A5100430070- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Immune cells in cancer
- IL-33, ST2, and ILC Pathways
- Circular RNAs in diseases
- Acute Kidney Injury Research
- Nanoplatforms for cancer theranostics
- Renal and related cancers
- RNA Interference and Gene Delivery
- Osteoarthritis Treatment and Mechanisms
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Nanofabrication and Lithography Techniques
- Advanced Biosensing Techniques and Applications
- COVID-19 Impact on Reproduction
- Phagocytosis and Immune Regulation
- Advanced biosensing and bioanalysis techniques
- Advanced Sensor Technologies Research
- Gastric Cancer Management and Outcomes
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Reproductive System and Pregnancy
- Scientific Measurement and Uncertainty Evaluation
- Ferroptosis and cancer prognosis
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
Nankai University
2021-2024
State Key Laboratory of Medicinal Chemical Biology
2021-2024
Lanzhou University
2020-2024
Lanzhou University Second Hospital
2024
Wannan Medical College
2021-2023
Qiqihar Medical University
2023
Tengzhou Central People's Hospital
2023
Dongguan People’s Hospital
2021-2022
Tibet Autonomous Region People's Hospital
2022
Cangzhou Central Hospital
2021
Abstract Peritoneal metastasis is a primary metastatic route for gastric cancers, and the mechanisms underlying this process are still unclear. mesothelial cells (PMCs) undergo mesothelial-to-mesenchymal transition (MMT) to provide favorable environment cancer cells. In study, we investigated how exosomal miR-21-5p induces MMT promotes peritoneal metastasis. Gastric (GC)-derived exosomes were identified by transmission electron microscopy western blot analysis, then uptake of was confirmed...
Real-time monitoring of the development atherosclerosis (AS) is key to management cardiovascular disease (CVD). However, existing laboratory approaches lack sensitivity and specificity, mostly due dearth reliable AS biomarkers. Herein, we developed an in vivo fluorescent labeling strategy that allows specific staining foam cell-derived extracellular vesicles (EVs) atherosclerotic plaques, which are released into blood as circulating biomarkers for vitro detection AS. This relies on a...
Abstract Human pluripotent stem cells (hPSCs)-derived cardiovascular progenitor (CVPCs) are a promising source for myocardial repair, while the mechanisms remain largely unknown. Extracellular vesicles (EVs) known to mediate cell–cell communication, however, efficacy and of hPSC-CVPC-secreted EVs (hCVPC-EVs) in infarct healing when given at acute phase infarction (MI) Here, we report cardioprotective effects secreted from hESC-CVPCs under normoxic (EV-N) hypoxic (EV-H) conditions infarcted...
Abstract Atherosclerosis (AS) is a chronic inflammatory disorder characterized by endothelial dysfunction. Endothelial progenitor cells (EPCs) can overcome dysfunction and reduce AS risk. This study focused on the role of EPC-secreted extracellular vesicles (EPC-EVs) in AS. First, mouse EPCs aortic (MAECs) were isolated identified. EVs from EPC-EVs co-cultured with MAECs internalization was observed. Glutathione (GSH) consumption, reactive oxygen species (ROS) production, lipid peroxidation,...
Abstract The inflammatory bowel diseases (IBD) are characterized by relapsing inflammation and immune activation of the gastrointestinal tract. Extracellular vesicles, which elicit similar biological activity to stem cell themselves, have been used experimentally treat dextran sulfate sodium (DSS)-induced colitis in murine models though immunosuppressive potential. In this study, we investigated whether vesicles (EVs) obtained stimulating cytokine on canine adipose mesenchymal cells (cASC)...
Oral squamous cell carcinoma (OSCC), accounting for two-thirds of head and neck cancer, is characterized by poor prognosis a high rate mortality. Exosomes have emerged as potential molecule-shuttle in intercellular communication, thereby regulating the physiological processes recipient cells. To date, effect exosomal microRNAs (miRNAs) on progression OSCC has not been fully investigated. In this study, we found that protein, but mRNA expression Phosphatase tensin homolog deleted chromosome...
Background: Complete abolition of alveolar epithelial cells (AECs) is characteristic end-stage lung disease.Transplantation therapy type II AECs (AEC-IIs) or AEC-IIs-derived exosomes (ADEs) have been proposed as a means repairing injury and preventing fibrosis.However, the mechanism by which ADEs balances airway immunity alleviates damage fibrosis remains unknown.Methods: We investigated STIM-activating enhancer-positive (STIMATE + ADEs) in 112 ALI/ARDS 44 IPF patients, observed correlation...
Adipose tissue derived mesenchymal stem/stromal cell (ASC)-derived extracellular vesicles (EV) have been reported to be beneficial against dextran sulfate sodium (DSS)-induced colitis in mice. However, the underlying mechanisms not fully elucidated. We hypothesize that tumor necrosis factor-α-stimulated gene/protein 6 (TSG-6) EVs is a key factor influencing alleviation of symptoms. DSS-induced mice (C57BL/6, male, Naïve = 6, Sham 8, PBS 8 EV CTL-EV TSG-6 depleted 8) were intraperitoneally...
Wounds are soft tissue injuries, which difficult to heal and can easily lead other skin diseases. Bone marrow mesenchymal stem cells (BMSCs) the secreted exosomes play a key role in wound healing. This study aims clarify effects mechanisms of derived from BMSCs Exosomes were extracted supernatant BMSCs. The expression micro-RNA miR-93-3p was determined by qRT-PCR analysis. HaCaT exposed hydrogen peroxide (H2O2) establish lesion model. MTT, flow cytometry, transwell assays conducted determine...
Acute kidney injury (AKI) is a susceptible factor for chronic disease (CKD). There still lack of effective prevention methods in clinical practice. This study investigated the protective effect urinary exosomes from premature infants on cisplatin-induced acute injury. Here we isolated fresh urine infants. A C57BL/6 mice model was given 100 ug 24 hours after establishment. The kidneys were collected pathological examination and evaluation renal tubular damage apoptosis. In vitro experiment,...
Abstract Exosomes, ranging from 30–150 nm in diameter, have emerged as promising non‐invasive biomarkers for the diagnosis and prognosis of numerous diseases. However, current research on exosomes is largely restricted by lack an efficient method to isolate real samples. Herein, first stimuli‐mediated enrichment purification system selectively efficiently extract clinical plasma high‐throughput profiling exosomal mRNAs cancer presented. This novel isolation relies specific installation...
Objective Stem-cell therapy is a promising treatment for cartilage defects. The newly identified urine-derived stem cells (USCs), which have multipotency and sufficient proliferative ability, are candidates several tissue engineering therapies. In this study, we investigated the role of USC extracellular vehicles (EVs) in promoting proliferation migration chondrocytes. Design USCs were characterized by measuring induced multipotent differentiation flow cytometry analysis surface marker...
Gastrectomy can improve the clinical survival rate of gastric cancer patients, while metabolic disorders caused by gastrectomy, seriously affect postoperative recovery. In this study, probiotics (including Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis and Bacillus cereus) were used before gastrectomy to explore effect on patients. Our outcomes revealed could alleviate inflammation (Leukocyte; P < 0.05), disorder (fasting glucose; fasting insulin; HOMA-IR; reduce...
Abstract Background The exosomes (exos) of bone marrow mesenchymal stem cells (BMSCs) play an important therapeutic role in repairing myocardial injury. purpose this study was to explore how the exos BMSCs can alleviate cell injury caused by hypoxia/reoxygenation (H/R) through HAND2-AS1/miR-17-5p/Mfn 2 pathway. Methods Cardiomyocytes H9c2 were damaged H/R mimic damage. Exos gained from BMSC. content HAND2-AS1 and miR-17-5p assessed RT-qPCR. Cell survival rate apoptosis estimated MTT assay...
Multiplexed analysis allows researchers to obtain high-density information with minimal assay time, sample volume and cost. Currently, microcarrier or particle-based approaches for multiplexed involve complicated expensive encoding decoding processes. In this paper, a novel optical technique based on nano-silicon dioxide film is presented. Microcarriers composed of thermally grown silicon (SiO(2)) monocrystalline (Si) substrate were fabricated. The exhibited unique surface color by...
Abstract Mesenchymal stem/stromal cell (MSC)-derived extracellular vesicles (EV) have been reported to be beneficial against dextran sulfate sodium (DSS)-induced colitis in mice. However, the underlying mechanisms not fully elucidated. We hypothesize that tumor necrosis factor-α-stimulated gene/protein 6 (TSG-6) EVs is a key factor influencing alleviation of symptoms. DSS-induced mice (C57BL/6, male, n = 6-8/group) were intraperitoneally administered (100 ug/mice) on day 1, 3, and 5; colon...
Exosomes are a class of membranous vesicles, which approximately 50 to 100 nm in diameter; they released into the extracellular matrix from various cells.[1] rich proteins, lipids, and nucleic acids. They have been reported be involved physiological processes important tools for intercellular material information communication.[2,3] Increasing evidence has indicated that exosomes can extracted human saliva, urine, blood, other body fluids.[4] However, whether exist nipple discharge remains...
Aim: Acute kidney injury (AKI), a global public health issue, not only causes millions of deaths every year, but is also susceptible factor for chronic disease (CKD). Nephrotoxic drugs are an important cause AKI. There still lack effective and satisfactory prevention method in clinical practice. This study investigated the protective effect exosomes derived from urine premature infants on cisplatin-induced acute injury. Methods: Isolation fresh infants: The characteristics were determined by...