A5086863073- Rabies epidemiology and control
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Retinal Development and Disorders
- Mosquito-borne diseases and control
- Bacillus and Francisella bacterial research
- Venomous Animal Envenomation and Studies
- Viral Infections and Outbreaks Research
- Prion Diseases and Protein Misfolding
- Advanced MRI Techniques and Applications
- Bariatric Surgery and Outcomes
- Lipid Membrane Structure and Behavior
- Epilepsy research and treatment
- Reading and Literacy Development
- Virus-based gene therapy research
- Neurobiology of Language and Bilingualism
- Functional Brain Connectivity Studies
- Pharmacological Effects and Toxicity Studies
- Neurogenetic and Muscular Disorders Research
- Cellular transport and secretion
- Poxvirus research and outbreaks
- Amyotrophic Lateral Sclerosis Research
McGovern Institute for Brain Research
2018-2024
Massachusetts Institute of Technology
2018-2024
Regional Medical Center
2019
University of Maine
2017
Augusta University Health
2003
Augusta University
2002
Abstract Rabies-virus-based monosynaptic tracing is a widely used technique for mapping neural circuitry, but its cytotoxicity has confined it primarily to anatomical applications. Here we present second-generation system labeling direct inputs targeted neuronal populations with minimal toxicity, using double-deletion-mutant rabies viruses. Viral spread requires expression of both deleted viral genes in trans postsynaptic source cells. Suppressing this doxycycline following an initial period...
Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to starting neuronal population. Its most common implementation is use Cre mouse lines combination with Cre-dependent "helper" adeno-associated viral vectors (AAVs) supply the required genes targeted population before subsequent injection first-generation (∆G) vector. Here we...
Monosynaptic tracing using rabies virus is an important technique in neuroscience, allowing brain-wide labeling of neurons directly presynaptic to a targeted neuronal population. A 2017 article reported the development noncytotoxic version-a major advance-based on attenuating by addition destabilization domain C terminus viral protein. However, this modification did not appear hinder ability spread between neurons. We analyzed two viruses provided authors and show here that both were mutants...
Rabies viral vectors have become important components of the systems neuroscience toolkit, allowing both direct retrograde targeting projection neurons and monosynaptic tracing inputs to defined postsynaptic populations, but rapid cytotoxicity first-generation (ΔG) limits their use short-term experiments. We recently introduced second-generation, double-deletion-mutant (ΔGL) rabies vectors, showing that they efficiently retrogradely infect express recombinases effectively with little no...
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder leading to progressive paralysis and death within 2–5 years after diagnosis. Sporadic cases (SALS) comprise approximately 90% of with the remaining 10% familial (FALS) caused by mutations in 27 genes. The vast heterogeneity seen age location disease onset, rate progression, duration has been linked genetic environmental influences both SALS FALS cases. Increased ALS incidence clusters Guam, southern France, Maryland have...
SUMMARY Rabies viral vectors have become important components of the systems neuroscience toolkit, allowing both direct retrograde targeting projection neurons and monosynaptic tracing inputs to defined postsynaptic populations, but rapid cytotoxicity first-generation (ΔG) limits their use short-term experiments. We recently introduced second-generation, double-deletion-mutant (ΔGL) rabies vectors, showing that they efficiently retrogradely infect express recombinases effectively with little...
ABSTRACT Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to starting neuronal population. Its most common implementation is use Cre mouse lines combination with Cre-dependent “helper” adeno-associated viral vectors (AAVs) supply the required genes targeted population before subsequent injection first-generation (ΔG)...
Abstract Monosynaptic tracing is a widely-used technique for mapping neural circuitry, but its cytotoxicity has confined it primarily to anatomical applications. Here we present second-generation system labeling direct inputs targeted neuronal populations with minimal toxicity, using double-deletion-mutant rabies viruses. Spread of the viruses requires expression both deleted viral genes in trans postsynaptic source cells; suppressing this doxycycline following an initial period replication...
ABSTRACT Monosynaptic tracing using rabies virus is an important technique in neuroscience, allowing brain- wide labeling of neurons directly presynaptic to a targeted neuronal population. A 2017 article reported development noncytotoxic version – major advance based on attenuating the by addition destabilization domain C-terminus viral protein. However, this modification did not appear hinder ability spread between neurons. We analyzed two viruses provided authors and show here that both...