The production of paper-based bacterial cellulose–chitosan (BC–Ch) nanocomposites was accomplished following two different approaches. In the first, BC paper sheets were produced and then immersed in an aqueous solution chitosan (BC–ChI); second, pulp impregnated with prior to (BC–ChM). BC–Ch investigated terms physical characteristics, antimicrobial antioxidant properties, ability inhibit formation biofilms on their surface. types maintained hydrophobic character, air barrier high...

Xylanase A from the recently isolated Bacillus sp. strain BP-23 was purified to homogeneity. The enzyme shows a molecular mass of 32 kDa and an isoelectric point 9.3. Optimum temperature pH for xylanase activity were 50 degrees C 5.5 respectively. completely inhibited by N-bromosuccinimide. main products birchwood xylan hydrolysis xylotetraose xylobiose. shown facilitate chemical bleaching pulp, generating savings 38% in terms chlorine dioxide consumption. amino-terminal sequence has...

Purified walls from Saccharomyces cerevisiae were treated chemically to release intrinsic mannoproteins. Boiling in 2% SDS gave the best results, although treatment 6 M-urea at room temperature also released significant amounts of mannoprotein radioactivity. Triton X-100, sodium deoxycholate and EDTA poor solubilizers. Electrophoretic patterns SDS- or urea-released mannoproteins SDS-acrylamide gels indicated a great heterogeneity molecular species, with more than 60 bands. Zymolyase,...

Xyn30D from the xylanolytic strain Paenibacillus barcinonensis has been identified and characterized. The enzyme shows a modular structure comprising catalytic module family 30 (GH30) carbohydrate-binding 35 (CBM35). Like GH30 xylanases, recombinant efficiently hydrolyzed glucuronoxylans methyl-glucuronic acid branched xylooligosaccharides but showed no activity on arabinose-substituted xylans. Kinetic parameters of were determined beechwood xylan, showing K(m) 14.72 mg/ml k(cat) value 1,510...

A Gram-positive, endospore-forming, xylanase-producing bacterium isolated from a rice field was studied taxonomically. The strain grows at 10–40 °C and in the presence of lysozyme or 5 % (w/v) NaCl. Chemotaxonomic analysis revealed that MK-7 predominant menaquinone strain, while major fatty acid anteiso-C 15 : 0 . Comparison 16S rRNA gene sequences showed BP-23 T fell within radiation cluster comprising Paenibacillus species. highest sequence similarities were found with illinoisensis (97·4...

Aims: To isolate new fungal strains from subtropical soils and to identify those that produce high cellulase activity. select microbial producing thermostable cellulases with potential application in industry. Methods Results: The Penicillium sp. CR-316 CR-313 have been identified selected because they secreted a level of media supplemented rice straw. Analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis, isoelectric focussing zymography showed the studied multiple enzymes...

A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference other GH5 xylanases characterized date that show requirement for methyl-glucuronic acid side chains catalysis. enzyme evaluated Eucalyptus kraft pulp, its effectiveness as bleaching aid.

Enzymatic biodiesel synthesis can solve several problems posed by the alkaline-catalyzed transesterification but it has drawback of being too expensive to be considered competitive. Costs reduced lipase improvement, use unrefined oils, evaluation soluble/immobilized preparations, and combination phospholipases with a soluble for production in single step. As shown here, convenient natural tools have been developed that allow high quality FAMEs (EN14214) from oils completely enzymatic...

The increasing interest in replacing petroleum-based products by more sustainable materials the packaging sector gives relevance to cellulose as a biodegradable natural resource. Moreover, its properties can be modified physically, chemically or biotechnologically order obtain new bioproducts. Refined cotton linters with high content were treated hydrolytic (cellulases) and oxidative (LPMO Laccase_Tempo) enzymes evaluate their effect on fibre improving mechanical fibrillation.Cellulases...

The gene xynC encoding xylanase C from Bacillus sp. BP-23 was cloned and expressed in Escherichia coli. nucleotide sequence of a 3538 bp DNA fragment containing determined, revealing an open reading frame 3258 that encodes protein 120567 Da. A comparison the deduced amino acid with known β-glycanase sequences showed encoded enzyme is modular three different domains. central region catalytic domain, which shows high homology to family 10 xylanases. domain homologous IX cellulose-binding...

The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase isolated cloned in Escherichia coli. enzyme displayed high activity on short to medium chain length (C4–C8) substrates, poor C18 substrates. On basis amino acid sequence homology, classified into subfamily I.4 bacterial lipases.

The gene yvpA from Bacillus subtilis was cloned and expressed in Escherichia coli . It encoded a pectate lyase of 221 amino acids that denominated PelC. heterologously enzyme purified by His-tag affinity chromatography characterized. PelC depolymerized polygalacturonate pectins methyl esterification degree 22 % to 89 %, exhibiting maximum activity on esterified citrus pectin. showed an absolute Ca 2+ requirement the optimum temperature pH were 65 °C 10, respectively. deduced acid sequence 53...

Paenibacillus barcinonensis is a soil bacterium bearing complex set of enzymes for xylan degradation, including several secreted and Xyn10B, one the few intracellular xylanases reported to date. The crystal structure Xyn10B has been determined by x-ray analysis. enzyme folds into typical (β/α)8 barrel family 10 glycosyl hydrolases (GH10), with additional secondary elements within β/α motifs. One these loops -L7- located at β7 C terminus, was essential xylanase activity as its partial...

Xylanase A from Paenibacillus barcinonensis, a new species isolated rice field, has been cloned and expressed in Escherichia coli. Purified recombinant xylanase showed high activity on xylans hardwoods cereals, exhibited Km Vmax of 2.93 mg/mL 50.67 U/mg birchwood xylan. was highly active at 60 °C alkaline pH values up to 9.5 remained stable for least 3 h conditions. The amino acid sequence deduced xynA revealed that it is single domain belonging the GH10 family. Thin layer chromatography...

Bacterial lipases constitute the most important group of biocatalysts for synthetic organic chemistry. Accordingly, there is substantial interest in developing new valuable lipases. Considering lack information concerning genus Rhodococcus and taking into account raised by enzymes produced actinomycetes, a search putative lipase-encoding genes from sp. strain CR-53 was performed. We isolated, cloned, purified, characterized LipR, first lipase described Rhodococcus. LipR mesophilic enzyme...