In fermented foods, including kimchi, salinity is a crucial factor that influences preservation and quality. We aimed to investigate the physicochemical characteristics, microbiota, metabolites of kimchi at varying salinities over 200 days. Kimchi cabbages were soaked in 3%, 10%, 20% (w/v) saline solutions for 10 h prepare low-, middle-, high-salinity respectively. Although level had significant impact on its appearance, microbial community, metabolite content early stages (until 5th day),...

Garlic generally inhibits the growth of microorganisms; however, mechanism underlying its selective impact on various kimchi microorganisms is unknown. We investigated effects garlic microbial community and metabolites in during fermentation. Difference color white colony-forming yeast were found only without 100th day The showed a relatively high proportion Lactobacillus, but low Leuconostoc. levels homoserine correlated with initial quantities added. Meanwhile, 11 metabolites, including...

Starch paste, one of the ingredients used in kimchi, facilitates fermentation by acting as a nutrient source for microorganisms during process. Changes microbial communities and metabolites were investigated to understand effects starch with varying amylose contents on kimchi fermentation. Although adding paste changed profile there was no significant difference content. In paste-added Leuconostoc, which is mainly involved fermentation, found be relatively more abundant than without starch....

This study aimed to examine the impacts of essential and optional ingredients on microbial metabolic profiles kimchi during 100 days fermentation, using a mix-omics approach. Kimchi manufactured without (e.g., red pepper, garlic, ginger, green onion, radish) had lower lactic acid content. The absence garlic was associated with higher proportion

Halitosis is mainly caused by the action of oral microbes. The purpose this study was to investigate differences in salivary microbes and metabolites between subjects with without halitosis. Of 52 participants, 22 were classified into halitosis group volatile sulfur compound analysis on breath samples. 16S rRNA gene amplicon sequencing metabolomics approaches used difference saliva control groups. profiles microbiota relatively different relative abundances Prevotella, Alloprevotella,...

In this study, green coffee beans were fermented using three yeast and lactic acid bacteria (LAB) starters. The effects of each starter on the metabolite profiles comparatively analyzed. principal component analysis (PCA) biplot showed a clear separation between groups with LAB (Leuconostoc mesenteroides, Lactobacillus plantarum, Pediococcus pentosaceus) (Saccharomyces cerevisiae, Candida parapsilosis, Pichia guilliermondii). Various metabolites, including palmitic acid, stearic piperidine,...

Accumulated clinical and biomedical evidence indicates that the gut microbiota their metabolites affect brain function behavior in various central nervous system disorders. This study was performed to investigate changes composition of fecal microbial community following injection amyloid β (Aβ) donepezil treatment Aβ-injected mice using metataxonomics metabolomics. Aβ caused cognitive dysfunction, while resulted successful recovery memory impairment. The + group showed a significantly...

Highlights•Garlic content changed fermentation modality by affecting growth of lactic acid bacteria.•The garlic selectively affected specific bacterial taxa and metabolites.•Individual LAB strains inoculated with the same exhibited a strain-dependent metabolite production.AbstractGarlic is an essential ingredient added to kimchi, fermented vegetable, in small amounts owing its sensory antibacterial properties. This study aimed elucidate complex relationship between bacteria (LAB) resulting...

The purpose of this study was to analyze metabolic differences ginseng berries according cultivation age and ripening stage using gas chromatography-mass spectrometry (GC-MS)-based metabolomics method. Ginseng were harvested every week during five different stages three-year-old four-year-old ginseng. Using identified metabolites, a random forest machine learning approach applied obtain predictive models for the classification or stage. Principal component analysis (PCA) score plot showed...

Age-related gut microbes and urine metabolites were investigated in 568 healthy individuals using metataxonomics metabolomics. The richness evenness of the fecal microbiota significantly increased with age, abundance 16 genera differed between young old groups. Additionally, 17 contributed to differences Among that by Bacteroides Prevotella 9 confirmed be correlated some metabolites. machine learning algorithm eXtreme gradient boosting (XGBoost) was shown produce best performing age...

In this study, changes in physicochemical characteristics, microbial communities, and metabolites were investigated to identify the fermentation characteristics of radish kimchi according size cubes used. The small-sized group showed highest hardness value glucose content early stages fermentation. relative abundance major lactic acid bacteria, including Leuconostoc, Weissella, Lactobacillus, was on day 5 fermentation, which resulted rapid production acid, thereby causing a decrease pH an...

The purpose of this study is to investigate the effect fungi on kimchi metabolites during fermentation. A gas chromatography-mass spectrometry (GC-MS) based metabolite profiling approach in combination with principal component analysis (PCA) performed differentiate produced by or bacteria. To avoid bacterial growth, treated 100 μg/mL ampicillin every three days from 30 50 relative content major at fermentation, between control group and treatment group, was not significantly different....

Sasang constitutional medicine classifies human beings into four types based on their physical and psychological characteristics. Despite its potential value in achieving personalized medicine, the diagnosis of sasang constitution (SC) type is complex subjective. In this study, gas chromatography–mass spectrometry 1 H nuclear magnetic resonance–based metabolic analyses were conducted to find maker metabolites serum urine according different SC types. Although some samples overlapped...

Accurate prediction of failure to progress and rapid decision making regarding the mode delivery can improve pregnancy outcomes. We examined value sonographic cervical markers in successful vaginal beyond 34 weeks gestation. A retrospective chart review was carried out. Medical information singleton gestations delivered at a single center from 1 July 2019 30 August 2020 collected. Transvaginal records length, anterior posterior angles, dilatation were obtained re-measured. The these clinical...

BACKGROUND. Long acquisition times for breath-hold contrast-enhanced (CE) T1-weighted imaging in MR enterography (MRE) protocols result reduced image quality. OBJECTIVE. The purpose of this study was to compare CE performed using sensitivity encoding (SENSE) and compressed sensing-SENSE (CS-SENSE) terms quality diagnostic performance active inflammation Crohn disease (CD). METHODS. This retrospective included 41 patients (31 men, 10 women; mean age, 34 ± 12 [SD] years) who underwent MRE...

The purpose of this study was to understand the changes metabolic pathway induced by alpha-melanocyte-stimulating hormone (α-MSH) in B16F10 melanoma cells an untargeted metabolomics approach. Cells were treated with 100 nM α-MSH and then incubated for 48 h. increased tyrosinase activity melanin content 56.5 61.7%, respectively, compared untreated after h cultivation. clear separation between groups observed principal component analysis score plot, indicating that levels metabolites altered...

Objectives : It was conducted to experimentally analyze the effects of acupuncture treatment at CV12, ST25, and ST36 on weight, FBCS, fat metabolism, microbiome, metabolome changes in general diet rat high-fat rat.Methods classified into four groups: & non-treatment group (ND), (ND+AT), (HFD), (HFD-AT).After performed ST36, body were analyzed.Results Compared ND group, ND+AT had no significant effect.Compared HFD HFD+AT reduced inflammatory cytokine IL-6 expression, lipid droplet...

Multiple organ invasion by keratinophilic fungi in the green iguana (Iguana iguana) has not been previously reported. In this case, a 1-yr-old female presented with nodular, darkly discolored skin lesion surrounded necrosis right ventral abdominal region. A cytologic examination of fine needle aspiration revealed an exuberant proliferation fibroblasts, macrophages, and multinucleated cells along frequent filamentous structures consistent hyphal elements. The necropsy diffuse infiltration...

GC/MS coupled with multivariate statistical analysis was performed to identify marker metabolites in serum of mice after healing ovalbumin- (OVA-) induced asthma using Opuntia humifusa . Principal component (PCA) score plot showed separation among groups, metabolite profiles showing differences according various treatments for the murine model. Levels stearic acid and arachidic were significantly lower from OVA-induced group than those control group. Dexamethasone treatment characterized by...