A5100387315- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer Mechanisms and Therapy
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Gastrointestinal Tumor Research and Treatment
- Extracellular vesicles in disease
- Vascular Malformations and Hemangiomas
- Cancer Research and Treatments
- Proteoglycans and glycosaminoglycans research
- Cancer Cells and Metastasis
- Peptidase Inhibition and Analysis
- Antimicrobial Peptides and Activities
- Helicobacter pylori-related gastroenterology studies
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- Cancer, Stress, Anesthesia, and Immune Response
- Plant-Microbe Interactions and Immunity
- Glycosylation and Glycoproteins Research
- Sarcoma Diagnosis and Treatment
- Plant Virus Research Studies
Jiangsu Province Hospital
2016-2025
Nanjing Medical University
2016-2025
Guiyang Medical University
2022-2025
Guizhou University
2022-2025
Northwest A&F University
2025
Peking Union Medical College Hospital
2024-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2025
Xi’an Jiaotong-Liverpool University
2024
Lanzhou University
2022-2024
Air Force Medical University
2021-2024
Cisplatin (CDDP) treatment is one of the most predominant chemotherapeutic strategies for patients with gastric cancer (GC). A better understanding mechanisms CDDP resistance can greatly improve therapeutic efficacy in GC. Circular RNAs (circRNAs) are a class noncoding whose functions related to pathogenesis cancer, but, GC remains unknown. circAKT3 (hsa_circ_0000199, circRNA originating from exons 8, 9, 10, and 11 AKT3 gene) was identified by RNA sequencing verified quantitative reverse...
Abstract Peritoneal metastasis is a primary metastatic route for gastric cancers, and the mechanisms underlying this process are still unclear. mesothelial cells (PMCs) undergo mesothelial-to-mesenchymal transition (MMT) to provide favorable environment cancer cells. In study, we investigated how exosomal miR-21-5p induces MMT promotes peritoneal metastasis. Gastric (GC)-derived exosomes were identified by transmission electron microscopy western blot analysis, then uptake of was confirmed...
Abstract Background A novel type of noncoding RNA, circRNA has been reported to participate in the occurrence and development diseases through many mechanisms. The MAPK pathway is a common signal transduction involved cell proliferation, inflammation apoptosis plays particularly important role cancers. However, circRNAs related gastric cancer not explored. Methods bioinformatics analysis was performed profile identify cancer. tumor-suppressive circMAPK1 confirmed both vitro vivo. Mass...
An increasing number of studies indicate that adrenergic signalling plays a fundamental role in chronic stress-induced tumour progression and metastasis. However, its function gastric cancer (GC) potential mechanisms remain unknown. The expression levels β-adrenergic receptor (ADRB) GC cell lines were examined by using real-time polymerase chain reaction (RT-PCR) western blotting. effects β2 (ADRB2) activation blockade investigated vitro cells proliferation, migration, invasion, cycle...
Liver metastasis (LM) is a major obstacle to the prognosis of gastric cancer (GC) patients, but molecular mechanism underlying liver (GC-LM) remains unknown. Exosomes have been identified as an important mediator communication between tumor cells and microenvironment. Therefore, we sought investigate effects primary GC on microenvironment role exosomal microRNAs (exo-miRNA) in GC-LM.Sequential differential centrifugation, transmission electron microscopy NanoSight analysis were used extract...
Abstract Abnormal 5-methylcytosine (m5C) methylation has been proved to be closely related gastric carcinogenesis, progression, and prognosis. Dysregulated long noncoding RNAs (lncRNAs) participate in a variety of biological processes cancer. However, date, m5C-methylated lncRNAs are rarely researched cancer (GC). Here, we found that RNA cytosine-C(5)-methyltransferase (NSUN2) was upregulated GC high NSUN2 expression associated with poor NR_033928 identified as an NSUN2-methylated lncRNA GC....
Cisplatin (CDDP) resistance is a major clinical problem associated with poor prognosis in gastric cancer (GC) patients. In this study, we performed integrated analysis of TCGA data from microRNAs (miRNAs) expression matrix GC patients who received CDDP-based chemotherapy GEO dataset which contains differential miRNAs profiles CDDP-resistant and -sensitive cell lines. We identified miR-148a-3p downregulation as key step involved CDDP resistance. Using cohort consisting 105 therapy, found that...
Circular RNAs (circRNAs) play vital regulatory roles in the progression of multiple cancers. In our study, transcriptome analysis and self-organizing maps (SOM) were applied to screen backbone circRNAs gastric cancer (GC). Upon validation expression patterns screened circRNAs, gain- loss-of-function assays performed vitro vivo. Underlying mechanisms investigated using RNA pull-down, luciferase reporter assay immunoprecipitation. The circTHBS1 was significantly increased GC associated with...
Circular RNAs (circRNAs) have emerged as a new subclass of regulatory that play critical roles in various cancers. Cancer stem cells (CSCs), small subset cancer cells, are believed to possess the capacities initiate tumorigenesis and promote progression. Although accumulating evidence has suggested with CSC-like properties crucial for malignancy gastric (GC), it remains unclear whether circRNAs related acquisition GC.CircFAM73A expression was analyzed by GEO datasets verified GC samples. The...
Chemotherapy can significantly improve the disease-free survival and overall of patients with advanced gastric cancer (GC). 5-fluorouracil (5-FU) is frequently applied in clinic, acting as a first-line chemotherapy drug GC, which could be used alone or combining platinum drugs. However, its efficacy attenuated by chemoresistance, associated patients' poor survival. Recently, there evidence suggesting that dysregulation autophagy may contribute to resistance cancer, circular RNAs (circRNAs)...
Animals infected with mycoplasma pneumoniae not only develop respiratory diseases, but also cause digestive diseases through the lung-gut axis mediated by intestinal flora, and vice versa. Antimicrobial peptides are characterized their bactericidal, anti-inflammatory, flora-regulating properties. However, effect of cecropin AD (CAD) against pneumonia remains unclear. To investigate anti-inflammatory CAD on associated mechanism, mice were Mycoplasma capricolum subsp. Capripneumoniae(Mccp) to...
MicroRNAs are endogenously expressed, small non-coding RNAs that modulate gene expression by targeting specific mRNAs, resulting in translational repression or mRNA degradation. Although miR-584-5p has been reported to play a vital role various malignancies, its and the molecular mechanisms underlying effects of gastric cancer (GC) remain be clarified. In this study, we investigated GC. The target were determined human GC specimens cell lines microRNA real-time polymerase chain reaction...
Emerging evidence suggested that miRNAs can function as oncogenes or tumor suppressors by regulating downstream target genes. miR-324-3p has been reported to in several carcinomas, but its role gastric cancer (GC) is still unknown. This study aims explore the effects of on development GC. Expression was examined GC cells and tissues qRT-PCR. Effects were evaluated cell vitality assay, colony formation migration flow cytometric assay. The dual luciferase assay used verify whether could...
Abstract Increasing evidence indicates that dysregulation of microRNAs (miRNAs) plays a crucial role in human malignancies. Here, we showed microRNA-422a (miR-422a) expression was dramatically downregulated gastric cancer (GC) samples and cell lines compared with normal controls, its level inversely related to tumor size depth infiltration. Functional studies revealed the overexpression miR-422a GC cells suppressed proliferation migration, drove metabolic shift from aerobic glycolysis...
Epidemiological data show that chronic stress has adverse effects on the incidence and progression of cancer. As a critical target organ for hormones, stomach is frequently subjected to stress‑related injury. However, few reports regarding association between gastric cancer (GC) have been published. The present study aimed investigate effect growth survival GC, role autophagy process. A restraint‑stress procedure over 21 days was used establish mouse model. Subcutaneous xenografts orthotopic...
Abstract Gastric cancer remains the third leading cause of cancer-related mortality worldwide. Emerging evidence has shown that circular RNAs (circRNAs) play a critical regulatory role in occurrence and development various cancers through sponging miRNAs or acting as RNA-binding protein (RBP) sponges. We found circUBE2Q2 was significantly upregulated GC tissues cell lines. Knockdown inhibited proliferation, migration, invasion, glycolysis, increased autophagy vitro. In addition, knockdown...
Liver metastasis (LM) is one of the most common distant metastases gastric cancer (GC). However, mechanisms underlying LM GC (GC-LM) remain poorly understood. This study aimed to identify tumour-secreted protein associated with GC-LM and investigate by which this secreted remodels liver microenvironment promote GC-LM.Data-independent acquisition mass spectrometry (DIA-MS), mRNA expression microarray, quantitative real-time PCR, enzyme-linked immunosorbent assay (ELISA) immunohistochemistry...