Plant-parasitic nematodes (PPNs) are highly damaging pests responsible for heavy losses in worldwide productivity a significant number of important plant crops. Common pest management strategies rely on the use synthetic chemical nematicides, which have led to serious concerns regarding their impacts human health and environment. Plant natural products, or phytochemicals, can provide good source agents sustainable control PPNs, due intrinsic characteristics such as higher biodegradability,...

Soil-dwelling plant parasitic nematodes (PPNs) are important potato pests that cause lesions and/or change roots structure, leading to reduced crop fitness and productivity. Research on the cellular subcellular mechanisms of PPNs infection development can resort field plants or seedlings under greenhouse conditions. Field studies more representative natural environments but subjected unpredictability environmental conditions heavily influence research outcomes. Greenhouse allow higher...

Abstract Antimicrobial resistance among Staphylococcus species, including multidrug-resistant and biofilm-forming strains, poses a critical threat to global health, demanding innovative therapeutic solutions. In this context, study explores the antimicrobial antibiofilm potential of aquatic plant Hydrocleys nymphoides as promising alternative. Extracts from plant's leaves roots were obtained using solvents increasing polarity tested against five key pathogenic species: S. aureus,...

Pine wilt disease (PWD) is a complex integrating three major agents: the pathogenic agent, pinewood nematode Bursaphelenchus xylophilus; insect-vector Monochamus spp.; and host pine tree, Pinus sp. Since early 80's, notion that another namely bacteria, may play role in PWD has been gaining traction, however of bacteria still unknown. The present work supports possibility some B. xylophilus-associated significant development this disease. This inferred as consequence of: (i) phenotypic...

Abstract VEGF and TGF‐β1 are cytokines that stimulate tissue invasion angiogenesis. These factors considered as molecular targets for the therapy of glioblastoma. Bevacizumab, a recombinant humanized monoclonal antibody developed against VEGF, inhibits endothelial cell proliferation vessel formation. Flavonoids obtained from Dimorphandra mollis Croton betulaster have been described inhibitors human glioblastoma derived line. levels were dosed by ELISA in GL‐15 line treated with bevacizumab...

Pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease, a serious threat to global forest populations conifers, especially Pinus spp. A time-course study essential oils (EOs) 2-year-old halepensis, pinaster, pinea and sylvestris following inoculation with PWN was performed. The constitutive induced EOs components were analyzed at both wounding or areas whole plant level. enantiomeric ratio optically active main also evaluated. External symptoms...

During cell division, the guanine nucleotide exchange factor (GEF) ECT2 activates RhoA in a narrow zone at equator anaphase. consists of three BRCT domains (BRCT0, 1, and 2), catalytic GEF, pleckstrin homology (PH) domain. How conserved spatially temporally control activity remains unclear. We reveal that each domain makes distinct contributions to function. find BRCT0 contributes to, BRCT1 is essential for, activation BRCT2 integrates two functions: GEF inhibition RACGAP1 binding, which...

Control of the pinewood nematode (PWN), causal agent pine wilt disease, can be achieved through trunk injection nematicides; however, many pesticides have been linked to environmental and human health concerns. Essential oils (EOs) are suitable alternatives due their biodegradability low toxicity mammals. These complex mixtures plant volatiles often display multiple biological activities synergistic interactions between compounds. The present work profiled eight EOs against PWN in comparison...

Traditional pesticides are based on toxic compounds that can reduce biodiversity, degrade the environment, and contribute to less healthy living. Plant allelochemicals provide more environmentally friendly sustainable alternatives. Essential oils (EOs) complex mixtures of plant secondary metabolites show strong biological activities. In present study, EOs Cymbopogon citratus were screened for activity against pinewood nematode (PWN), causal agent pine wilt disease. To understand their...

Acetone was investigated and found to be an appropriate alternative Triton X-100 as a solvent of essential oils in bioassays aimed investigate their effects on pinewood nematode (Bursaphelenchus xylophilus) mortality. Therefore it used dilution agent screen the effectiveness fifty two against this pest. Thirteen were highly effective, resulting more than 90% mortality at 2 mg/mL, with six them 100% LC₁₀₀ values ranged between 0.50 mg/mL 0.83 for Origanum vulgare Satureja montana,...

<p style="text-align: justify;"><strong>Aim</strong>: To test the hypothesis that shading of fruiting zone plants might reduce yield losses caused by excessive exposure to sun while avoiding most damaging effects associated with reduced radiation.</p><p justify;"><strong>Methods and results</strong>: A number grapevine rows were shaded a double layered white plastic netting on their south-facing side, from ground about 20 cm above cluster zone. Data...

The aim of this study is to understand the biological role Serratia quinivorans BXF1, a bacterium commonly found associated with Bursaphelenchus xylophilus, plant parasitic nematode responsible for pine wilt disease. Therefore, we studied strain BXF1 effect in We that promoted vitro reproduction. Moreover, presence bacteria led absence chitinase gene (Bxcht-1) expression, suggesting an bacterial Nevertheless, was unable colonize interior, bind its cuticle high affinity or protect from...

DNA replication in higher eukaryotes initiates at thousands of origins according to a spatio-temporal program. The ATR/Chk1 dependent checkpoint inhibits the activation later firing origins. In Xenopus vitro system initiations are not sequence and 2-5 grouped clusters that fire different times despite very short S phase. We have shown temporal program is stochastic level single clusters. It unclear how late but permits origin early Here, we analyze role Chk1 sperm nuclei replicating egg...

When a species is introduced in new location, it common for to establish itself when finds favorable conditions the receptor community with regard interspecific interactions native species. The azooxanthellate corals Tubastraea coccinea and tagusensis are invasive Caribbean Sea, Gulf of Mexico, Brazilian Southwest Atlantic. They successful competitors space, have multiple reproductive modes, high larval dispersion recruitment, but studies on food trophic relationships genus still scarce. In...

Exposure to either neutral red, nile blue A or methylene extended the developmental period of A. aegypti (L.) larvae. The rate pupation decreased and mortality increased as a function increasing concentration. Pupal weights were adversely affected in females but not males. These adverse effects caused by commonly used “vital” dyes demonstrate need for caution their use.