A5086699090- Advanced Proteomics Techniques and Applications
- Nanoplatforms for cancer theranostics
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Analytical chemistry methods development
- Metabolomics and Mass Spectrometry Studies
- Hydrogels: synthesis, properties, applications
- Luminescence and Fluorescent Materials
- Advanced biosensing and bioanalysis techniques
- Molecular Sensors and Ion Detection
- Photodynamic Therapy Research Studies
- Electrochemical sensors and biosensors
- Polymer Surface Interaction Studies
- Analytical Chemistry and Sensors
- Glycosylation and Glycoproteins Research
- Electrochemical Analysis and Applications
- Pesticide Residue Analysis and Safety
- Advanced Nanomaterials in Catalysis
- Antimicrobial Peptides and Activities
- Advanced Sensor and Energy Harvesting Materials
- Microfluidic and Capillary Electrophoresis Applications
- Surface Modification and Superhydrophobicity
- Monoclonal and Polyclonal Antibodies Research
- Advanced Photocatalysis Techniques
- Conducting polymers and applications
Southwest Jiaotong University
2017-2025
Emory University
2025
Kaili University
2025
Hainan University
2023
Hunan Vocational Institute of Technology
2023
Dalian Institute of Chemical Physics
2003-2022
Chinese Academy of Sciences
2003-2022
Chengdu University
2019-2021
Zhejiang Institute of Freshwater Fisheries
2019
Xinjiang University
2007-2017
The mixture of phosphopeptides enriched from proteome samples are very complex. To reduce the complexity it is necessary to fractionate phosphopeptides. However, conventional enrichment methods typically only enrich but not In this study, application strong anion exchange (SAX) chromatography for and fractionation was presented. It found that were highly by SAX majority unmodified peptides did bind onto SAX. Compared with Fe(3+) immobilized metal affinity (Fe(3+)-IMAC), almost double...
Phosphorylation is one of the most important post-translational modifications proteins, which modulates a wide range biological functions and activity proteins. The analysis phosphopeptides still challenging tasks in proteomics research by mass spectrometry. In this study, novel phosphopeptide enrichment approach based on strong interaction zirconium phosphonate (ZrP) modified surface with has been developed. ZrP porous silicon (ZrP-pSi) wafer was prepared to specifically capture from...
Abstract Background: Cancers of the urinary bladder are fifth most commonly diagnosed malignancy in United States. Early clinical diagnosis cancer remains a major challenge, and development noninvasive methods for detection surveillance is desirable both patients health care providers. Approach: To identify proteins with potential utility, we enriched profiled glycoprotein component urine samples by using dual-lectin affinity chromatography liquid chromatography/tandem mass spectrometry...
Sepsis, a widely recognized disease, is characterized by multiple pathogen infections. Therefore, it imperative to develop methods that can efficiently identify and neutralize species. Phage cocktail therapy utilizes the host specificity of phages adapt infect resistant bacteria. However, its low sterilization stability efficiency lack imaging units seriously restrict application. Here, novel strategy combining aggregation-induced emission photosensitizer (AIE-PS) TBTCP-PMB with through...
Stubborn biofilm infections pose serious threats to human health due the persistence, recurrence, and dramatically magnified antibiotic resistance. Photodynamic therapy has emerged as a promising approach combat biofilm. Nevertheless, how inhibit bacterial signal transduction system efflux pump conquer recurrence resistance remains challenging unaddressed issue. Herein, boric acid-functionalized lipophilic cationic type I photosensitizer, ACR-DMP, is developed, which efficiently generates...
Developing low-toxicity, high-efficacy, and fast-acting strategies to manage acute liver injury (ALI) is critical due its rapid progression potential for severe outcomes. Curcumin (CUR) has shown promise in ALI therapy ability modulate the inflammatory microenvironment by scavenging reactive oxygen species (ROS). Nevertheless, CUR highly hydrophobic limiting bioavailability effective vivo transport, which hinders further application. In this study, we developed an microenvironment-targeted...
Phosphodiesterase 4B (PDE4B) plays a critical role in cAMP hydrolysis and is highly expressed brain regions associated with neuroinflammation central nervous system (CNS) disorders. Selective PDE4B radioligands hold significant potential for elucidating disease mechanisms, such as those Parkinson's schizophrenia, enabling target occupancy measurements. In this study, we developed [ 18 F]P4B-2412, novel PDE4B-selective radioligand, evaluated its utility positron emission tomography imaging...
Abstract With increasing drug resistance, Candida infections have posed serious threats to public health. Photodynamic therapy harnesses light destroy pathomycete, providing a smart strategy for combating of infections. However, due lack organelle targeting ability and bad extracellular polymeric substances penetrability, current photosensitizers (PSs) are far from desirable clean biofilms fight against resistance. Herein, mitochondrion aggregation‐induced emission PS, LIQ‐TPA‐TZ, was...
Phosphodiesterases (PDEs) are phosphohydrolytic enzymes responsible for degrading cyclic adenosine monophosphate (cAMP) and guanosine (cGMP), two key second messengers involved in regulating cellular functions. The PDE superfamily can be subdivided into 11 families, with PDE7 playing a crucial role the proinflammatory process, T-cell activation proliferation. As such, has emerged as potential therapeutic target treating inflammatory, immunological, neurological disorders. To date, only...
Exploring bone proteome is an important and challenging task for understanding the mechanisms of physiological/pathological process tissue. However, classical methods protein extraction soft tissues cells are not applicable Therefore, method development efficient critical analysis. We found in this study that efficiency was improved significantly when tissue demineralized by hydrochloric acid (HCl). A sequential developed large-scale analysis The first HCl solution then extracted using three...
A method for preparation of molecularly imprinted polymer (MIP) derivatized onto the surface a monolithic silica capillary column was successfully developed. The vinyl groups were first introduced monolith by immobilization gamma-methacryloxypropyltrimethoxysilane. Then MIP coating copolymerized and anchored monolith. Acetonitrile selected as porogen (solvent). other conditions, such monomer concentration, temperature, time polymerization, systematically studied. obtained MIP-derivatized...
Large-scale characterization of phosphoproteins requires highly specific methods for the purification phosphopeptides because low abundance and substoichiometry phosphorylation. A phosphopeptide enrichment method using ZrO2 nanoparticles is presented. The high specificity this approach was demonstrated by isolation from digests model phosphoproteins. strong affinity to enables a complex peptide mixture in which two orders magnitude lower than that nonphosphopeptides. Superior selectivity...
A near-infrared AIE probe was developed for super-resolution imaging and nuclear lipid droplets dynamic study with good biocompatibility high photostability.
Bacterial infections remain a major cause of morbidity worldwide due to drug resistance pathogenic bacteria. Photodynamic therapy (PDT) has emerged as promising approach overcome this resistance. However, existing photosensitizers (PSs) are broad-spectrum antibacterial agents that dysregulate the microflora balance resulting in undesirable side effects. Herein, we synthesized series aggregation-induced emission (AIE)-active PSs with lipophilic cationic AIE core varying charges, named TBTCP...
Abstract Candida albicans ( C. ), a ubiquitous polymorphic fungus in humans, causes different types of candidiasis, including oral candidiasis (OC) and vulvovaginal (VVC), which are physically mentally concerning financially costly. Thus, developing alternative antifungals that prevent drug resistance induce immunity to eliminate biofilms is crucial. Herein, novel membrane‐targeted aggregation‐induced emission (AIE) photosensitizer (PS), TBTCP‐QY, developed for highly efficient photodynamic...
There are vast archives of formalin-fixed tissues spanning many conceivable conditions such as different diseases, time courses, and treatment allowing acquisition the necessary numbers samples to carry out biomarker discovery study. However, conventional protein analysis approach is not applicable for proteins in tissue because formalin fixation process resulted cross-linking proteins, thus, intact cannot be efficiently extracted. In this study, several protocols were investigated extract...
A nanoliter trypsin-based monolithic microreactor coupled with μRPLC−MS/MS was reported for shotgun proteome analysis. The proteins were rapidly digested by the microreactor, and resulting protein digests directly loaded onto a μRPLC column separation followed detection of eluted peptides tandem mass spectrometer. digestion efficiency stability demonstrated using bovine serum albumin as model protein. When compared an incubation time more than 10 h free trypsin in conventional approach,...
Molecularly imprinted polymers (MIPs) are a kind of synthetic receptor-like materials. They have drawn more and attention in the past decades. In this work, facile method was developed to prepare porous magnetic MIPs utilizing metal coordination. The preparation is simply done using conventional oil-in-water emulsifier-free emulsion technology by mixing poly(styrene-co-itaconic acid), oxytetracyclin (OTC), Cu(II), Fe3O4 fluid one pot with reaction time 3 h. product shows high specificity...
Abstract Phagocytosis is a biological process that plays key role in host defense and tissue homeostasis. Efficient approaches for realtime imaging of phagocytosis are highly desired but limited. Herein, an AIE-active near-infrared fluorescent probe, named TBTCP , was developed fluorescence lifetime phagocytosis. could selectively label the cell plasma membrane with fast staining, wash-free process, high signal-to-background ratio, excellent photostability. Cellular statuses under different...