A5100405715- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Neuroscience and Neuropharmacology Research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Viral Infections and Immunology Research
- EEG and Brain-Computer Interfaces
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- Chronic Lymphocytic Leukemia Research
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Neuroscience and Neural Engineering
- Monoclonal and Polyclonal Antibodies Research
Wenzhou Medical University
2014-2025
First Affiliated Hospital of Wenzhou Medical University
2014-2025
State Key Laboratory of Food Science and Technology
2025
Nanchang University
2015-2025
Air Force Medical University
2022-2025
Fudan University
2020-2025
Shanghai Ocean University
2025
Shanghai Institute of Materia Medica
2014-2025
Xinqiao Hospital
2017-2025
Army Medical University
2017-2025
Abstract Intrinsically fluorescent poly(amidoamine) dendrimers (IF‐PAMAM) are an emerging class of versatile nanoplatforms for in vitro tracking and bio‐imaging. However, limited tissue penetration their fluorescence interference due to auto‐fluorescence arising from biological tissues limit its application vivo. Herein, a green IF‐PAMAM (FGP) dendrimer is reported biocompatibility, circulation, biodistribution potential role traceable central nervous system (CNS)‐targeted delivery zebrafish...
Glycosylated “triple-interaction” stabilized siRNA nanomedicine ameliorated AD neuropathology by targeting BACE1.
Abstract Choroidal neovascularization (CNV) is an important characteristic of advanced wet age‐related macular degeneration (AMD) and leads to severe visual impairment among elderly patients. Previous studies have demonstrated that melatonin induces several biological effects related antioxidation, anti‐inflammation, anti‐angiogenesis. However, the role in CNV, its underlying mechanisms, has not been investigated thus far. In this study, we found administration significantly reduced scale...
Small interfering RNA (siRNA) has been considered as a highly promising therapeutic agent for human cancer treatment including glioblastoma (GBM), which is fatal disease without effective therapy methods. However, siRNA-based GBM seriously hampered by number of challenges in siRNA brain delivery poor stability, short blood circulation, low blood-brain barrier (BBB) penetration, and tumor accumulation, well inefficient intracellular release. Herein, an Angiopep-2 (Ang) functionalized...
Astrocytes activation in response to stroke results altered mitochondrial exchange with neurons. Ginsenoside Rb1is a major ginsenoside of Panax ginseng particularly known for its neuroprotective potential. This work aimed investigate if Rb1 could rescue neurons from ischemic insult via astrocyte inactivation and transfer. We prepared conditioned astrocytes-derived medium co-culture examined the role transfer astrocytes The potential was further confirmed vivo using mouse model brain...
Importance Tongxinluo, a traditional Chinese medicine compound, has shown promise in vitro, animal, and small human studies for myocardial infarction, but not been rigorously evaluated large randomized clinical trials. Objective To investigate whether Tongxinluo could improve outcomes patients with ST-segment elevation infarction (STEMI). Design, Setting, Participants Randomized, double-blind, placebo-controlled trial was conducted among STEMI within 24 hours of symptom onset from 124...
We have constructed a replication-deficient adenovirus encoding nonphosphorylatable Thr34→Ala mutant of the apoptosis inhibitor survivin (pAd-T34A) to target tumor cell viability in vitro and vivo. Infection with pAd-T34A caused spontaneous lines breast, cervical, prostate, lung, colorectal cancer. In contrast, did not affect proliferating normal human cells, including fibroblasts, endothelium, or smooth muscle cells. cells resulted cytochrome c release from mitochondria, cleavage...
An increased risk of premature atherosclerosis has been associated with systemic lupus erythematosus (SLE), and type I interferon (IFN) shown to play a pathogenic role in human SLE. The aim this study was determine whether IFNα is involved the development patients SLE by promoting lipid uptake macrophage-derived foam cell formation, which crucial step early atherosclerosis.The effects on formation were determined flow cytometry oil red O staining. Messenger RNA protein expression scavenger...
Short hairpin (sh)RNAs delivered by recombinant adeno-associated viruses (rAAVs) are valuable tools to study gene function in vivo and a promising therapy platform. Our data show that incorporation of shRNA transgenes into rAAV constructs reduces vector yield produces population truncated defective genomes. We demonstrate sequences with hairpins or hairpin-like structures drive the generation AAV genomes through polymerase redirection mechanism during viral genome replication. findings...
The coronavirus disease 2019 (COVID-19) pandemic has triggered a new response involving public health surveillance. popularity of personal wearable devices creates opportunity for tracking and precaution spread such infectious diseases. In this study, we propose framework, which is based on the heart rate sleep data collected from devices, to predict epidemic trend COVID-19 in different countries cities. addition physiological anomaly detection algorithm defined an online neural network...
Rationale: Optic neuritis is one of main symptoms in multiple sclerosis (MS) that causes visual disability. Astrocytes are pivotal regulators neuroinflammation MS, and astrocytic yes-associated protein (YAP) plays a critical role neuroinflammation. Meanwhile, YAP signaling involved impairment, including glaucoma, retinal choroidal atrophy detachment. However, the roles underlying mechanisms demyelination MS-related optic (MS-ON) remains unclear. Methods: To assess functions MS-ON,...
Background and Aims: The intratumoral microbiome has been reported to regulate the development progression of cancers. We aimed characterize microbial heterogeneity (IMH) establish microbiome-based molecular subtyping HBV-related HCC elucidate correlation between IMH tumorigenesis. Approach Results: A case-control study was designed investigate landscape characteristic signatures tissues adopting metagenomics next-generation sequencing. Microbiome-based established by nonmetric...
Abstract Krüppel‐like factor 2 (KLF2) belongs to the zinc finger family and is thought be a tumor suppressor gene due its low expression in various cancer types. However, functional role molecular pathway involvement colorectal (CRC) are not well defined. Herein, we investigated potential mechanism of KLF2 CRC cell invasion, migration, epithelial–mesenchymal transition (EMT). We utilized TCGA GEPIA databases analyze patients correlation with different stages prognosis. RT‐PCR, western blot,...
Aberrant tumor blood vessels are prone to propel the malignant progression of tumors, and targeting abnormal metabolism endothelial cells emerges as a promising option achieve vascular normalization antagonize progression. Herein, we demonstrated that salvianic acid A (SAA) played pivotal role in contributing tumor-bearing mice, thereby improving delivery effectiveness chemotherapeutic agent. SAA was capable inhibiting glycolysis strengthening junctions human umbilical vein (HUVECs) exposed...
The aim of this study was to establish a culture method for mouse dendritic cells (DCs) in vitro and observe their morphology at different growth stages ability induce the proliferation T lymphocytes.Granulocyte-macrophage colony stimulating factor (GM-CSF) interleukin-4 (IL-4) were used combination differentiation bone marrow (BM) mononucleocytes into DCs. derived DCs then assessed morphology, phenotype, function.The BM-derived had altered cell 3 days after induction by GM-CSF IL-4 grew...
N-methyl-D-aspartate receptors (NMDARs) mediate slow excitatory postsynaptic transmission in the central nervous system, thereby exerting a critical role neuronal development and brain function. Rare genetic variants GRIN genes encoding NMDAR subunits segregated with neurological disorders. Here, we summarize clinical presentations for 18 patients harboring 12 de novo missense GRIN1, GRIN2A, GRIN2B that alter residues M2 re-entrant loop, region lines pore is intolerant to variation. These...
Actions of adenosine triphosphate (ATP) on neurons the substantia gelatinosa (SG) were evaluated in spinal cord slices using tight-seal, whole-cell recordings. Bath-applied ATP activated a fast inward current and potentiated both glutamate-induced synaptically evoked currents by acting through purinergic receptor with pharmacology P2 type. also induced delayed slow outward depressed synaptic that appeared to result from hydrolysis adenosine. The inhibitory actions had features suggesting...