Recent studies suggest that chronic infections including those associated with periodontitis increase the risk for coronary vascular disease (CVD) and stroke. We hypothesize oral microorganisms periodontal bacterial pathogens enter blood stream during transient bacteremias where they may play a role in development progression of atherosclerosis leading to CVD.To test this hypothesis, 50 human specimens obtained carotid endarterectomy were examined presence Chlamydia pneumoniae,...

Previous studies suggest differences between geographically and racially distinct populations in the prevalence of periodontopathic bacteria as well greater periodontal destruction associated with infection by highly leucotoxic Actinobacillus actinomycetemcomitans. The present study examined these hypotheses Brazilians aggressive or chronic periodontitis.Clinical, radiographical, microbiological assessments were performed on 25 periodontitis 178 patients including 71 males 132 females, 15-69...

This study evaluated the antimicrobial activity of two commercially available 0·05% cetylpyridinium chloride (CPC) mouthrinses with or without alcohol and examined its on oral bacterial species including fresh clinical isolates compared to a chlorhexidine mouthrinse control fluoride CPC. Two different approaches were used evaluate activity. First, minimum inhibitory concentration (MIC) was determined for each against panel 25 micro-organisms associated dental caries, gingivitis...

Actinobacillus actinomycetemcomitans leukotoxin is thought to be an important virulence factor in the pathogenesis of localized juvenile and other forms early-onset periodontitis. Some highly leukotoxic A. strains produce 10 20 times more than minimally strains. The distribution, clonality, intrafamilial transmission were examined order determine importance periodontitis.The polymerase chain reaction (PCR) was used differentiate from examining 1,023 fresh isolates our culture collection....

R ecent studies of the dental plaque bacteria associated with various forms early‐onset periodontitis confirm importance target periodontal pathogens such as Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Prevotella intermedia , and Porphyromonas gingivalis in these diseases. A. actinomycetemcomitans strains exhibit a wide range variability leukotoxin production. By virtue unique promoter for ( ltx ) operon, highly leukotoxic (e.g., JP2) express 10‐ to 20‐times greater levels...

Background: The aim of the present study is to evaluate use anorganic bovine bone (ABB) associated with a collagen membrane (CM) for sinus graft by means clinical, histologic, and radiographic parameters in cases availability ≤7 mm. A preliminary evaluation consisted clinical examination, computed tomography (CT), panoramic x‐ray. Methods: Ninety‐two patients requiring bilateral grafts 222 unilateral procedures (total: 406 sinuses) participated this study. total 1,025 implants were placed...

Previous studies suggest that Solobacterium moorei is associated with oral halitosis. In the present study, we examined prevalence of S. on dorsal surface tongue in 57 adults (21 and 36 without halitosis) by bacterial culture direct amplification nucleic acids. We also type strain four clinical isolates for 16S ribosomal acid sequence, H(2)S enzyme production, antibiotic susceptibility. was found 100% subjects halitosis 14% Infection correlated organoleptic measures volatile sulfur compound...

Sreenivasan PK, Vered Y, Zini A, Mann J, Kolog H, Steinberg D, Zambon JJ, Haraszthy VI, da Silva MP, De Vizio W. A 6-month study of the effects 0.3% triclosan/copolymer dentifrice on dental implants. J Clin Periodontol 2011; 38: 33–42. doi: 10.1111/j.1600-051X.2010.01617.x. Aim: Supportive therapy to maintain implants is increasingly important. This examined effect a triclosan/2% copolymer oral biofilms and gingival inflammation (GI) peri-implant tissues. Materials Methods: One hundred...

This study compared the additional effect of rinsing with a fluoride-free and alcohol-free 0.075% cetylpyridinium chloride (CPC) mouthwash to brushing alone on dental plaque, gingival inflammation, supragingival plaque bacteria.Adult subjects [n = 68] completed washout period prior baseline evaluations that evaluated bleeding, pocket probing depths along microbiological analysis for bacteria. Subjects were randomized two treatment groups: brush fluoride toothpaste rinse CPC (test) or only...

This study examined the prevalence of highly and minimally leukotoxic Actinobacillus actinomycetemcomitans in patients with periodontal disease. Pooled subgingival plaque samples from 136 some form disease were examined. Subjects between 14 76 years age. Clinical examinations included pocket depth (PD), index (PI) bleeding (BI). The obtained for presence or A. strains by polymerase chain reaction (PCR). Chi-square logistic regression performed to evaluate results. Forty-seven subjects...

The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers the polymerase chain reaction. Genomic from each 20 human isolates A. was successfully amplified thermal cycler with single primer (GGGTAACGCC) and reproducibly produced 14 different amplification profiles (amplitypes). same subject revealed amplitype. arbitrarily primed reaction appears to be useful characterizing studies...

To determine minimal inhibitory concentrations (MICs) and the percentage of nonsusceptible bacteria-- those still cultivable above a threshold concentration--in human supragingival dental plaque saliva for antiplaque/antimicrobial agents including triclosan (TCS) trichlorocarbanilide (TCC), new potential antimicrobial, 2-t-butyl-5-(4-t-butylphenyl)-phenol (DTBBP).Broth agar dilution-based MIC tests were performed using 28 oral nonoral bacterial strains representing 17 species. MICs TCS...

Actinobacillus actinomycetemcomitans is an oral pathogen that causes aggressive periodontitis as well sometimes life-threatening, extra-oral infections. Iron regulation thought to be important in the pathogenesis of A. infections and, consistent with this hypothesis, fur gene has recently been identified and characterized actinomycetemcomitans. In study, 14 putatively Fur-regulated genes were by Fur titration assay (Furta) actinomycetemcomitans, including afuA, dgt, eno, hemA, tbpA, recO yfe...

We report the genome sequences of three clinical isolates Tannerella forsythia from subgingival plaque periodontitis patients attending clinics at School Dental Medicine, University Buffalo. The availability these will aid understanding pathogenesis periodontitis.

The purpose of this study was to compare biofilm formation on materials used for the fabrication implant-supported dental prostheses. Twenty discs (D=15 mm, H=3 mm) were fabricated from one following restorative materials: yttria tetragonal zirconia polycrystal (Y-TZP); commercially pure titanium (CP-Ti); or heat-cured polymethyl methacrylate (PMMA). Specimens polished standard protocols. A non-contact profilometer (NPFLEX, Bruker, UK) assess surface roughness each disk; results reported as...

Actinobacillus actinomycetemcomitans is a Gram-negative coccobacillus which can cause certain severe extra-oral infections as well forms of human periodontal disease such localized juvenile periodontitis. In contrast to many prokaryotic and eukaryotic species exhibit an intact 23S ribosomal RNA (rRNA) molecule, examination six A. strains-including three serogroup representative strains two from non-human primates-revealed that this micro-organism does not produce molecule but, rather,...

Actinobacillus actinomycetemcomitans is a key microorganism in the pathogenesis of several different forms periodontal diseases. Identification this bacterium from clinical specimens may often be complicated by fact that colony morphology on TSBV selective medium closely resembles Haemophilus aphrophilus and differentiating characteristic, catalase reaction, variable. Recent genetic studies have shown 23S ribosomal RNA molecule split into two smaller A. , but intact H. aphrophilus. Based...