A5101917353- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Urinary Bladder and Prostate Research
- Computational Drug Discovery Methods
- Coronary Interventions and Diagnostics
- Retinal and Macular Surgery
- Glaucoma and retinal disorders
- Cell Image Analysis Techniques
- Circadian rhythm and melatonin
- Memory and Neural Mechanisms
- Urological Disorders and Treatments
- Neuroscience and Neural Engineering
- Pelvic floor disorders treatments
- Biomedical Text Mining and Ontologies
- Advanced Memory and Neural Computing
- Protein Structure and Dynamics
- Cerebrovascular and Carotid Artery Diseases
- Retinal Imaging and Analysis
- Neuroscience and Music Perception
- SARS-CoV-2 and COVID-19 Research
- Sleep and Wakefulness Research
Army Medical University
2017-2024
Guangxi University
2024
Suzhou Institute of Biomedical Engineering and Technology
2024
Chinese Academy of Sciences
2024
Otto-von-Guericke University Magdeburg
2024
Leibniz Institute for Neurobiology
2024
Center for Behavioral Brain Sciences
2024
South China University of Technology
2024
Army Medical College
2024
Shandong Normal University
2021-2023
Abstract Neurostimulant drugs or magnetic/electrical stimulation techniques can overcome attention deficits, but these are weakly beneficial in boosting the learning capabilities of healthy subjects. Here, we report a technique, mid-infrared modulation (MIM), that delivers light energy through opened skull even non-invasively thinned intact and activate brain neurons vivo without introducing any exogeneous gene. Using c-Fos immunohistochemistry, single-cell electrophysiology two-photon Ca 2+...
Abstract Motivation: Identifying drug–drug interactions (DDIs) is a critical process in drug administration and development. Clinical support tools often provide comprehensive lists of DDIs, but they usually lack the supporting scientific evidences different can return inconsistent results. In this article, we propose novel approach that integrates text mining automated reasoning to derive DDIs. Through extraction various facts metabolism, not only DDIs are explicitly mentioned be extracted...
Abstract Lipid deposition inside the arterial wall is a key indicator of plaque vulnerability. An intravascular photoacoustic (IVPA) catheter considered promising device for quantifying amount lipid wall. Thus far, IVPA systems suffered from slow imaging speed (~50 s per frame) due to lack suitable laser source high-speed excitation molecular overtone vibrations. Here, we report an improvement in by two orders magnitude, 1.0 frame, enabled custom-built, 2-kHz master oscillator power...
Automatic sleep stage classification plays an essential role in quality measurement and disorder diagnosis. Although many approaches have been developed, most use only single-channel electroencephalogram signals for classification. Polysomnography (PSG) provides multiple channels of signal recording, enabling the appropriate method to extract integrate information from different achieve higher staging performance. We present a transformer encoder-based model, MultiChannelSleepNet, automatic...
Abstract The sensory responses of cortical neuronal populations following training have been extensively studied. However, the spike firing properties individual neurons remain unknown. Here, we combined two-photon Ca 2+ imaging and single-cell electrophysiology in awake behaving mice auditory associative training. We find a sparse set (~5%) layer 2/3 primary cortex, each which reliably exhibits high-rate prolonged burst to trained sound. Such bursts are largely absent cortex untrained mice....
For the diagnosis of atherosclerosis, biomedical imaging techniques such as intravascular ultrasound (IVUS) and optical coherence tomography (OCT) have been developed. The combined use IVUS OCT is hypothesized to remarkably increase diagnostic accuracy vulnerable plaques. We developed an integrated IVUS-OCT apparatus, which includes catheter, motor drive unit, system. dual-function catheter has same diameter current clinical standard. system capable for simultaneous in real time. Ex vivo...
We developed a multimodality fluorescence and optical coherence tomography probe based on double-clad fiber (DCF) combiner. The is composed of DCF combiner, grin lens, micromotor in the distal end. An integrated swept-source intensity imaging system was combined for early diagnoses atherosclerosis. This capable real-time data acquisition processing as well image display. For imaging, inflammation atherosclerosis necrotic core formed with annexin V-conjugated Cy5.5 were imaged. Ex vivo New...
The outbreak caused by SARS-CoV-2 has received extensive worldwide attention. As the main protease (Mpro) in no human homologues, it is feasible to reduce possibility of targeting host protein accidental drugs. Thus, Mpro been an attractive target efficient drug design for anti-SARS-CoV-2 treatment. In this work, multiple replica molecular dynamics (MRMD) simulations, principal component analysis (PCA), free energy landscapes (FELs), and mechanics-generalized Born surface area (MM-GBSA)...
The pneumonia outbreak caused by the SARS-CoV-2 virus poses a serious threat to human health and world economy. development of safe highly effective antiviral drugs is great significance for treatment COVID-19. main protease (Mpro) key enzyme viral replication transcription has no homolog in humans. Therefore, Mpro an ideal target design against Insights into inhibitor-Mpro binding mechanism conformational changes are essential potent that Mpro. In this study, we analyzed induced three...
Monitoring astrocytic Ca 2+ activity is essential to understand the physiological and pathological roles of astrocytes in brain. However, previous commonly used methods for studying activities can be applied only anesthetized or head-fixed animals, which significantly affects vivo dynamics. In current study, we combined optic fiber recordings with genetically encoded indicators (GECIs) monitor freely behaving mice. This approach enabled selective reliable measurement activity, was verified...
In this Letter, we present a trimodality imaging system and an intravascular endoscopic probe for the detection of early-stage atherosclerotic plaques. The integrated is able to acquire optical coherence tomography (OCT), fluorescence, ultrasound images simultaneously display them in real time. A 1.2 mm diameter 7 length was fabricated based on dual-modality that integrates OCT fluorescence functions miniature transducer. capable rotating at up 600 rpm. Ex vivo from rabbit aorta human...
A pilot study was carried out to determine individual differences in perceived loneliness using diffusion tensor imaging. To the best of our knowledge, this is first preliminary imaging evidence that ventral attention network, generally activated by attentional reorienting, also related loneliness. Image reconstruction results indicated significantly decreased fractional anisotropy white matter fibers and associated nodes network are highly correlated with increased ratings. By providing on...
Cyclin-dependent kinase 2 (CDK2) has been regarded as a promising drug target for anti-tumour agents. In this study, molecular dynamics (MD) simulations and principal component (PC) analysis were used to explore binding mechanism of three inhibitors 1PU, CDK, 50Z CDK2 influences their bindings on conformational changes CDK2. The results show that yield obvious impacts internal dynamics, movement patterns addition, mechanics generalized Born surface area (MM-GBSA) was applied calculate free...
The ventrolateral periaqueductal gray (VLPAG) is thought to be the main PAG column for bladder control. neurons (especially VLPAG neurons) and in pontine micturition center (PMC) innervating detrusor have anatomical functional synaptic connections. prevailing viewpoint on neural control of that receive information decision void made by upstream brain regions, consequently activate PMC through their direct projections initiate urination reflex. However, exact location PMC-projecting neurons,...
Fungal keratitis (FK) is a common and severe corneal disease, which widely spread in tropical subtropical areas. Early diagnosis treatment are vital for patients, with confocal microscopy cornea imaging being one of the most effective methods FK. However, cases currently diagnosed by subjective judgment ophthalmologists, time-consuming heavily depends on experience ophthalmologists. In this paper, we introduce novel structure-aware automatic algorithm based deep convolutional neural networks...
Locomotion involves complex neural activity throughout different cortical and subcortical networks. The primary motor cortex (M1) receives a variety of projections from brain regions is responsible for executing movements. visual (V1) external stimuli plays an important role in guiding locomotion. Understanding how exactly the M1 V1 are involved locomotion requires recording activities these areas freely moving animals. Here, we used optical fiber-based method real-time monitoring neuronal...
In vivo two-photon Ca2+ imaging is a powerful tool for recording neuronal activities during perceptual tasks and has been increasingly applied to behaving animals acute or chronic experiments. However, the auditory cortex not easily accessible because of abundant temporal muscles, arteries around ears their lateral locations. Here, we report protocol in head-fixed mice. By using custom-made head fixation apparatus head-rotated procedure, achieved combination with targeted cell-attached...