A5105873165- Escherichia coli research studies
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Epigenetics and DNA Methylation
- Evolution and Genetic Dynamics
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Legume Nitrogen Fixing Symbiosis
- Streptococcal Infections and Treatments
- Oral microbiology and periodontitis research
- Neonatal and Maternal Infections
- Urinary Tract Infections Management
National University of Singapore
2015-2020
National University Health System
2015-2020
National Environment Agency
2017
Genome Institute of Singapore
2017
Agri-Food and Veterinary Authority of Singapore
2017
Nanyang Technological University
2017
Singapore General Hospital
2017
Creation of defined genetic mutations is a powerful method for dissecting mechanisms bacterial disease; however, many tools are only developed laboratory strains. We have designed modular and general negative selection strategy based on inducible toxins that provides high stringency in clinical Escherichia coli Salmonella isolates. No strain- or species-specific optimization needed, yet this system achieves better than all previously reported systems usable unmodified E. The enables use...
Abstract We assessed microbial safety and quality of raw fish sold in Singapore during 2015–2016 to complement epidemiologic findings for an outbreak infection with group B Streptococcus serotype III sequence type (ST) 283 associated consumption. Fish-associated ST283 strains included nearly identical (0–2 single-nucleotide polymorphisms) the human strain, as well another distinct clade (57–71 polymorphisms). Our investigations highlight risk contamination freshwater (which are handled...
Fluorescent proteins, especially green fluorescent protein (GFP), have been instrumental in understanding urinary tract infection pathogenesis by uropathogenic Escherichia coli (UPEC). We used a recently developed GFP variant, vsfGFP-9, to create new plasmid- and chromosome-based derivatives of the UPEC strain UTI89. The vsfGFP-9 strains are nearly 10× brighter with no vitro growth or vivo virulence defects compared previously reported GFP-expressing UTI89 strains. chromosomal is equivalent...
Escherichia coli is the most well-studied bacterium and a common colonizer of lower mammalian gastrointestinal tract. We report here complete genome sequence original isolate, strain NCTC86, which was described by Theodor Escherich, for whom genus named.
DNA methylation is a common epigenetic mark that influences transcriptional regulation, and therefore cellular phenotype, across all domains of life. In particular, both orphan methyltransferases those from phasevariable restriction modification systems (RMSs) have been co-opted to regulate virulence epigenetically in many bacteria. We now show three distinct non-phasevariable Type I RMSs Escherichia coli no measurable impact on gene expression, vivo virulence, or any 1190 vitro growth...
The availability of matched sequencing data for the same sample across different platforms is a necessity validation and effective comparison platforms. A commonly sequenced lab-adapted MG1655 strain Escherichia coli; however, this not fully representative more complex dynamic genomes pathogenic E. coli strains. We present six new sets another strain, UTI89, which an extraintestinal isolated from patient suffering urinary tract infection. now provide whole genome generated using PacBio RSII,...
Studies of Uropathogenic Escherichia coli (UPEC) pathogenesis have relied heavily on genetic manipulation to understand virulence factors. We applied a recently reported positive-negative selection system create series unmarked, scarless FimH mutants that show identical phenotypes previously marked mutants; these are now improved versions useful for definitive assignment mutations. also increased the efficiency this by designing new primer sites, which should further improve and convenience...
Abstract DNA methylation is a common epigenetic mark that influences transcriptional regulation, and therefore cellular phenotype, across all domains of life, extending also to bacterial virulence. Both orphan methyltransferases those from restriction modification systems (RMSs) have been co-opted regulate virulence epigenetically in many bacteria. However, the potential regulatory role mediated by archetypal Type I Escherichia coli has never studied. We demonstrated removal methylated three...