Mitochondria hold redox homeostasis and energy metabolism as a crucial factor during oocyte maturation, while the exposure of estrogenic mycotoxin zearalenone causes developmental incapacity in porcine oocyte. This study aimed to reveal potential resistance phytoalexin resveratrol against maturation whether its mechanism was related with PTEN-induced kinase 1 (PINK1)/Parkin-mediated mitophagy. Porcine oocytes were exposed 20 μM or without 2 vitro maturation. As for results, impaired...

The preservation of chicken embryonic cells is essential for protecting avian genetic resources and enhancing breeding programs. This study investigates the effects retinoic acid (RA) on viability, functionality, adhesion thawed blastoderm (BCs) following cryopreservation. After thawing culturing 24 h, RA treatment resulted in significantly higher cell viability rates compared to control group, with 2.0 μM group demonstrating best outcomes. 48 72 h culture, similar trends were observed,...

The conservation and utilization of livestock genetic resources is essential for the maintenance biodiversity breed innovation. Whole genome sequencing (WGS) was performed on 90 samples from Chinese Yangtze River Delta White goats (YRD), sourced two populations Chongming island white Haimen goats, aiming to dissect their genomic characteristics. In addition, 262 WGS data nine other breeds were downloaded NCBI database. These obtained used identify analyze variation with goat reference...

Mycotoxins such as zearalenone (ZEN), deoxynivalenol (DON) and T-2 toxin (T-2) are the most poisonous biological toxins in food pollution. Mycotoxin contaminations a global health issue. The aim of current study was to use porcine Leydig cells model explore toxic effects underlying mechanisms ZEN, DON T-2. 50% inhibitory concentration (IC50) ZEN 49.71 μM, IC50 values were 2.49 μM 97.18 nM, respectively. Based on IC50, exposure resulted increased cell apoptosis, well disrupted mitochondria...

In this study, we explore the effects of Poria cocos mushroom polysaccharides (PCPs) on quality and DNA methylation cryopreserved spermatozoa Shanghai white pigs. A total 24 ejaculates (three ejaculate samples per boar) from eight pigs were manually collected. The pooled semen was diluted with a based extender supplemented different concentrations PCPs (0, 300, 600, 900, 1200, 1500 μg/mL). Once thawed, their antioxidant function assessed. meantime, effect also analyzed. results show that...

In this study, we aimed to determine the benefit of mitoquinone (MitoQ) in rooster semen extenders on sperm quality, motility parameters, antioxidant capacities, and apoptotic changes post-thawed semen. A total 85 ejaculates from 18 roosters were collected then divided into five equal aliquots cryopreserved with 1.0% soy lecithin nanoparticles that contained various concentrations MitoQ (0 nM (M0), 50 (M50), 100 (M100), 150 (M150), 200 (M200)). By using a computer-assisted analyzer,...

The meat of local livestock breeds often has unique qualities and flavors. In this study, three Shanghai native pig (MSZ, SWT, SHB) exhibited better quality traits than globalized commercial (DLY). Subsequently, metabolomic lipidomic differences in the longissimus dorsi (L) gluteus (T) muscles DLY breed were compared using liquid chromatography-mass spectrometry (LC-MS). results demonstrated that metabolites mainly consisted (28.16%) lipids lipid-like molecules, (25.87%) organic acids their...

Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these on blood vessel function remain inadequately understood. We have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model, we...

Resistance to chemotherapeutic medicines complicates and eventually kills people with ovarian cancer. Nafamostat mesylate (NM) has been used as an adjuvant therapy enhance chemotherapy sensitivity in several cancers. This study aimed evaluate the effect of NM on cancer cells susceptible carboplatin (CBP) determine underlying mechanism involved. Herein, qRT-PCR, western blot, IHC were analyze mRNA protein expression. Cell viability proliferation measured using MTT colony formation assays....

Abstract Mitochondrial dysfunction is considered a crucial factor aggravating oocyte viability after vitrification‐warming. To clarify the role of mitophagy in mitochondrial extinction vitrified porcine oocytes, function, ultrastructural characteristics, mitochondria‐lysosomes colocalization, and mitophagic proteins were detected with or without chloroquine (CQ) treatment. The results showed that vitrification caused dysfunction, including increasing reactive oxygen species production,...

China has rich genetic resources of local pig breeds. In this study, whole-genome resequencing was performed on five Shanghai breeds, aiming to analyze their population structure and unique genomic characteristics. Tens millions single nucleotide variants were obtained through the a total 150 individual pigs from breeds (Meishan, Fengjing, Shawutou, Pudong White, White) after mapping them with reference genome Sus scrofa 11.1. The results admixture analysis also clearly demonstrated...

Embryo vitrification is an essential technique for <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">in vitro fertilization</i> (IVF), which avoids the formation of ice crystals during cryopreservation by dehydrating cells in cryoprotectant agents (CPAs) and ultrarapidly freezing them liquid nitrogen ( <inline-formula xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\rm LN_{2}$</tex-math></inline-formula> ). In manual...

Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these on blood vessel function remain inadequately understood. We have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model, we...

Abstract Background The transcriptome and metabolome dissection of the skeletal muscle high- low- growing individuals from a crossbred population indigenous Chongming white goat Boer were performed to discover potential functional differentially expressed genes (DEGs) differential expression metabolites (DEMs). Results A total 2812 DEGs detected in 6 groups at three time stages (3,6,12 Month) using RNA-seq method. set containing seven function related ( TNNT1 , TNNC1 TNNI1 MYBPC2 MYL2 MHY7...

Chinese indigenous pig breeds have unique genetic characteristics and a rich diversity; however, effective breed identification methods not yet been well established. In this study, genotype file of 62,822 single-nucleotide polymorphisms (SNPs), which were obtained from 1059 individuals 18 5 cosmopolitan breeds, used to screen the discriminating SNPs for identification. After linkage disequilibrium (LD) pruning filtering, study excluded 396 on non-constant chromosomes retained 20.92~-27.84%...

Vessels and nerves are closely associated in anatomy as well functions. Accumulating evidences have demonstrated that axon-guiding signals may affect endothelial cells migration path finding, which is crucial for the patterning of both complex vascular network neural system. However, studies regarding functional overlap between neuronal orchestrating still incomplete. Semaphorin6D (Sema6D) belongs to Semaphorin family has been identified an important regulating factor diverse biological...

Abstract Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these on blood vessel function remain inadequately understood. We have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model,...

Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these on blood vessel function remain inadequately understood. Presently, we have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this...

To address the safety problems posed by transportation of boar semen using LN, this study was conducted on short-term storage frozen in dry ice (−79 °C). Boar LN transferred to ice, kept for 1 day, 3 days, 5 7 or 8 and then moved back LN. The quality stored determined evaluate feasibility short-distance with ice. results showed that 60 °C s best condition thawing No significant differences spermatozoa motility, plasma membrane integrity, acrosome integrity were observed after compared (p...

This study was conducted in nutrient-restricted pregnant Hu ewes to determine whether rumen-protected arginine (RP-Arg) or N-carbamylglutamate (NCG) supplementation affects fetal liver growth and development. From 35 d 110 of gestation, 32 were randomly divided into four groups: a control group (100% the National Research Council (NRC) requirements), (50% NRC two treatment groups (ARG NCG, 50% requirements, supplemented with 20 g/day RP-Arg 5 respectively). Fetal body weights, performance,...

Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these on blood vessel function remain inadequately understood. We have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model, we...