A5021926744- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Caveolin-1 and cellular processes
- Cellular Mechanics and Interactions
- DNA and Nucleic Acid Chemistry
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Hippo pathway signaling and YAP/TAZ
- Reproductive Biology and Fertility
- Tendon Structure and Treatment
- Plant Genetic and Mutation Studies
- Force Microscopy Techniques and Applications
Centre National de la Recherche Scientifique
2015-2023
CEA Paris-Saclay
2019-2023
Institut de Biologie Intégrative de la Cellule
2015-2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2023
Université Paris-Saclay
2020-2023
Laboratoire de Physique de l'ENS de Lyon
2021
ABSTRACT Focal adhesions are composed of transmembrane integrins, linking the extracellular matrix to actomyosin cytoskeleton, via cytoplasmic proteins. Adhesion depends on activation integrins. Talin and kindlin proteins intracellular activators integrins that bind β-integrin tails. Integrin clustering through ligands guide organization adhesion complexes. However, roles talin in this process poorly understood. To determine contribution talin, kindlin, lipids integrin clustering, we used a...
Abstract The activation of eukaryotic DNA replication origins needs to be strictly controlled at multiple steps in order faithfully duplicate the genome and maintain its stability. How checkpoint recovery adaptation protein Polo-like kinase 1 (Plk1) regulates firing during non-challenged S phase remained an open question. Using fiber analysis, we show that immunodepletion Plk1 Xenopus vitro system decreases fork density initiation frequency. Numerical analyses suggest reduces overall...
DNA replication in higher eukaryotes initiates at thousands of origins according to a spatio-temporal program. The ATR/Chk1 dependent checkpoint inhibits the activation later firing origins. In Xenopus vitro system initiations are not sequence and 2-5 grouped clusters that fire different times despite very short S phase. We have shown temporal program is stochastic level single clusters. It unclear how late but permits origin early Here, we analyze role Chk1 sperm nuclei replicating egg...
In multicellular eukaryotic organisms, the initiation of DNA replication occurs asynchronously throughout S-phase according to a regulated timing program. Here, using Xenopus egg extracts, we showed that Yap (Yes-associated protein 1), downstream effector Hippo signalling pathway, is required for control dynamics. We found recruited chromatin at start and identified Rif1, major regulator program, as novel binding protein. Furthermore, show either or Rif1 depletion accelerates dynamics by...
Polo-like kinase 1 (Plk1) is a cell cycle essential for mitosis progression, but also important checkpoint recovery and adaptation in response to DNA damage replication stress. However, although Plk1 expressed S phase, little known about its function during unperturbed replication. Using Xenopus laevis egg extracts, mimicking early embryonic replication, we demonstrate that simultaneously recruited chromatin with pre-replication proteins where it accumulates throughout phase. Further, found...
During the first rapid divisions of early development in many species, DNA:cytoplasm ratio increases until midblastula transition (MBT) when transcription resumes and cell cycles lengthen. S phase is very embryos, about 20–30 times faster than differentiated cells. Using a combination DNA fiber studies Xenopus laevis embryonic vitro replication system, we show that slows down shortly after MBT owing to genome wide decrease eye density. Increasing dNTP pool did not accelerate or increase...
During cell division, the duplication of genome starts at multiple positions called replication origins. Origin firing requires interaction rate-limiting factors with potential origins during S(ynthesis)-phase cycle. Origins fire as synchronous clusters which is proposed to be regulated by intra-S checkpoint. By modelling unchallenged, checkpoint-inhibited and checkpoint protein Chk1 over-expressed pattern single DNA molecules from Xenopus sperm chromatin replicated in egg extracts, we...
Abstract The cohesion of tissues requires that cells establish cell-cell junctions. Cells contact each other by forming Arp2/3-dependent lamellipodia before they initiate the formation cadherin-based adherens junctions (AJs). Maturing AJs then assemble actin under force though a mechanosensitive complex comprising actin-binding proteins α-catenin, vinculin and VASP, which individually act on nucleation, elongation organisation filaments in different ways. However, activity ternary these...
Abstract In multicellular eukaryotic organisms, the initiation of DNA replication occurs asynchronously throughout S-phase according to a regulated timing program. Here, using Xenopus egg extracts, we showed that Yap (Yes-associated protein 1), downstream effector Hippo signaling pathway, is required for control dynamics. We found recruited chromatin at start and depletion accelerates dynamics by increasing number activated origins. Furthermore, identified Rif1, major regulator program, as...
<title>Abstract</title> <bold>Background</bold>: During cell division, the duplication of genome starts at multiple positions called replication origins. Origin firing requires interaction rate-limiting factors with potential origins during S(ynthesis)-phase cycle. Origins fire as synchronous clusters which is proposed to be regulated by intra-S checkpoint. <bold>Results</bold>: By modelling unchallenged, checkpoint-inhibited and checkpoint protein Chk1 over-expressed pattern single DNA...
Abstract During cell division, the duplication of genome starts at multiple positions called replication origins. Origin firing requires interaction rate-limiting factors with potential origins during S(ynthesis)-phase cycle. Origins fire as synchronous clusters which is proposed to be regulated by intra-S checkpoint. By modelling unchallenged, checkpoint-inhibited and checkpoint protein Chk1 over-expressed pattern single DNA molecules from Xenopus sperm chromatin replicated in egg extracts,...
Abstract Focal adhesions are important mechanosensitive structures, composed of transmembrane integrins, linking the extracellular matrix to actomyosin cytoskeleton, via cytoplasmic proteins. Cellular adhesion depends on activation integrins by intracellular mechanisms. Talin and kindlin major activators that recruited inner membrane bind β-integrin tails. Many studies showed importance integrin clustering how organization ligands guides nanoscale complexes. However, roles talin in this...