A5070270181- Salivary Gland Disorders and Functions
- Effects of Radiation Exposure
- Head and Neck Cancer Studies
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Oral health in cancer treatment
- melanin and skin pigmentation
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Sirtuins and Resveratrol in Medicine
- NF-κB Signaling Pathways
- Metabolism, Diabetes, and Cancer
- Genomics, phytochemicals, and oxidative stress
- Adenosine and Purinergic Signaling
- Heavy Metal Exposure and Toxicity
- Cell Image Analysis Techniques
- AI in cancer detection
- Trace Elements in Health
- Axon Guidance and Neuronal Signaling
- Growth Hormone and Insulin-like Growth Factors
- Metabolomics and Mass Spectrometry Studies
- Fibroblast Growth Factor Research
University of Arizona
2015-2024
University of Arizona Cancer Center
2021-2023
Thomas Jefferson University
2010
Cancer Genetics (United States)
2010
University of Colorado Denver
2009
University of Colorado Health
2003-2006
University of Colorado Anschutz Medical Campus
2003
Protein kinase C (PKC) delta is an essential regulator of mitochondrial dependent apoptosis in epithelial cells. We have used the PKCdelta(-/-) mouse to ask if loss PKCdelta protects salivary glands against gamma-irradiation-induced vivo and explore mechanism underlying protection from apoptosis. show that gamma-irradiation results a robust induction parotid wild type mice, whereas suppressed by greater than 60% mice. Primary cells mice are defective as indicated suppression...
Summary Aging of the world population and a concomitant increase in age‐related diseases disabilities mandates search for strategies to healthspan, length time an individual lives healthy productively. Due decline immune system, infectious remain among top 5–10 causes mortality morbidity elderly, improving function during aging remains important aspect healthspan extension. Calorie restriction ( CR ) more recently rapamycin (rapa) feeding have both been used extend lifespan mice. Preciously...
Radiation is a primary or secondary therapeutic modality for treatment of head and neck cancer. A common side effect irradiation to the region xerostomia caused by salivary gland dysfunction. Approximately 40,000 new cases result from radiation in United States each year. The ensuing hypofunction results significant morbidity diminishes effectiveness anti-cancer therapies as well quality life these patients. Previous studies rat model have shown no correlation between induction apoptosis...
Abstract Salivary glands are indirectly damaged during radiotherapy for head and neck cancer, resulting in acute chronic hyposalivation. Current treatments radiation-induced hyposalivation do not permanently restore function to the gland; therefore, more mechanistic understanding of damage response is needed identify therapeutic targets lasting restoration. Energy metabolism reprogramming has been observed cancer wound healing models provide necessary fuel cell proliferation; however, there...
Abstract Background Radiotherapy for head and neck cancer results in severe chronic salivary gland dysfunction most individuals. This significant side effects including xerostomia, dysphagia, malnutrition which are linked to reductions patients' quality of life. Currently there few xerostomia treatment approaches that provide long-term without effects. To address this problem we investigated the potential post-therapeutic IGF-1 reverse radiation-induced dysfunction. Methods FVB mice were...
Chronic damage to the salivary glands is a common side effect following head and neck irradiation. It hypothesized that irreversible occurs immediately after radiation; however, previous studies with rat models have not shown causal role for apoptosis in radiation-induced injury. We report etoposide gamma irradiation induce of acinar cells from FVB control mice vitro vivo; reduced transgenic expressing constitutively activated mutant Akt1 (myr-Akt1). Expression myr-Akt1 results significant...
The current standard of care for head and neck cancer includes surgical resection the tumor followed by targeted radiation. This radiotherapy results in a multitude negative side effects adjacent normal tissues. Autophagy is cellular mechanism that could be to ameliorate these based on its role homeostasis. In this study, we utilized Atg5f/f;Aqp5-Cre mice which harbor conditional knockout Atg5, salivary acinar cells. These autophagy-deficient display increased radiosensitivity. Treatment...
Radiotherapy for head and neck tumors often results in persistent loss of function salivary glands. Patients suffering from impaired frequently terminate treatment prematurely because reduced quality life caused by malnutrition other debilitating side-effects. It has been previously shown mice expressing a constitutively active form Akt (myr-Akt1), or pretreated with IGF1, apoptosis is suppressed, which correlates maintained gland measured stimulated flow. Induction cell cycle arrest may be...
Tumor cells grow in nutrient- and oxygen-deprived microenvironments adapt to the suboptimal growth conditions by altering their metabolic pathways. This adaptation process commonly results a tumor phenotype that displays high rate of aerobic glycolysis aggressive characteristics. The glucose regulatory molecule, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), is bifunctional enzyme central glycolytic flux downstream stress sensor AMP-activated protein kinase (AMPK), which has...
Radiotherapy is the primary treatment for patients with head and neck cancer, which account roughly 500,000 annual cases worldwide. Dysfunction of salivary glands associated conditions like xerostomia dysphagia are often developed by these patients, greatly diminishing their life quality. Current preventative palliative care fail to deliver an improvement in quality life, thus accentuating need regenerative therapies. In this study, a model label retaining cells (LRCs) murine was developed,...
Background Treatment of head and neck cancer with radiation often results in damage to surrounding normal tissues such as salivary glands. Permanent loss function the glands leads patients discontinue treatment due incapacitating side effects. It has previously been shown that IGF-1 suppresses radiation-induced apoptosis enhances G2/M arrest leading preservation gland function. In an effort recapitulate effects IGF-1, well increase likelihood translating these findings clinic, small molecule...
Autophagy is a catabolic pathway utilized to maintain balance among the synthesis, degradation, and recycling of cellular components, thereby playing role in cell growth, development, homeostasis. Previous studies revealed that conditional knockout essential member(s) autophagy variety tissues causes changes structure function these tissues. Acinar cell-specific expression knocked-in Cre recombinase through control aquaporin 5 (Aqp5) promoter/enhancer ( Aqp5-Cre) allows us specifically...
Defects in basal autophagy limit the nutrient supply from recycling of intracellular constituents. Despite our understanding prosurvival role macroautophagy/autophagy, how deprivation, caused by compromised autophagy, affects oncogenic KRAS-driven tumor progression is poorly understood. Here, we demonstrate that conditional impairment gene Atg5 (atg5-KO) extends survival KRAS