Myceliophthora thermophila is a thermophilic fungus whose genome encodes wide range of carbohydrate-active enzymes (CAZymes) involved in plant biomass degradation. Such have potential applications turning different kinds lignocellulosic feedstock into sugar precursors for biofuels and chemicals. The present study examined compared the transcriptomes exoproteomes M. during cultivation on types complex to gain insight how its secreted enzymatic machinery varies with sources lignocellulose. In...

Although protein carbonyl formation is an index of oxidative stress in skeletal muscles, the exact proteins, which undergo oxidation these remain unknown. We used 2D electrophoresis, immunoblotting, and mass spectrometry to identify carbonylated proteins diaphragm septic animals. Rats were injected with saline (control) or Escherichia coli lipopolysaccharides (LPS) killed after various intervals. Diaphragm carbonylation increased significantly peaked 12 h LPS injection, it was localized both...

The ability to obtain carbon and energy is a major requirement exist in any environment. For several ascomycete fungi, (post-)genomic analyses have shown that species occupy large variety of habitats possess diverse enzymatic machinery, while with specific habitat more focused enzyme repertoire well-adapted the prevailing substrate. White-rot basidiomycete fungi also live habitat, as they are found exclusively wood. In this study, we evaluated how well machinery white-rot fungus Dichomitus...

Proteases can hydrolyze peptides in aqueous environments. This property has made proteases the most important industrial enzymes by taking up about 60% of total enzyme market. Microorganisms are main sources for protease production due to their high yield and a wide range biochemical properties. Several Aspergilli have ability produce variety proteases, but no comprehensive comparative study been carried out on productivity this genus so far. We performed combined analysis genomics,...

The transcription factor GaaR is needed for the expression of genes required pectin degradation and transport catabolism main product, D-galacturonic acid (GA) in Aspergillus niger. In this study, we used strong constitutive gpdA promoter nidulans to overexpress gaaR A. Overexpression resulted an increased encoding pectinases, (putative) GA transporters, catabolic pathway enzymes even under non-inducing conditions, i.e., absence GA. Exoproteome analysis a strain overexpressing showed that...

Abstract Background The ascomycete fungus Podospora anserina has been appreciated for its targeted carbohydrate-active enzymatic arsenal. As a late colonizer of herbivorous dung, the acts specifically on more recalcitrant fraction lignocellulose and this lignin-rich biotope might have resulted in evolution ligninolytic activities. However, lignin-degrading abilities not demonstrated by chemical analyses at molecular level are, thus far, solely based genome secretome predictions. To evaluate...

Microbial lytic polysaccharide monooxygenases (LPMOs) cleave diverse biomass polysaccharides, including cellulose and hemicelluloses, by initial oxidation at C1 or C4 of glycan chains. Within the Carbohydrate-Active Enzymes (CAZy) classification, Auxiliary Activity Family 9 (AA9) comprises first largest group fungal LPMOs, which are often also found in tandem with non-catalytic carbohydrate-binding modules (CBMs). LPMOs originally attracted attention for their ability to potentiate complete...

The extent of carbon catabolite repression (CCR) at a global level is unknown in wood-rotting fungi, which are critical to the cycle and source biotechnological enzymes. CCR occurs presence sufficient concentrations easily metabolizable sources (e.g., glucose) involves downregulation expression genes encoding enzymes involved breakdown complex sources. We investigated this phenomenon white-rot fungus Dichomitus squalens using transcriptomics exoproteomics. In D. cultures, approximately 7%...

Penicillium subrubescens is able to degrade a broad range of plant biomass and it has an expanded set Carbohydrate Active enzyme (CAZyme)-encoding genes in comparison other species. Here we used exoproteome transcriptome analysis demonstrate the versatile degradation mechanism by P. during growth on wheat bran sugar beet pulp. On degraded xylan main chain side residues from Day 2 cultivation, whereas started chains pectin pulp prior attacking 3. In addition, 3 cellulolytic enzymes were...

The unique ability of basidiomycete white rot fungi to degrade all components plant cell walls makes them indispensable organisms in the global carbon cycle. In this study, we analyzed proteomes two closely related fungi, Obba rivulosa and Gelatoporia subvermispora, during eight-week cultivation on solid spruce wood. Plant wall degrading carbohydrate-active enzymes (CAZymes) represented approximately 5% total proteins both species. A core set orthologous CAZymes was shared between these...

We investigated the identity and quantitative variations of proteins extracted from human sperm heads using a label-free Gel-MS approach. Sperm samples were obtained three men with high counts at different time points. This design allowed us to analyse intra-individual inter-individual head proteome. Each point was analyzed in triplicate minimize any background artifactual effects methodology on variation analyses. Intra-individual analysis spectral counting method revealed that expression...

Tannic acid, a hydrolysable gallotannin present in plant tissues, consists of central glucose molecule esterified with gallic acid molecules. Some microorganisms, including several Aspergillus species, can metabolize tannic by releasing residues from secreting specific esterases into the medium. The expression these so-called tannases is induced or acid. In this study, we identified conserved transcriptional activator-repressor module involved regulation predicted and other genes metabolism....

Summary Small secreted proteins ( SSP ) have been defined as containing a signal peptide and sequence of less than 300 amino acids. In this analysis, we compared the secretion pattern s among eight aspergilli species in context plant biomass degradation highlighted putative interesting candidates that could be involved degradative process or strategies developed by fungi to resist associated stress due toxicity some aromatic compounds reactive oxygen released during degradation. Among these...

Previously, DNA microarrays analysis showed that, in co-culture with Bacillus subtilis, a biosynthetic gene cluster anchored nonribosomal peptides synthetase of Aspergillus niger is downregulated. Based on phylogenetic and synteny analyses, we show here that this cluster, NRRL3_00036-NRRL3_00042, comprises genes predicted to encode synthetase, FAD-binding domain-containing protein, an uncharacterized transporter, cytochrome P450 NAD(P)-binding protein transcription factor. We overexpressed...

Summary Fungi produce a wide range of enzymes that allow them to grow on diverse plant biomass. Wheat bran is low‐cost substrate with high potential for biotechnological applications. It mainly contains cellulose and (arabino)xylan, as well starch, proteins, lipids lignin lesser extent. In this study, we dissected the regulatory network governing wheat degradation in Aspergillus niger assess relative contribution regulators utilization biomass substrate. Deletion genes encoding transcription...