A5017912718- Mitochondrial Function and Pathology
- Nicotinic Acetylcholine Receptors Study
- Endoplasmic Reticulum Stress and Disease
- Redox biology and oxidative stress
- Diffusion Coefficients in Liquids
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- NMR spectroscopy and applications
- Vitamin C and Antioxidants Research
- Air Quality and Health Impacts
- Material Dynamics and Properties
- Glycosylation and Glycoproteins Research
- Behavioral Health and Interventions
- Biochemical effects in animals
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Lipid Membrane Structure and Behavior
- Neural and Behavioral Psychology Studies
- Adenosine and Purinergic Signaling
- Carcinogens and Genotoxicity Assessment
- Chronic Obstructive Pulmonary Disease (COPD) Research
- ATP Synthase and ATPases Research
- Reproductive System and Pregnancy
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2016-2021
Polish Academy of Sciences
2017-2021
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2016
Poznan University of Medical Sciences
2013
Wojskowy Instytut Medycyny Lotniczej
2012
In patients with Ovarian Cancer (OvCa) exosomes released by tumor cells are present in the plasma and could be involved progression. This study examines association between exosome presence/protein content of OvCa disease outcome, response to standard therapy and/or tumorresistance therapies studied at diagnosis also serially during after therapy.Exosomes were purified from patients' (n=22), benign tumors (n=10) or healthy controls (NC) using ultracentrifugation. Exosomes visualized scanning...
Metabolic reactions in living cells are limited by diffusion of reagents the cytoplasm. Any attempt to quantify kinetics biochemical cytosol should be preceded careful measurements physical properties cellular interior. The cytoplasm is a complex, crowded fluid characterized effective viscosity dependent on its structure at nanoscopic length scale. In this work, we present and validate model describing cytoplasmic nanoviscosity, based seven human cell lines, for nanoprobes ranging diameters...
Mitochondrial dysfunction caused by cigarette smoke is involved in the oxidative stress-induced pathology of airway diseases. Reducing levels harmful and potentially constituents heating rather than combusting tobacco may reduce mitochondrial changes that contribute to stress cell damage. We evaluated function human bronchial epithelial cells (BEAS 2B) following 1- 12-week exposures total particulate matter (TPM) from aerosol a candidate modified-risk product, Tobacco Heating System 2.2...
This work, based on in vivo and vitro measurements, as well silico simulations, provides a consistent analysis of diffusion polydisperse nanoparticles the cytoplasm living cells. Using example fluorescence correlation spectroscopy (FCS), we show effect polydispersity probes experimental results. Although individual undergo normal diffusion, ensemble probes, an effective broadening distribution times occurs—similar to anomalous diffusion. We introduced fluorescently labeled dextrans into HeLa...
One of the main players in process mitochondrial fragmentation is dynamin-related protein 1 (Drp1), which assembles into a helical ring-like structure on mitochondria and facilitates fission. The fission mechanism still poorly understood detailed information concerning oligomeric form Drp1, its cellular distribution size complex missing. To estimate forms Drp1 cytoplasm mitochondria, we performed quantitative analysis diffusion gene-edited HeLa cell lines. This paper provides an insight...
Biochemistry in living cells is an emerging field of science. Current quantitative bioassays are performed ex vivo, thus equilibrium constants and reaction rates reactions occurring human still unknown. To address this issue, we present a non-invasive method to quantitatively characterize interactions (equilibrium constants, KD) directly within the cytosol cells. We reveal that cytosolic hydrodynamic drag depends exponentially on probe's size, provide model for its determination different...
Mitochondria are among the first responders to various stress factors that challenge cell and tissue homeostasis. Various plant alkaloids have been investigated for their capacity modulate mitochondrial activities. In this study, we used isolated mitochondria from mouse brain liver tissues assess nicotine, anatabine anabasine, three found in tobacco plant, potential modulatory activity on bioenergetics parameters. All decreased basal oxygen consumption of a dose-dependent manner without any...
Abstract Background: Ectonucleotidases CD39/CD73 have been reported to play an important role in functional supression of various immune cells via adenosine that is generated locally the tumor microenvironment. In patients with ovarian cancer (OvCa) exosomes released by (TEX) are abundant body fluids, including plasma or ascites and may be involved progression. Based on observations suggesting TEX carry proteins expressed from which originate, we hypothesized CD39+ CD73+ could deliver these...