- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Molecular Biology Research
- Horticultural and Viticultural Research
- Plant Physiology and Cultivation Studies
- Lipid metabolism and biosynthesis
- Berry genetics and cultivation research
- Amino Acid Enzymes and Metabolism
- Plant tissue culture and regeneration
- Enzyme Catalysis and Immobilization
- Postharvest Quality and Shelf Life Management
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Nitrogen and Sulfur Effects on Brassica
- Plant responses to elevated CO2
- Plant and Fungal Interactions Research
- Plant Pathogens and Fungal Diseases
James Hutton Institute
2023
East Malling Research (United Kingdom)
2022
Genomics (United Kingdom)
2022
National Institute of Agricultural Botany
2021-2022
University of Essex
2015-2019
University of the Free State
2013
Summary The circadian system allows plants to coordinate metabolic and physiological functions with predictable environmental variables such as dusk dawn. This endogenous oscillator is comprised of biochemical transcriptional rhythms that are synchronized a plant's surroundings via signals, including light temperature. We have used chlorophyll fluorescence techniques describe PSII operating efficiency ( ) in the chloroplasts Arabidopsis thaliana . These oscillations appear be influenced by...
Abstract The sensitivity of the circadian system to light allows entrainment clock, permitting coordination plant metabolic function and flowering time across seasons. Light affects via both photoreceptors, such as phytochromes cryptochromes, sugar production by photosynthesis. In present study, we introduce a constitutively active version phytochrome B-Y276H (YHB) into wild-type null backgrounds Arabidopsis (Arabidopsis thaliana) distinguish effects photoreceptor signaling on clock from...
The circadian system optimizes cellular responses to stress, but the signaling pathways that convey metabolic consequences of stress into this molecular timekeeping mechanism remain unclear. Redox regulation SAL1 phosphatase during abiotic initiates a pathway from chloroplast nucleus by regulating accumulation metabolite, 3′-phosphoadenosine 5′-phosphate (PAP). Consequently, PAP accumulates in response redox and inhibits activity exoribonucleases (XRNs) cytosol. We demonstrated osmotic...
The objective of our study was the alignment microsatellite or simple sequence repeat (SSR) marker data across germplasm collections cherry within Europe. Through European Cooperative program for Plant Genetic Resources ECPGR, a number had previously been analysed using standard sets SSR loci. However, until now these datasets remained unaligned. We used combination reference genotypes and ad-hoc selections to compile central dataset representing as many alleles possible from national...
Abstract Simple sequence repeat (SSR) microsatellite markers have been extensively used to identify duplication and analyse genetic diversity in germplasm collections of apple. Here, we present findings from the use a standard set SSR loci managed repropagation significant international collection: UK National Fruit Collection (NFC). A subset eight was deemed sufficient distinguish all apart clonal relatives across sample 1995 accessions, with single exception being one pair full siblings....
The circadian system induces rhythmic variation in a suite of biochemical and physiological processes that serve to optimise plant growth diel cycles. To be greatest utility, these behaviors are coordinated with regular environmental changes such as the rising setting sun. Photoreceptors, along metabolites produced during photosynthesis, act synchronise internal timing mechanism lighting cues. We have recently shown phototropins help maintain robust rhythms photosynthetic operating...
The circadian clock system acts as an endogenous timing reference that coordinates many metabolic and physiological processes in plants. Previous studies have shown the application of osmotic stress delays rhythms via 3'-phospho-adenosine 5'-phosphate (PAP), a retrograde signalling metabolite is produced response to redox within organelles. PAP accumulation leads inhibition exoribonucleases (XRNs), which are responsible for RNA degradation. Interestingly, we now able demonstrate...
Plants have developed an internal timing mechanism, the circadian system, that serves to synchronise physiological and metabolic functions with daily cues such as dawn dusk, provides plants advantage in adapting changing challenging conditions. We recently shown SAL1-PAP-XRN retrograde signalling pathway, which is proposed regulate plant responses under stress conditions, also acts within system. Here we provide further evidence of regulation by signalling, thereby affirming a link between...
Society Impact Statement Until the 1970s, majority of commercial strawberry varieties produced a single bloom flowers. However, continuously flowering, everbearing strawberries are now routinely cultivated and use is increasing. Indeed, introgression flowering trait can lead to economic benefits for growers through production continual crop from same plant. Genetically guided improvement has power streamline generation. As such, genetic markers reported here help identify individuals at an...
Summary Previous work within the community led to identification of a single dominant allele that controls everbearing trait. However, frequent observations have indicated crosses do not segregate in Mendelian fashion, as would be expected for trait controlled by gene. Therefore, it was hypothesised one or more unidentified epistatic alleles interact with major A GWAS conducted on 587 June-bearers and 207 everbearers assess genetic components associated flowering habit. The segregation...
Abstract The circadian clock system acts as an endogenous timing reference that coordinates many metabolic and physiological processes in plants. Previous studies have shown the application of osmotic stress delays rhythms via 3’-Phospho-Adenosine 5’-Phosphate (PAP), a retrograde signalling metabolite is produced response to redox within organelles. PAP accumulation leads inhibition EXORIBONUCLEASEs (XRNs), which are responsible for RNA degradation. Interestingly, we now able demonstrate...