A5090866306- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- DNA and Nucleic Acid Chemistry
- DNA and Biological Computing
- Epigenetics and DNA Methylation
- Plant Genetic and Mutation Studies
- Reproductive Biology and Fertility
- Cancer-related Molecular Pathways
- Mitochondrial Function and Pathology
Centre National de la Recherche Scientifique
2019-2025
Inserm
2025
Institut de Biologie de l'École Normale Supérieure
2023-2025
Université Paris Sciences et Lettres
2025
Institut de Biologie Intégrative de la Cellule
2019-2024
École Normale Supérieure - PSL
2023-2024
CEA Paris-Saclay
2019-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2019-2021
Université Claude Bernard Lyon 1
2021
Université Paris-Saclay
2020-2021
Abstract Large vertebrate genomes duplicate by activating tens of thousands DNA replication origins, irregularly spaced along the genome. The spatial and temporal regulation process is not yet fully understood. To investigate dynamics, we developed a methodology called RepliCorr, which uses correlation between patterns observed on stretched single-molecule obtained either combing or high-throughput optical mapping. analysis revealed two independent spatiotemporal processes that regulate...
Abstract The activation of eukaryotic DNA replication origins needs to be strictly controlled at multiple steps in order faithfully duplicate the genome and maintain its stability. How checkpoint recovery adaptation protein Polo-like kinase 1 (Plk1) regulates firing during non-challenged S phase remained an open question. Using fiber analysis, we show that immunodepletion Plk1 Xenopus vitro system decreases fork density initiation frequency. Numerical analyses suggest reduces overall...
Polo-like kinase 1 (Plk1) is a cell cycle essential for mitosis progression, but also important checkpoint recovery and adaptation in response to DNA damage replication stress. However, although Plk1 expressed S phase, little known about its function during unperturbed replication. Using Xenopus laevis egg extracts, mimicking early embryonic replication, we demonstrate that simultaneously recruited chromatin with pre-replication proteins where it accumulates throughout phase. Further, found...
Abstract Metazoan genomes are duplicated by the coordinated activation of clusters replication origins at different times during S phase, but underlying mechanisms this temporal program remain unclear early development. Rif1, a key timing factor, inhibits origin firing recruiting protein phosphatase 1 (PP1) to chromatin counteracting phase kinases. We have previously described that Rif1 depletion accelerates Xenopus laevis embryonic cell cycles. Here, we find in absence patterns foci change...
During the first rapid divisions of early development in many species, DNA:cytoplasm ratio increases until midblastula transition (MBT) when transcription resumes and cell cycles lengthen. S phase is very embryos, about 20–30 times faster than differentiated cells. Using a combination DNA fiber studies Xenopus laevis embryonic vitro replication system, we show that slows down shortly after MBT owing to genome wide decrease eye density. Increasing dNTP pool did not accelerate or increase...
During cell division, the duplication of genome starts at multiple positions called replication origins. Origin firing requires interaction rate-limiting factors with potential origins during S(ynthesis)-phase cycle. Origins fire as synchronous clusters which is proposed to be regulated by intra-S checkpoint. By modelling unchallenged, checkpoint-inhibited and checkpoint protein Chk1 over-expressed pattern single DNA molecules from Xenopus sperm chromatin replicated in egg extracts, we...
Abstract Large vertebrate genomes duplicate by activating tens of thousands DNA replication origins, irregularly spaced along the genome. The spatial and temporal regulation process is not yet fully understood. To investigate dynamics, we developed a methodology called RepliCorr, which uses correlation between patterns observed on stretched single-molecule obtained either combing or high-throughput optical mapping. analysis revealed two independent spatiotemporal processes that regulate...
<title>Abstract</title> <bold>Background</bold>: During cell division, the duplication of genome starts at multiple positions called replication origins. Origin firing requires interaction rate-limiting factors with potential origins during S(ynthesis)-phase cycle. Origins fire as synchronous clusters which is proposed to be regulated by intra-S checkpoint. <bold>Results</bold>: By modelling unchallenged, checkpoint-inhibited and checkpoint protein Chk1 over-expressed pattern single DNA...
Abstract During cell division, the duplication of genome starts at multiple positions called replication origins. Origin firing requires interaction rate-limiting factors with potential origins during S(ynthesis)-phase cycle. Origins fire as synchronous clusters which is proposed to be regulated by intra-S checkpoint. By modelling unchallenged, checkpoint-inhibited and checkpoint protein Chk1 over-expressed pattern single DNA molecules from Xenopus sperm chromatin replicated in egg extracts,...