An alkaline protease producer Bacillus sp. strain CA15 was isolated from soil. The microorganism found to be closely related cereus based on 16S ribosomal DNA sequencing. culture conditions for higher production were optimized with respect carbon and nitrogen sources, metal ions, pH temperature. Maximum obtained in the medium supplemented 1% skim milk, starch 0.6% MgSO 4 .7H 2 O, initial 8.0 at 35°C. best enzyme during stationary phase which cell density reached 1.8x108 cells/mL. level of...

The production of extracellular alkaline protease by producing Bacillus subtilis RSKK96 was studied with solid state fermentation (SSF). Different agro residues as substrate were for enzyme production. highest expressed lentil husk units per mass dry (3937.0 U/mg). Production parameters optimized incubation time 120 h, extraction medium Triton-X100 1%, initial moisture content 30%, pH 9.0. high level obtained in the containing arabinose followed lactose, galactose, and fructose. Among...

Abstract Production of alkaline α‐amylase employing our laboratory isolate, Bacillus sp., under solid state fermentation, was optimized. The effect wheat bran and lentil husk examined. Lentil exhibited the highest enzyme production. appropriate incubation time, inoculum size, moisture level, buffer solution level were determined. Maximum yields 216,000 172,800 U/g achieved by as substrates in 0.1 M carbonate/bicarbonate at pH 10.0 with 30% initial 24 h. Inoculum size found to be 20% 1:0.5...

In this study, the Taguchi experimental design was applied to optimize conditions for α-amylase production by Bacillus subtilis RSKK96, which purchased from Refik Saydam Hifzissihha Industry (RSHM). Four factors, namely, carbon source, nitrogen amino acid, and fermentation time, each at four levels, were selected, an orthogonal array layout of L16 (45) performed. The model equation obtained validated experimentally maximum casein (1%), corn meal glutamic acid (0.01%) concentrations with...

AbstractPistacia terebintus, a member of the family Anacardiaceae, is perennial plant that widely grows in southern and western regions Anatolia. Pistacia terebintus L. seeds contain 66% oil, which allows possibility economical exploitation. The main monounsaturated fatty acid oleic (55–75% w/w), polyunsaturated linoleic (15–38% while saturated palmitic (8–20% w/w). seed oil was investigated as an alternative feedstock for production biodiesel fuel. Three commonly used catalysts...

Bacillus subtilis was grown in media containing various amino acids. The effects of these acids on the production secreted α-amylase and membrane-bound were investigated. Their transport through membranes also determined. synthesis rate isolated from bacterial stimulated by growth separately Ala Arg, all other being inhibitory. Cys, Arg Leu increased membrane, while Ala, Asp Ser inhibited it. Secreted activity appreciably Arc, Gln, Gly, Leu, Phe, Pro, especially Cys it repressed Asp, Asn,...

Aim: Adherent-invasive Escherichia coli (AIEC) pathovar has been identified in intestinal mucosa of patients with Crohn's disease. Our aim was to compare the impact sterile mucosal media (Muc-M) originated from different parts intestine on some pathogenic traits AIEC LF82 strain. Materials & methods: Muc-M composed certain rates cell culture medium or M63 minimal and contents obtained part were designed for cell-infection experiments biofilm-formation assays. Results: The results showed that...

The bacterial strain Bacillus subtilis RSKK96 was shown to produce extracellular α-amylase. Enzyme synthesis occurred at 72 h with an optimum of 37°C. Eff ects various carbon, nitrogen, amino acid sources and chemicals on α-amylase production were examined. FeSO4, ZnSO4 CuSO4 inhibited growth as a result, amylase production. Maximum (858.6±41.9 U/ mg) obtained in medium containing 0.5% cotton stalk h.

The usages of enzymes are used in biotechnology, industry, food, textil, paper, medicine and pharmaceutical. Enzymes obtained from microorganisms produced by have some advantages when compared with plants or animals, they considerably higher catalytic activity, don’t undesirable by-products, more stable relatively cheap, can be much quantity. main goal the present study is to realize optimization parameters α-amylase Bacillus subtilis ATCC 6051 solid phase fermentation method. best...

Objective:The aim of this study was to investigate the effect healthy mucosa on adhesive and invasive properties AIEC reference strain Escherichia coli (E.coli) LF82.For purpose, we had designed special medias that contained cell culture medium mucosal content obtained from different regions (colon ileum) digestive tract. Methods:We tested infecting ability E. LF82 I-407 cells in presence media (Muc-M) under vitro conditions.Muc-M composed certain rates or M63 minimal contents part intestine...