A5100603551- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Ocular Infections and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Optical Coherence Tomography Applications
- Retinal and Optic Conditions
- Intraocular Surgery and Lenses
- Connexins and lens biology
- Allergic Rhinitis and Sensitization
- RNA Interference and Gene Delivery
- Ophthalmology and Visual Impairment Studies
- Immune Response and Inflammation
- Advanced Glycation End Products research
- Dermatologic Treatments and Research
- Advanced Drug Delivery Systems
- Image Processing Techniques and Applications
- Ocular Disorders and Treatments
- Visual perception and processing mechanisms
- Retinal and Macular Surgery
- Inflammasome and immune disorders
- Heme Oxygenase-1 and Carbon Monoxide
Sun Yat-sen University
2016-2025
Beijing Tongren Hospital
2025
Capital Medical University
2025
Puer University
2025
Yichang Central People's Hospital
2025
Boston University
2025
Shandong Agricultural University
2024
State Key Laboratory of Ophthalmology
2009-2023
Jiangsu Hengrui Medicine (China)
2023
Shunyi Hospital of Beijing Traditional Chinese Medicine Hospital
2023
Fungal keratitis, a severe ocular disease, is one of the leading causes morbidity and blindness, yet it often neglected, especially in developing countries. Therapeutic efficacy traditional treatment such as eye drops very limited due to poor bioavailability, whereas intraocular injection might cause serious side effects. Herein, we designed fabricated hybrid hydrogel-based contact lens which comprises quaternized chitosan (HTCC), silver nanoparticles, graphene oxide (GO) with combination...
Corneal transplantation is the most effective clinical treatment for corneal defects, but it requires precise size of donor corneas, surgical sutures, and overcoming other technical challenges. Postoperative patients may suffer graft rejection complications caused by sutures. Ophthalmic glues that can long-term integrate with tissue effectively repair focal damage are highly desirable. Herein, a hybrid hydrogel consisting porcine decellularized stroma matrix (pDCSM) methacrylated hyaluronic...
Corneal alkali burns represent a prevalent ophthalmic emergency with the potential to induce blindness. The main contributing mechanisms include excessive inflammation and delayed wound healing. Existing clinical therapies have limitations, promoting exploration of alternative methods that offer improved efficacy reduced side effects. Adipose-derived stem cell-exosome (ADSC-Exo) has sustain immune homeostasis facilitate tissue regeneration. Nevertheless, natural ADSC-Exo lacks disease...
Keratoplasty is the gold standard treatment for visual impairment caused by corneal damage. The use of suturing as bonding method source many complications following keratoplasty. Currently available adhesives do not have both adequate adhesive strength and acceptable biocompatibility. Herein, we developed a photocurable bioadhesive hydrogel which was composed gelatin methacryloyl oxidized dextran sutureless exhibited high light transmittance, resistance to enzymatic degradation excellent It...
To evaluate the precise mode of cell death and to investigate molecular mechanism underlying initiation inflammation in dry eye disease (DED).C57BL/6 mice were injected with scopolamine subcutaneously exposed desiccating stress establish a DED mouse model. An immortalized human corneal epithelial line (HCEC) was cultured under hyperosmolarity (500 mOsM). Protein expressions measured using western blot assay immunofluorescence staining. mRNA expression analyzed by RNA-sequencing quantitative...
The damage of corneal epithelium may lead to the formation irreversible opacities and even blindness. migration rate epithelial cells directly affects repair. Here, we explored ocu-microRNA 24-3p (miRNA 24-3p) that can promote rabbit cornea Exosomes, an excellent transport carrier, were exacted from adipose derived mesenchymal stem for loading with miRNA prepare 24-3p-rich exosomes (Exos-miRNA 24-3p). It accelerate in vitro vivo. For application alkali burns, further modified hyaluronic acid...
Nuclear cataract (NC) is a leading eye disease for blindness and vision impairment globally. Accurate objective NC grading/classification essential clinically early intervention surgery planning. Anterior segment optical coherence tomography (AS-OCT) images are capable of capturing the nucleus region clearly measuring opacity quantitatively. Recently, clinical research has suggested that correlation repeatability between severity levels average density on AS-OCT high with interclass...
Abstract Blindness caused by corneal damage affects millions of people worldwide, and this number continues to rise. However, rapid epithelization a stable epithelium process are the two biggest challenges for traditional materials. These processes related curvature, which is an important factor in determination healing epithelial behavior during damage. In study, smooth 3D‐printed convex implants based on gelatin methacrylate collagen generated. As distribution adhesion vary different...
Purpose: The purpose of this study was to elucidate the involvement potassium two pore domain channel subfamily K member 5 (KCNK5)-mediated efflux in pathogenesis dry eye and unravel underlying molecular mechanisms. Methods: To induce experimental adult wild-type C57BL/6 mice, scopolamine administered via subcutaneous injection, mice were subjected desiccating stress. create an vitro model eye, desiccation stress applied human corneal epithelial cell line (HCE-T). Intracellular concentration...
Abstract Meibomian gland dysfunction (MGD) is a leading cause of evaporative dry eye disease, presenting challenge for targeted treatment. Traditional topical ocular drug delivery methods often fail to effectively reach the meibomian glands (MGs). To address this, study has developed soluble microneedles (MN) patch comprising poly(vinyl alcohol), cyclodextrin modified polyacrylic acid, and new indocyanine green. This innovative MN facilitates transdermal release peroxisome...
Abstract Adult stem cell identity, plasticity, and homeostasis are precisely orchestrated by lineage-restricted epigenetic transcriptional regulatory networks. Here, integrating super-enhancer chromatin accessibility landscapes, we delineate core transcription circuitries (CRCs) of limbal stem/progenitor cells (LSCs) find that RUNX1 SMAD3 required for maintenance corneal epithelial identity homeostasis. or depletion inhibits PAX6 induces LSCs to differentiate into epidermal-like cells....
This study aimed to investigate the protective effect of melatonin on corneal epithelium in dry eye disease(DED) and explore its underlying mechanism. Human epithelial(HCE) cells was exposure t-butylhydroperoxide(tBH), C57BL/6 mice were injected subcutaneous scopolamine imitate DED. Melatonin used both vivo vitro. Cell viability detected by Counting Kit-8 assay Lactate Dehydrogenase Leakage. The change cellular reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP),...
Abstract Penetrating corneal wounds can cause severe vision impairment and require prompt intervention to restore globe integrity minimize the risk of infection. Tissue adhesives have emerged as a promising alternative suturing for mitigating postoperative complications. However, conventional water‐soluble suffer formidable challenges in sealing penetrating due dilution or loss moist environment. Inspired by robust adhesion mussels aquatic conditions, an injectable photocurable bioadhesive...
ABSTRACT As a novel family of cell surface receptors, triggering receptors expressed on myeloid cells (TREMs) play an important role in inflammatory responses. However, the TREMs ocular immune system remains unknown. In this study, we examined expression and function TREM-1 Pseudomonas aeruginosa keratitis, one most common sight-threatening diseases. was significantly increased human corneas after P. infection. Consistent with at surface, levels (mRNA protein) were also elevated infected...
PAX6 is a master regulatory gene involved in neuronal cell fate specification. It also plays critical role early eye field and subsequent limbal stem (LSC) determination during development. Defects Pax6 cause aniridia LSC deficiency humans the Sey (Small eye) phenotype mice (Massé, K., Bhamra, S., Eason, R., Dale, N., Jones, E. A. (2007) Nature 449, 1058-1062). However, how specifies corneal fates development not well understood. Here, we show that expressed primitive cup later tissue...
miR-155 (microRNA-155) is an important noncoding RNA in regulating host inflammatory responses. However, its regulatory role ocular infection remains unclear. Our study first explored the function of Pseudomonas aeruginosa-induced keratitis, one most common sight-threatening diseases. We found that expression was enhanced human and mouse corneas after P. aeruginosa mainly expressed macrophages but not neutrophils. In vivo studies demonstrated knockout mice displayed more resistance to with a...
To assess how DNA damage-inducible transcript 4 (DDIT4) and autophagic flux are altered in dry eye disease reveal the underlying mechanisms.C57BL/6 mice were exposed to desiccating stress (subcutaneous scopolamine [0.5 mg/0.2 mL] 3 times a day, humidity < 30%) for 7 days. Primary human corneal epithelial cells from cell line cultured under hyperosmolarity. Western blot assays immunofluorescence staining used measure changes protein expression. mRNA expression was analyzed by RT-PCR...