A5100742025- Single-cell and spatial transcriptomics
- Liver Disease Diagnosis and Treatment
- Gut microbiota and health
- Hepatitis B Virus Studies
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Gene expression and cancer classification
- Gene Regulatory Network Analysis
- Bioinformatics and Genomic Networks
- Cell Image Analysis Techniques
- Epigenetics and DNA Methylation
- Medical Imaging and Pathology Studies
- Lung Cancer Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- Global Health Care Issues
- Photosynthetic Processes and Mechanisms
- Immune Response and Inflammation
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Thermochemical Biomass Conversion Processes
- Opportunistic and Delay-Tolerant Networks
- Chromosomal and Genetic Variations
- Coal and Coke Industries Research
- Aquaculture disease management and microbiota
Peking Union Medical College Hospital
2023-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2025
Jining Medical University
2024
Tsinghua University
2021-2024
Ministry of Education of the People's Republic of China
2024
Northwest University
2024
Qingdao University of Science and Technology
2024
Northeast Health
2024
Beibu Gulf University
2023
University of Pittsburgh
2023
Significance We provide a method for population-based structure modeling of whole diploid genomes using Hi-C data. The considers the stochastic nature chromosome structures, which allows detailed analysis dynamic landscape genome organizations. predict and experimentally validate presence chromosome-specific higher-order centromere clusters, can play key role in spatial organization human genome, specifically influencing overall positioning, as well preference specific conformations. Our...
Abstract Blindness caused by corneal damage affects millions of people worldwide, and this number continues to rise. However, rapid epithelization a stable epithelium process are the two biggest challenges for traditional materials. These processes related curvature, which is an important factor in determination healing epithelial behavior during damage. In study, smooth 3D‐printed convex implants based on gelatin methacrylate collagen generated. As distribution adhesion vary different...
Regulatory T (T reg ) cells and cancer-associated fibroblasts (CAFs) jointly promote tumor immune tolerance tumorigenesis. The molecular apparatus that drives cell CAF coordination in the microenvironment (TME) remains elusive. Interleukin 33 (IL-33) has been shown to enhance fibrosis IL1RL1 + accumulation during tumorigenesis tissue repair. We demonstrated signaling greatly dampened antitumor activity of both IL-33 PD-1 blockade. Whole single-cell RNA sequencing (scRNA-seq) analysis...
Cell-cell communication events (CEs) are mediated by multiple ligand-receptor (LR) pairs. Usually only a particular subset of CEs directly works for specific downstream response in microenvironment. We name them as functional (FCEs) the target responses. Decoding FCE-target gene relations is: important understanding mechanisms many biological processes, but has been intractable due to mixing factors and lack direct observations. developed method HoloNet decoding FCEs using spatial...
The accumulation of massive single-cell omics data provides growing resources for building biomolecular atlases all cells human organs or the whole body. true assembly a cell atlas should be cell-centric rather than file-centric. We developed unified informatics framework seamless and built Ensemble Cell Atlas (hECA) from scattered data. hECA v1.0 assembled 1,093,299 labeled 116 published datasets, covering 38 11 systems. invented three new methods applications based on assembly: "in data"...
Abstract Persian walnut ( Juglans regia ) and Manchurian mandshurica belong to Juglandaceae, which are vulnerable, temperate deciduous perennial trees with high economical, ecological, industrial values. 4-Coumarate: CoA ligase (4CL) plays an essential function in plant development, growth, stress. Walnut production is challenged by diverse stresses, such as salinity, drought, diseases. However, the characteristics expression levels of 4CL gene family species resistance under salt stress...
Drug resistance is a critical obstacle in cancer therapy. Discovering drug response important to improve anti-cancer treatment and guide design. Abundant genomic resources of cell lines provide unprecedented opportunities for such study. However, cannot fully reflect heterogeneous tumor microenvironments. Transferring knowledge studied from vitro single-cell clinical data will be promising direction better understand resistance. Most current studies include single nucleotide variants (SNV)...
Chlorella vulgaris is one of the most commonly used microalgae in aquaculture feeds. It contains high concentrations various kinds nutritional elements that are involved physiological regulation animals. However, few studies have been conducted to illustrate their influence on gut microbiota fish. In this work, Nile tilapia (Oreochromis niloticus) (average weight 6.64 g) was analyzed by high-throughput sequencing 16S rRNA gene after feeding with 0.5% and 2% C. additives diets for 15 30 days...
Abstract Bacterial infection poses a grave peril to human existence and well‐being, particularly with the emergence of drug‐resistant bacteria, leading an alarming rise in incidence severe illness mortality. Antimicrobial photodynamic therapy (aPDT) has emerged as one most promising strategies for combating bacterial infections due its potent bactericidal efficacy non‐inducement resistance. The development novel photosensitizers represents viable approach further enhance aPDT. Hence,...
The increase in the detection rate of synchronous multiple primary lung cancer (MPLC) has posed remarkable clinical challenges due to limited understanding its pathogenesis and molecular features. Here, comprehensive comparisons genomic immunologic features between MPLC solitary nodule (SN), as well different lesions same patient, were performed. Compared with SN, displayed a lower EGFR mutation but higher rates BRAF, MAP2K1, MTOR mutation, which function exactly upstream downstream...
Increasing evidence demonstrate that the gut microbiota is involved in pathogenesis of liver diseases, and faecal transplantation considered to be a promising new treatment option. However, there are no reports on intestinal flora asymptomatic HBV carriers using next-generation sequencing. This study intends investigate potential role microflora predicting progression Hepatitis B patients different non-cancerous stages. A total 266 with stages 31 healthy controls were included this study....
Lung cancer is a leading cause of mortality, highlighting the need for innovative non-invasive early detection methods. Although cell-free DNA (cfDNA) analysis shows promise, its sensitivity in early-stage lung patients remains challenge. This study aimed to integrate insights from epigenetic modifications and fragmentomic features cfDNA using machine learning develop more accurate model. To address this issue, multi-centre prospective cohort was conducted, with participants harbouring...
The inverse design of microstructures plays a pivotal role in optimizing metamaterials with specific, targeted physical properties. While traditional forward methods are constrained by their inability to explore the vast combinatorial space, offers compelling alternative directly generating structures that fulfill predefined performance criteria. However, achieving precise control over both geometry and material properties remains significant challenge due intricate interdependence. Existing...
Abstract Complicated molecular alterations in tumors generate various mutant peptides. Some of these peptides can be presented to the cell surface and then elicit immune responses, such are called neoantigens. Accurate detection neoantigens could help design personalized cancer vaccines. Although some computational frameworks for neoantigen have been proposed, most them only detect SNV‐ indel‐derived In addition, current adopt oversimplified prioritization strategies. These factors hinder...
Plant NAPDH oxidase (NOX) gene family members are critical producers of ROS and play crucial roles in plant signaling, development, stress responses. Opisthopappus taihangensis is a pivotal wild genetic resource Asteraceae. To date, little knowledge exists about the functions NOXs O. taihangensis. In this study, seven typical ferric reduction oxidases (FROs) were identified Dispersed duplication might role expansion NOX/FRO taihangensis, most homologous pairs have undergone purifying...
Deferred neural rendering (DNR) is an emerging computer graphics pipeline designed for high-fidelity and robotic perception. However, DNR heavily relies on datasets composed of numerous ray-traced images demands substantial computational resources. It remains under-explored how to reduce the reliance high-quality while maintaining fidelity. In this paper, we propose DNRSelect, which integrates a reinforcement learning-based view selector 3D texture aggregator deferred rendering. We first...
As a means of environmental enrichment, music environment has positive and beneficial effects on biological neural development. Kunming white mice (61 days old) were randomly divided into the control group (group C), D-tone D), A-tone A) G-tone G). They given different tonal stimulation for 14 consecutive (2 h/day) to study development hippocampus prefrontal cortex in early life its molecular mechanisms. The results showed that number neurons increased, with cell morphology relatively...
Disentangling how climate oscillations and geographical events significantly influence plants' genetic architecture demographic history is a central topic in phytogeography. The deciduous ancient tree species
The incidence of multiple primary lung cancer (MPLC) is increasing, with some our surgical patients exhibiting numerous lesions. We defined five or more lesions as super MPLCs. Elucidating the genomic characteristics this special MPLC subtype can help reduce disease burden and understand tumor evolution. In cohort synchronous early-stage MPLCs (PUMCH-ssesMPLC), whole-exome sequencing on 130 resected malignant specimens from 18 provided comprehensive super-MPLC landscapes. Mutations are...
Investigating mutations, including single nucleotide variations (SNVs), gene fusions, alternative splicing and copy number (CNVs), is fundamental to cancer study. Recent computational methods biological research have demonstrated the reliability significance of detecting mutations from single-cell transcriptomic data. However, there a lack single-cell-level database containing comprehensive mutation information in all types cancer. Establishing landscape huge emerging data can provide...
Triple-negative breast cancer (TNBC) is an aggressive subtype of BC characterized by extensive intratumoral heterogeneity. Compared with other types BC, TNBC more prone to invasion and metastasis. The aim the present study was determine whether adenovirus-mediated clustered regulatory interspaced short palindromic repeats (CRISPR)/Cas9 system capable effectively targeting enhancer zeste homolog 2 (EZH2) in cells lay experimental basis for investigation CRISPR/Cas9 as a gene therapy BC. In...