A5102719879- Advanced Proteomics Techniques and Applications
- Advanced Nanomaterials in Catalysis
- Advanced biosensing and bioanalysis techniques
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Mass Spectrometry Techniques and Applications
- Nanocluster Synthesis and Applications
- Electrochemical sensors and biosensors
- Electrical Fault Detection and Protection
- Medical Research and Treatments
- Enzyme Structure and Function
- Proteoglycans and glycosaminoglycans research
- Bladder and Urothelial Cancer Treatments
- High voltage insulation and dielectric phenomena
- Ginseng Biological Effects and Applications
- Carbon and Quantum Dots Applications
- Microtubule and mitosis dynamics
- Galectins and Cancer Biology
- Protein Structure and Dynamics
- Heat shock proteins research
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Porphyrin and Phthalocyanine Chemistry
- FOXO transcription factor regulation
- Advanced Biosensing Techniques and Applications
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
Xiyuan Hospital
2021
Jilin University
2013-2021
Jilin Medical University
2020
The University of Texas MD Anderson Cancer Center
1994-2020
Thermo Fisher Scientific (United States)
2019
Central South University
2013-2018
Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération
2012
Beijing Normal University
2012
University of Michigan
2010-2011
Abstract Background: Cancers of the urinary bladder are fifth most commonly diagnosed malignancy in United States. Early clinical diagnosis cancer remains a major challenge, and development noninvasive methods for detection surveillance is desirable both patients health care providers. Approach: To identify proteins with potential utility, we enriched profiled glycoprotein component urine samples by using dual-lectin affinity chromatography liquid chromatography/tandem mass spectrometry...
In order to discover potential glycoprotein biomarkers in ovarian cancer, we applied a lectin array and Exactag labeling based quantitative glycoproteomics approach. A strategy was used detect overall lectin-specific glycosylation changes serum proteins from patients with cancer those benign conditions. Lectins, which showed significant differential response for fucosylation, were extract glycoproteins that had been labeled using isobaric chemical tags. The then identified quantified by...
Herein, we designed a new strategy for fabricating renewable bioresource-derived N-doped hierarchical porous carbon-supported iron (Fe/NPC)-based oxidase mimic. The obtained results suggested that Fe/NPC possessed large specific surface area (1144 m2/g) and pore volume (0.62 cm3/g) to afford extensive Fe-Nx active sites. Taking advantages of the remarkable oxidase-mimicking activity, outstanding stability, reusability Fe/NPC, novel dual-channel biosensing system was strategically fabricated...
A mass spectrometric method was developed to elucidate the N-glycan structures of serum glycoproteins and utilize fucosylated glycans as potential markers for pancreatic cancer. This assay applied haptoglobin in human where N-glycans derived from 16 cancer patients were compared with those 15 individuals benign conditions (5 normals, 5 chronic pancreatitis, type II diabetes). used only 10 μL extracted using a monoclonal antibody quantitative permethylation performed on desialylated followed...
Despite progress in the treatment of glioblastoma, more than 95% patients suffering from this disease still die within 2 years. Recent findings support belief that cancer stem-like cells are responsible for tumor formation and ongoing growth. Here a method combining lectin microarray LC-MS/MS was used to discover cell surface glycoprotein markers glioblastoma-derived line. Lectin analysis glycans showed two galactose-specific lectins Trichosanthes kirilowii agglutinin (TKA) Peanut (PNA)...
Mitoribosome in mammalian cells is responsible for synthesis of 13 mtDNA-encoded proteins, which are integral parts four mitochondrial respiratory chain complexes (I, III, IV and V). ERAL1 a nuclear-encoded GTPase important the formation 28S small mitoribosomal subunit. Here, we demonstrate that knockdown by RNA interference inhibits protein promotes reactive oxygen species (ROS) generation, leading to autophagic vacuolization HeLa cells. Cells lack expression showed significant conversion...
A colorimetric strategy for the detection of ALP activity based on Fe/NC-SAs.
Background: Previous research suggested that Chinese Medicine (CM) Formula Huashibaidu granule might shorten the disease course in coronavirus 2019 (COVID-19) patients. This aimed to investigate early treatment effect of well-managed patients with mild COVID-19. Methods: An unblinded cluster-randomized clinical trial was conducted at Dongxihu FangCang hospital. Two cabins were randomly allocated a CM or control group, 204 COVID-19 participants each cabin. All received conventional over 7 day...
Cancer stem cells are responsible for tumor formation through self-renewal and differentiation into multiple cell types thus represent a new therapeutic target tumors. Glycoproteins play critical role in determining the fates of such as self-renewal, proliferation, differentiation. Here we applied multilectin affinity chromatography quantitative glycoproteomics approach to analyze alterations glycoproteins relevant glioblastoma-derived line HSR-GBM1. Three lectins including concanavalin A...
Cisplatin (cis-Pt) resistance in tumor cells from p53 dysfunction is a significant clinical problem. Although mutation can inhibit function, >60% of mutants retain normal function according to literature reports. Therefore, we examined the status cisplatin-resistant ovarian models and its functional response cis-Pt mechanistically-distinct non-cross-resistant oxaliplatin (oxali-Pt). Relative sensitive A2780 harboring wild-type p53, 2780CP/Cl-16, OVCAR-10, Hey OVCA-433 cell lines were 10-...
Alterations in cellular phosphorylation patterns have been implicated a number of diseases, including cancer, through multiple mechanisms. Herein we present survey the profiles an isogenic pair human cancer cell lines with opposite metastatic phenotype. Phosphopeptides were enriched from tumor lysates titanium dioxide and zirconium dioxide, identified nano-LC-MS/MS using automatic cross-validation MS/MS MS/MS/MS (MS2+MS3) data-dependent neutral loss method. A spectral counting quantitative...
The aim of this study was to investigate the shared molecular pathways obesity and cancer by exploring role RIZ1 in phospatidylinositol 3-kinase (PI3K)/V-Akt murine thymoma viral oncogene homolog (PKB) (AKT)/mechanistic target rapamycin (mTOR) pathway.Male wild type (WT) Riz1(-/-) mice (KO) were fed a standard diet (STD) or high-fat (HF) for up 8 months. These studied phenotypic changes.Riz1(-/-) gained more weight on HF compared WT mice, with higher free fatty acid increased visceral fat....
RIZ1 has been studied as a tumor suppressor and may play role in metabolic diseases related to the Western style diet, such cancer obesity. The Akt pathway is known both obesity, link between also found. To better understand of obesity cancer, we investigated how regulates expression Akt3. We found that overexpression HEK293 cells reduced Akt3 protein. Luciferase reporter activity gene promoter was significantly co-transfected with RIZ1. Recombinant proteins able bind vitro, chromatin...
The rapid and accurate monitoring of viral genes plays an important role in the area disease diagnosis, biomedical research, food safety. Herein, we successfully designed a sensing system that combined technologies target DNA recycling amplification, magnetic separation, situ formation fluorescent copper nanoclusters (CuNCs) for analysis. In presence (tDNA), large quantity output (oDNA) was produced from hairpin (hDNA) through exonuclease III-assisted amplification strategy. Magnetic beads...
Partial discharge (PD) detection is considered as a reliable method to analyze the operating status of electrical equipment. But there are only few literatures about PDs cross-linked polyethylene (XLPE) cable at DC voltage. So this paper mainly concentrates on electric field distribution and PD phenomena XLPE cable. Numerical simulation applied in normal four typical insulation defect (corona defect, cavity scratch creepage defect) models. Electro-thermal coupling model established solve...
Dysfunction of p53 and resistance to cancer drugs can arise through mutually exclusive overexpression MDM2 or MDM4. Cisplatin-resistant cells, however, demonstrate increased binding both MDM4 but in absence cellular overexpression. Whether inhibitors alone activate these resistant cells was investigated with the goal establish mechanism for potential synergy cisplatin. Thus, growth inhibition by individual combinations assessed a colorimetric assay. Drug-treated parental A2780 tumor were...