Endothelial dysfunction has been suggested as a possible causal link between hyperglycemia and microvascular complications in diabetes mellitus. The effect of metformin on endothelial progenitor cells (EPCs) is still unclear. This study was designed to test the hypothesis that could accelerate wound healing by improving impaired EPC functions streptozotocin-induced diabetic mice. Streptozotocin (STZ, 60 mg/kg/d × 5 d, i.p.) injected induce type 1 male C57BL/6 Mice were treated with (250...

Wound healing impairment is increasingly recognized to be a consequence of hyperglycemia‑induced dysfunction endothelial precursor cells (EPCs) in type 2 diabetes mellitus (T2DM). Metformin exhibits potential for the improvement function and wound process. However, underlying mechanisms observed beneficial effects metformin application remain completely understood. The present study assessed whether metformin, widely used therapeutic drug T2DM, may accelerate closure T2DM db/db mice....

Metformin is currently the most widely used first-line hypoglycemic agent for diabetes mellitus. Besides glucose-lowering action, there increasingly interest in potential anti-inflammatory action of this drug. In present study, we investigated actions metformin on experimental insulitis using STZ-induced diabetic mice.Mice with acute induced by STZ were administered gavage. Changes blood glucose and body weight, daily amount food water intake measured. Pancreatic tissues collected histologic...

Fibroblast growth factor 21 (FGF21), an endocrine predominantly secreted from liver, possesses multiple beneficial effect on energy metabolism and insulin sensitivity in animals. This study aimed to investigate the acute change of serum FGF21 response glucose challenge humans.A 75-g oral tolerance test was performed among 20 healthy subjects, 18 with impaired (IGT) type 2 diabetes mellitus (T2DM). Blood samples were collected for measurement other biochemical parameters. The associations...

Impaired wound healing is a common complication of diabetes and the leading cause lower extremity amputation. Treatment with fenofibrate, peroxisome proliferators-activated receptor α (PPARα) agonist, was associated risk amputations, particularly minor amputations without known large-vessel diseases, probably through non-lipid mechanisms. The current study aimed to test our hypothesis that fenofibrate stimulates angiogenesis restores endothelial precursor cell (EPC) function via inhibiting...

Chemotherapy is the main postsurgical and adjuvant therapy for glioma, intrinsic or acquired temozolomide (TMZ) resistance may result in poor prognosis. The miR-181 family was discovered to play an important role regulating biological functions miR-181b less expressed human gliomas as a tumor-suppressive miRNA. aim of this study explore molecular mechanism miR-181b-5p its target gene on modulating TMZ chemosensitivity glioma cells. enhanced effect cells U87MG U251 detected by MTT method....

Refractory wound is a dreaded complication of diabetes and highly correlated with EPC dysfunction caused by hyperglycemia. Acarbose widely used oral glucose-lowering drug exclusively for T2DM. Previous studies have suggested the beneficial effect acarbose on improving endothelial in patients However, no data been reported efficacy healing impairment diabetes. We herein investigated whether could improve T2DM db/db mice possible mechanisms involved. hastened enhanced angiogenesis, accompanied...

Glycyl-l-histidyl-l-lysine (GHK)-Cu is considered to be an activator of tissue remodeling, and has been used in cosmetic products. In this study, we prepared liposomes encapsulating GHK-Cu analyzed their effect on human umbilical vein endothelial cells (HUVECs) proliferation scald wound healing mice. The nanoscaled GHK-Cu-liposomes promoted HUVECs proliferation, with a 33.1% increased rate. Flow cytometry analysis showed cell number at G1 stage decreased G2 after treatment. Western blotting...

Purpose Multiple myeloma (MM) is a hematologic malignancy originating from plasma cells with clinical manifestations such as hypercalcemia, cytopenias (most commonly anemia) and renal failure. Here, we analyzed the disease burden changing trends of MM in China 1990 to 2021, aiming provide scientific effective basis for prevention control China. Methods We extracted related data Global Burden Diseases (GBD) 2021 database 2021. It described according incidence, prevalence, mortality,...

// Ying-Hui Tong 1, * , Bo Zhang 2, You-You Yan 2 Yun Fan 3 Jia-Wen Yu 1 Si-Si Kong Dan Luo Fang Su and Neng-Ming Lin Laboratory of Clinical Pharmacy, Zhejiang Cancer Hospital, Hangzhou, 310022, China Pharmacology, Translational Medicine Research Center, Hangzhou First People's Nanjing Medical University, 310006, Key Diagnosis Treatment Technology on Thoracic Oncology (Esophagus, Lung), These authors have contributed equally to this work Correspondence to: Lin, email: lnm1013@163.com...

Sepsis is a common disease that continues to increase in incidence the world. Diseases, such as diabetes mellitus, may make situation worse. Diabetic patients are at increased risk for infections. This study was designed investigate role of glibenclamide on myocardial injury by lipopolysaccharides (LPS) streptozotocin induced diabetic mice (STZ-mice).LPS used induce endotoxemia STZ-mice. Heart rate and mean arterial pressure were measured MPA-HBBS. Serum epinephrine level enzyme-linked...

Breast cancer is considered the solid tumor most sensitive to chemotherapy. However, it can become resistant various chemotherapeutic drugs, including doxorubicin, which triggers cell death by intercalation between DNA bases, free radical formation, and topoisomerase II inhibition. When drug resistance develops, several miRNAs are dysregulated, suggesting that may play a significant role in formation. In current study, we investigated how doxorubicin sensitivity of breast cells affected...

Abstract Objectives The aim of this study was to develop multifunctional fusion proteins for targeting and delivering therapy elements into glioma cells. Materials methods Multifunctional were expressed in Escherichia coli purified using Ni‐ NTA resin affinity chromatography. Human cells primary astrocytes used analyse their functions. Targeting location observed by confocal microscopy. Effects cell viability proliferation evaluated the Cell Counting Kit 8 colony formation assays. Glioma...

In this study, a polydopamine (PDA)-modified hollow fiber-immobilized xanthine oxidase (XOD) was prepared for screening potential XOD inhibitors from flavonoids. Several parameters the preparation of PDA-modified XOD, including dopamine concentration, modification time, concentration and immobilization were optimized. The results show that optimal conditions immobilized activity 2.0 mg/mL in 10.0 mM Tris-HCl buffer (pH 8.5), time 3.0 h, an 1000 μg/mL phosphate 7.5) h. Subsequently, enzymatic...

Abstract Few pieces of evidence have been published on the use Apatinib Mesylate (AM) against EGFR-TKI resistance in lung adenocarcinoma (LA) patients. Here, we investigate clinical efficacy and safety AM treatment advanced progressed epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI) resistant LA We conducted a double-blind, randomized controlled trial 68 patients admitted to 18 hospitals Anhui province China. The were evaluated terms progression-free survival (PFS),...

Background: In-depth and clear mechanistic study is a prerequisite for new drugs to enter clinical research. Method: New chemical entity BY4008 was identified by our lab as novel highly potent EGFR JAK3 dualtarget inhibitor. A cell-based test exhibited strong antiproliferative activities against SW620 HCT116 colon cancer cells harboring KRAS mutation with IC50 of nanomolar potency. Furthermore, acridine orange/ethidium bromide (AO/EB), Hematoxylin-Eosin (H&E) DAPI staining assays flow...

Oleanolic and ursolic acids were used as lead compounds to synthesize a series of pentacyclic triterpenoid derivatives bearing ethylenediamine, butanediamine, or hexanediamine groups at the C-3 position. The potential antiproliferative activity these was examined in A549 (human non-small cell lung cancer cells), MCF-7 breast HeLa cervical carcinoma cells) cells. Methyl 3β-O-[4-(2-aminoethylamino)-4-oxo-butyryl]olean-12-ene-28-oate (DABO-Me) identified promising agent vitro vivo. DABO-Me...