A5066952724- Salivary Gland Disorders and Functions
- Sepsis Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- COVID-19 Clinical Research Studies
- Nosocomial Infections in ICU
- Nerve injury and regeneration
- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 detection and testing
- S100 Proteins and Annexins
- Biosensors and Analytical Detection
- Mycobacterium research and diagnosis
- Advanced Biosensing Techniques and Applications
- Wound Healing and Treatments
- Cancer-related molecular mechanisms research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
George Washington University
2024-2025
Washington University Medical Center
2024
National Institute of Dental and Craniofacial Research
2022-2023
National Institutes of Health
2022-2023
Abstract The mechanisms that prevent regeneration of irradiated (IR) salivary glands remain elusive. Bulk RNAseq IR versus non-IR human showed neurotrophin signaling is highly disrupted post-radiation. Neurotrophin receptors (NTRs) were significantly upregulated in myoepithelial cells (MECs) post-IR, and single cell revealed MECs pericytes, duct are the main sources ligands. Using two ex vivo models, we show nerve growth factor (NGF) induces expression MEC genes during development,...
Understanding the transcriptional landscape that results in chronic salivary hypofunction after irradiation will help identify injury mechanisms and develop regenerative therapies. We present scRNA-seq analysis from control irradiated murine parotid glands collected 10 months irradiation. a population of secretory cells defined by specific expression Etv1, which may be an acinar cell precursor. Acinar Etv1+ express Ntrk2 Erbb3, respectively while ligands for these receptors are expressed...
Secondary opportunistic coinfections are a significant contributor to morbidity and mortality in intensive care unit (ICU) patients, but can be difficult identify. Presently, new blood RNA biomarkers were tested ICU patients diagnose viral, bacterial, biofilm coinfections.
SUMMARY Translational frameworks to understand the chronic loss of salivary dysfunction that follows after clinical irradiation, and development regenerative therapies remain an unmet need. Understanding transcriptional landscape long irradiation treatment results in hypofunction will help identify injury mechanisms develop address this Advances single cell (sc)RNAseq have made it possible previously uncharacterized types within tissues uncover gene regulatory networks mediate cell-cell...