot tissue, coleoptile, acts as a snorkel to acquire atmospheric oxygen (O2) initiate leaf elongation seminal root emergence. Here, we obtained direct evidence for this hypothesis by visualizing spatiotemporal O2 dynamics in using planar optode system....