A5091825209- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Genetics and Neurodevelopmental Disorders
- Receptor Mechanisms and Signaling
- Epilepsy research and treatment
- Genomics and Rare Diseases
- RNA and protein synthesis mechanisms
- Reliability and Maintenance Optimization
- Advanced Measurement and Detection Methods
- Advanced Sensor and Control Systems
- Molecular Sensors and Ion Detection
- Probabilistic and Robust Engineering Design
- Chemical Synthesis and Analysis
- Nicotinic Acetylcholine Receptors Study
- Risk and Safety Analysis
- Amino Acid Enzymes and Metabolism
- Hydraulic and Pneumatic Systems
- Genomic variations and chromosomal abnormalities
- Advanced Computational Techniques and Applications
- Phosphodiesterase function and regulation
- Industrial Technology and Control Systems
- Image Processing Techniques and Applications
- Structural Health Monitoring Techniques
- Congenital heart defects research
- Cholinesterase and Neurodegenerative Diseases
Emory University
2016-2025
Shandong Agricultural University
2024-2025
Communication University of China
2024
XinHua Hospital
2024
China Meteorological Administration
2023
Hebei Meteorological Bureau
2023
Jilin Weather Modification Office
2023
Anyang Hospital of Traditional Chinese Medicine
2021-2023
Shanghai Institute of Technology
2022
Northwestern University
2021
Objective Early-onset epileptic encephalopathies have been associated with de novo mutations of numerous ion channel genes. We employed techniques modern translational medicine to identify a disease-causing mutation, analyze its altered behavior, and screen for therapeutic compounds treat the proband. Methods Three tools were utilized: (1) high-throughput sequencing technology novel mutation; (2) in vitro expression electrophysiology assays confirm variant protein's dysfunction; (3)...
<h3>Background</h3> We aimed for a comprehensive delineation of genetic, functional and phenotypic aspects <i>GRIN2B</i> encephalopathy explored potential prospects personalised medicine. <h3>Methods</h3> Data 48 individuals with de novo variants were collected from several diagnostic research cohorts, as well 43 patients the literature. Functional consequences response to memantine treatment investigated in vitro eventually translated into patient care. <h3>Results</h3> Overall, 86...
Adenosine triphosphate (ATP)–sensitive potassium (K ATP ) channels are activated by various metabolic stresses, including hypoxia. The substantia nigra pars reticulata (SNr), the area with highest expression of K in brain, plays a pivotal role control seizures. Mutant mice lacking Kir6.2 subunit [knockout (KO) mice] were susceptible to generalized seizures after brief In normal mice, SNr neuron activity was inactivated during hypoxia opening postsynaptic channels, whereas KO these neurons...
Astrocytes express a wide range of G-protein coupled receptors that trigger release intracellular Ca2+, including P2Y, bradykinin and protease activated (PARs). By using the highly sensitive sniffer-patch technique, we demonstrate activation P2Y receptors, all stimulate glutamate from cultured or acutely dissociated astrocytes. Of these have utilized PAR1 as model system because favourable pharmacological molecular tools, its prominent expression in astrocytes high relevance to...
NMDA receptors comprised of different NR2 subunits exhibit strikingly unique biophysical and pharmacological properties. Here, we report that the extracellular amino-terminal domain (ATD) subunit controls kinetic properties recombinant receptors, such as agonist potency, deactivation time course, open probability ( P OPEN ), mean open/shut duration. Using ATD deletion mutants NR2A, NR2B, NR2C, NR2D, chimeras NR2A NR2D with interchanged [NR2A-(2D-ATD) NR2D-(2A-ATD)], show contributes to low...
We have compared the potencies of structurally distinct channel blockers at recombinant NR1/NR2A, NR1/NR2B, NR1/NR2C and NR1/NR2D receptors. The IC 50 values varied with stereochemistry subunit composition, suggesting that it may be possible to design subunit‐selective blockers. For dizocilpine (MK‐801), differential potency MK‐801 stereoisomers determined NMDA receptors was confirmed native in vitro vivo . Since proton sensor is tightly linked both functionally gating, we examined whether...
The four <i>N</i>-methyl-d-aspartate (NMDA) receptor NR2 subunits (NR2A-D) have different developmental, anatomical, and functional profiles that allow them to serve roles in normal neuropathological situations. Identification of subunit-selective NMDA agonists, antagonists, or modulators could prove be both valuable pharmacological tools as well potential new therapeutic agents. We evaluated the potency efficacy a wide range glutamate-like compounds at NR1/NR2A, NR1/NR2B, NR1/NR2C, NR1/NR2D...
Rapid advances in sequencing technology have led to an explosive increase the number of genetic variants identified patients with neurological disease and also enabled assembly a robust database healthy individuals. A surprising GRIN genes that encode N-methyl-D-aspartate (NMDA) glutamatergic receptor subunits been found various neuropsychiatric disorders, including autism spectrum epilepsy, intellectual disability, attention-deficit/hyperactivity disorder, schizophrenia. This review...
N-methyl-D-aspartate receptors (NMDARs), ligand-gated ionotropic glutamate receptors, play key roles in normal brain development and various neurological disorders. Here we use standing variation data from the human population to assess which protein domains within NMDAR GluN1, GluN2A GluN2B subunits show strongest signal for being depleted of missense variants. We find that this includes GluN2 pre-M1 helix linker between agonist-binding domain (ABD) first transmembrane (M1). then evaluate...
Polymicrogyria is a malformation of cortical development. The aetiology polymicrogyria remains poorly understood. Using whole-exome sequencing we found de novo heterozygous missense GRIN1 mutations in 2 57 parent-offspring trios with polymicrogyria. We nine further additional patients. Shared features the patients were extensive bilateral associated severe developmental delay, postnatal microcephaly, visual impairment and intractable epilepsy. encodes GluN1, essential subunit...
NMDA receptors play crucial roles in excitatory synaptic transmission. Rare variants GRIN2A encoding the GluN2A subunit are associated with a spectrum of disorders, ranging from mild speech and language delay to intractable neurodevelopmental including but not limited developmental epileptic encephalopathy. A de novo missense variant, p.Ser644Gly, was identified child this disorder, Grin2a knock-in mice were generated model extend understanding childhood disease. Homozygous heterozygous...
This paper reviews the NTIRE 2023 challenge on efficient single-image super-resolution with a focus proposed solutions and results. The aim of this is to devise network that reduces one or several aspects such as runtime, parameters, FLOPs, activations, memory footprint, depth RFDN while at least maintaining PSNR 29.00dB DIV2K validation datasets. had 272 registered participants, 35 teams made valid submissions. They gauge state-of-the-art for super-resolution.
Abstract N-methyl- d -aspartate receptors (NMDARs) are members of the glutamate receptor family and participate in excitatory postsynaptic transmission throughout central nervous system. Genetic variants GRIN genes encoding NMDAR subunits associated with a spectrum neurological disorders. The M3 transmembrane helices couple directly to agonist-binding domains form helical bundle crossing closed that occludes pore. functions as transduction element whose conformational change couples ligand...
Phosphodiesterase 4B (PDE4B) plays a critical role in cAMP hydrolysis and is highly expressed brain regions associated with neuroinflammation central nervous system (CNS) disorders. Selective PDE4B radioligands hold significant potential for elucidating disease mechanisms, such as those Parkinson's schizophrenia, enabling target occupancy measurements. In this study, we developed [ 18 F]P4B-2412, novel PDE4B-selective radioligand, evaluated its utility positron emission tomography imaging...